Domaine de Patsy

Os RAPDs e os ISSRs revelaram-se técnicas capazes de gerar marcadores que conseguem distinguir as cultivares e linhas de trigo em estudo. O dendrograma UPGMA de similaridade genética agrupou a maioria das cultivares de trigos moles por variedades botânicas, a maioria das cultivares de trigos rijos por espécie e as linhas de trigo ‘Barbela’ por regiões de colheita, quer para RAPDs quer para ISSRs.

No que concerne à caracterização morfológica e de produção foi observada elevada variabilidade genética entre cultivares e entre variedades botânicas. O dendrograma UPGMA de similaridade genética reflectiu essa variabilidade genética, uma vez que não se agruparam as cultivares de trigos moles por variedades botânicas, as cultivares de trigos rijos por espécies e as linhas de ‘Barbela’ por regiões de colheita.

Os marcadores morfológicos e de produção revelaram-se os menos eficazes, seguidos dos RAPDs e, finalmente, os ISSRs. Apesar dos marcadores morfológicos serem limitados em termos de caracteres e polimorfismo é de salientar a importância da sua utilização conjuntamente com diferentes tipos de marcadores moleculares para que a caracterização de germoplasma seja a mais completa possível.

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