Resonance energy transfer
SUBCELLULAR IMAGING OF DYNAMIC PROTEIN INTERACTIONS BY BIOLUMINESCENCE RESONANCE ENERGY TRANSFER.
... appropriate energy acceptor such as the yellow fluorescent protein (YFP) is present within resonance energy transfer permissive distances of Rluc, part of the energy can be transferred ...
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Frontiers Commentary onTallet et al. Investigation of prolactin receptor activation and blockade using time-resolved fluorescence resonance energy transfer A commentary on Investigation of prolactin receptor acti- vation and blockade using time-resolved fluorescence resonance energy transfer
... bioluminescence resonance energy transfer (BRET) and co-immunoprecipitation (co- IP) approaches, concluded that the PRLR constitutively homodimerize (or het- erodimerized when long and short iso- ...
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Thrombin detection in murine plasma using engineered fluorescence resonance energy transfer aptadimers
... Fluorescence Resonance Energy Transfer (FRET) aptadimer, is assembled with two aptamers, which target different epitopes of thrombin, interconnected with a DNA linker that contains a FRET couple and ...
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A Förster resonance energy transfer (FRET) approach for enhancing fluorescence contrast in phase-separated membranes
... Förster resonance energy transfer (FRET) between NBD-DHPE and TR-DHPE in the fluid ...acceptor. Energy transfer was characterized in detail using a combination of fluorescence ...
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Bioluminescence Resonance Energy Transfer as a Method to Study Protein-Protein Interactions: Application to G Protein Coupled Receptor Biology
... bioluminescence resonance energy transfer (BRET) approach involves resonance energy transfer between a light-emitting enzyme and fluorescent ...
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Comparing the quantification of Forster resonance energy transfer measurement accuracies based on intensity, spectral, and lifetime imaging
... 77 Massachusetts Avenue, NE47-279 Cambridge, Massachusetts 02139 Abstract. The measurement of Förster resonance energy transfer 共FRET兲 in microscopes can be realized by different imaging modali- ...
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A semiempirical approach to the intra-phycocyanin and inter-phycocyanin fluorescence resonance energy-transfer pathways in phycobilisomes.
... the energy to the photosynthetic reaction centers through a fluorescence resonance energy-transfer (FRET) ...the transfer of energy with an impressively high effi- ...
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Sensitive and mutiplexed microRNA quantification using amplified time-gated Förster resonance energy transfer
... Sensitive and Specific Nucleic Acid Detection Based on Rolling Circle Amplification and Förster Resonance Energy Transfer.. 15th Conference on Methods and Application[r] ...
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Time-resolved Multiplexed Förster Resonance Energy Transfer for Nucleic Acid Biosensing
... absorb energy from UV-Vis radiation and transfer it to the Ln 3+ ion, then result in the high luminescence of lanthanide complex which can reach to 10 4 -10 5 M −1 cm −1 , being essentially determined by ...
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Förster Resonance Energy Transfer Based Biosensor for Targeting the hNTH1–YB1 Interface as a Potential Anticancer Drug Target
... To further demonstrate that the FRET signals observed with Biosensor 1 constructs are the result of a specific interaction between hNTH1 and YB1DC, we performed FRET measurem[r] ...
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Development of a human breast-cancer derived cell line stably expressing a bioluminescence resonance energy transfer (BRET)-based phosphatidyl inositol-3 phosphate (PIP3) biosensor.
... of Luc-Akt-PH to the plasma membrane results in an energy transfer between the luciferase and the YFP (BRET) that can be monitored in living cells cultured in 96 well plates (Fig. 1A). This assay ...
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Real-time analysis of agonist-induced activation of protease-activated receptor 1/Galphai1 protein complex measured by bioluminescence resonance energy transfer in living cells.
... the energy transfer within the pre-assembled receptor-G protein complex after activation, rather than an increase in the number of G proteins interacting with the ...
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Förster Resonance Energy Transfer Immunoassays Using Engineered Proteins for Breast Cancer Biomarker Detection
... F IGURE 2.10: Representative QD core materials and their emission ranges for the most studied types in the biological application. (Reprinted from [Medintz et al., 2005]) and a 4 nm CdSe QD has approximately 33 % of its ...
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Quantification of Cellular Deoxyribonucleoside Triphosphates by Rolling Circle Amplification and Förster Resonance Energy Transfer
... Because quantification of endogenous intracellular dNTPs from cell extracts cannot be performed by directly using the assay calibration curves measured in reaction buffer [r] ...
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Analysis of RXR/THR and RXR/PPARG2 heterodimerization by bioluminescence resonance energy transfer (BRET)
... As a positive control, a biotin-labeled 60 bp duplex bearing the Epstein-Barr Nuclear antigen 1 (EBNA-1) binding sequence was mutants excited by energy transfer (Em535) and BRET signal generated by the two ...
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Photoluminescence and Förster resonance energy transfer in elemental bundles of single-walled carbon nanotubes
... of energy transfer and its effects are now clearly established in SWNT ...SWNTs. Energy transfer should also appear as a new parameter in engineering the optical properties of ...exciton ...
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Excited States and Energy Transfer in G-Quadruplexes
... including resonance energy transfer, have been widely used to study structural and functional aspects of G-quadruplexes, 3,11,12 as well as their interactions with various ...
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Determination of Energy-Transfer Distributions in Ionizing Ion-Molecule Collisions
... The experiments have been performed at ARIBE, the low-energy ions facility of GANIL (Caen, France) and at the GASPHASE beamline of the synchrotron radiation facility ELETTRA (Trieste, Italy). Both experiments are ...
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Humic Substances Enhance Chlorothalonil Phototransformation via Photoreduction and Energy Transfer.
... electron transfer occurs between humic constituents them- selves, from the short-lived excited states of electron donors to the ground-state electron acceptors, leading to hydrogen peroxide ...can transfer ...
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Membranes: A Variety of Energy Landscapes for Many Transfer Opportunities
... The energy to overcome the barrier comes from the drag force (the permeate flux) that is transmitted on all the accumulated particles (the drag force must be multiplied by the number of accumulated layers or by ...
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