High-throughput DNA sequencing
Propidium monoazide (PMA) and ethidium bromide monoazide(EMA) improve DNA array and high-throughput sequencing ofporcine reproductive and respiratory syndrome virus identification
... with the same superscript number must be compared only together. DNA array probes relative intensity from A) lung tissue homogenates spiked 707. with PRRSV (5,000 TCID50/mL), B) lung ti[r] ...
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Isolation of Plasmodium falciparum by flow-cytometry: implications for single-trophozoite genotyping and parasite DNA purification for whole-genome high-throughput sequencing of archival samples.
... free DNA is fixed. Thus the presence of DNA causes their fluoresce and can be misdiagnosed as trophozoite nuclei ...of DNA within trophozoite ...
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Non-invasive monitoring of diffuse large B-cell lymphoma by cell-free DNA high-throughput targeted sequencing: analysis of a prospective cohort
... Patients’ examples As an example, two emblematic patients were more extensively described (Fig. 6 ). Patient #5 had one of the highest MTVs in its baseline PET scan (2441 cm 3 ), with an important dissemination of the ...
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BIC-seq: a fast algorithm for detection of copy number alterations based on high-throughput sequencing data
... 2010 DNA copy number alterations (CNA), which are amplifications and deletions of certain regions in the genome, play an important role in the pathogenesis of cancer and have been shown to be associated with other ...
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Gene Capture Coupled to High-Throughput Sequencing as a Strategy for Targeted Metagenome Exploration
... next-generation sequencing (NGS) technologies recover genetic materials from en- vironmental samples without the preparation of meta- genomic clone ...higher throughput and lower costs than other ...coding ...
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High-throughput detection of clinically targetable alterations using next-generation sequencing
... Next-generation sequencing (NGS) has transformed the cancer genomics landscape by enabling comprehensive cancer genome characterization of unprecedented scope [7, ...and high- throughput sample ...
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A tunable filter for high molecular weight DNA selection and linked-read sequencing
... for DNA size selection 20 ...of DNA for ...the throughput of the filter is scalable by parallelizing the technology in a multicapillary system of 61 ...of high MW genomic DNA in two ...
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High-throughput microsatellite isolation through 454 GS-FLX Titanium pyrosequencing of enriched DNA libraries
... a DNA 7500 chip (Agilent ...single-stranded DNA library ...single-strand DNA profile and quantification were determined by running 1 lL of each sample on an Agilent Bioanalyzer 2100 with a RNA Pico ...
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Analysis of High-Throughput Sequencing and Annotation Strategies for Phage Genomes
... sheared DNA fragments of low molecular weight (#200–250 bp) was achieved using the AMPure Kit (Agencourt Bioscience Corporation, Beverly MA), following exactly the manufacturer’s recommenda- tions, with a sample ...
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Vidjil: A Web Platform for Analysis of High-Throughput Repertoire Sequencing
... deep sequencing strategy. While DNA-Seq sequencing with specific V(D)J primers usually lead to more than 95% analyzed sequences, capture with many probes or RNA-Seq strategies usually lead to ...
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SESAME (SEquence Sorter & AMplicon Explorer): genotyping based on high-throughput multiplex amplicon sequencing
... 1). DNA sequences are read from one single FASTA file (step ...of DNA markers with corresponding reference sequences that will be used for assignation of the reads to loci and for trimming them from primers ...
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Virus Detection by High-Throughput Sequencing of Small RNAs: Large-Scale Performance Testing of Sequencing Analysis Strategies
... various DNA pararetroviruses and corresponded to BLAST annotations of contigs that likely represent endogenous viral elements (EVEs) known to be integrated in plant genomes and represented in the population of ...
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Study of bacterial populations from oligotrophic soil ecosystems using high throughput sequencing technologies
... Generation DNA Sequencing (NGS) and bioinformatic tools Next generation sequencing platforms allow analysis of hundreds of environmental samples in a single sequencing run ...Various ...
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Mapping of quantitative trait Loci underlying cold tolerance in rice seedlings via high-throughput sequencing of pooled extremes
... and sequencing A segment of fresh leaf ...extract DNA using the CTAB method [31]. The two DNA pools were purified with the GenElute Plant Genomic DNA Miniprep Kit (Sigma-Aldrich, ...genomic ...
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Compression of Structured High-Throughput Sequencing Data
... of high-throughput sequencing ...new sequencing or analysis methods (because they do not support schema evolution), or fail to provide state of the art compression of the ...or DNA ...
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A Primer on the Analysis of High-Throughput Sequencing Data for Detection of Plant Viruses
... in high-quality viral RNA and DNA and allows the use of up to several hundred grams of starting ...for high throughput, and relying on numerous laboratory operations, the approach only ...
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Marine protist diversity in European coastal waters and sediments as revealed by high-throughput sequencing
... our DNA/RNA ratios could reflect different genomic architectures among taxonomic groups, and not necessarily different relative ...with DNA that can be preserved in dead cells or in the dissolved 320 ...
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High-Throughput Sequencing of Transposable Element Insertions Suggests Adaptive Evolution of the Invasive Asian Tiger Mosquito Towards Temperate Environments
... candidate loci that could be directly used to answer this question in Ae. albopictus DNA samples. As with every novel method, our study may be susceptible to unforeseen or underrated bias; although we attempted to ...
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Cost-effective high-throughput single-haplotype iterative mapping and sequencing for complex genomic structures
... single-‐haplotype DNA are ...using DNA from a hydatidiform mole, an abnormal conceptus that arises when an enucleated egg is fertilized by a single X-‐bearing sperm 17-‐19 ...of DNA for library ...
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Reference-free compression of high throughput sequencing data with a probabilistic de Bruijn graph
... compression of FASTA and FASTQ files, including read IDs, DNA sequences and quality scores. Sequence compression techniques fall into two cate- gories: reference-based methods, such as Q UIP , CRAM, P ATHENC and ...
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