[PDF] Top 20 The hCMEC/D3 cell line as a model of the human blood brain barrier.

... date, the main advantage of the hCMEC/D3 cell line is that it represents a stable, easily grown and transfer- able population of human microvascular ... Voir le document complet

... immortalized brain capillary endothelial cells (hCMEC/D3) have recently been developed as an in vitro human BBB model ...This cell line has been now exten- sively ... Voir le document complet

... levels of AhR transcripts have previously been reported in both isolated human brain cortex microvessels and in the hCMEC/D3 human cerebral microvascular endothelial ... Voir le document complet

... analysis of HIV-1 intra-endothelial cell migration. hCMEC/D3 cells ...to the cell monolayers for 4 h at ...4°C. The intracellular immunostaining was performed after ... Voir le document complet

... organization of BBB spheroids. To investigate the spontaneous assembly and formation of the BBB spheroids, we co- cultured primary human astrocytes and human brain ... Voir le document complet

... impact of a substance on BBB structure and function can be induction of widespread leakiness and enhanced permeability or activation of the endothelium leading to disrupted regulation ... Voir le document complet

... characterization of i-BECs. he i-BECs were evaluated in a two-compartment in vitro Transwell BBB transport ...culture, the i-BECs were dissociated and seeded at a density of ... Voir le document complet

... infection of the i-BECs was identi- ied 24 h post-exposure to the virus by immunoluo- rescence ...2 a, b) and was detected in cell lysates by Western blotting after only a 2-h ... Voir le document complet

... importance of TWEAK in brain pathology is fur- ther evidenced by data proving that TWEAK blocking antibodies or Fn14 decoy receptors are efficient in ani- mal models of ischemic stroke and ... Voir le document complet

... studies of brain endothelial cells in vivo and in vitro demonstrate the coexistence of an additive carrier-mediated process along with the passive diffusion flux of cocaine at ... Voir le document complet

... Within the complex interplay between the gut microbiome and the CNS, a role for brain barriers and neuroinflammation is becoming important ( Braniste et ...). The impact ... Voir le document complet

... tion of antibody therapeutics and targeted gene therapies necessitates humanized preclinical models to circumvent species-related difer- ences in immune systems, receptor expression and antibody cross-reac- ... Voir le document complet

... in the focal ischemic brain in an animal model of stroke (Sims et ...inhibition of Shh signaling leads to aggravated brain edema in an animal model for acute ischemic ... Voir le document complet

... upon the DM is consistent but becomes more important upon US treatment suggesting that increased electrical conductivity of the US treated membrane has a damping effect on permeability ... Voir le document complet

... Modelling the BBB In vitro models that can accurately mimic the BBB in culture are crucial tools for the development of brain-permeable drugs at the pre-clinical ...facilitate ... Voir le document complet

... steps of vasculogenesis, angiogenesis, and in some organs, ...In the CNS, the blood- brain barrier (BBB) is composed of tightly bound endothelial cells (ECs) which ... Voir le document complet

... detect the presence of cells and biomolecules. Specifically, when a cell is in the proximity of an OECT channel, its membrane is polarized, resulting in an additional ... Voir le document complet

... 2003). The expression of the outer membrane protein A (OmpA) is critical for entry and survival inside both PMNs and ...paralog of heat shock pro- tein 90, which may gain extracellular ... Voir le document complet

... Alterations of expression and localization of TJ proteins Caveolae are specialized plasma membrane microdo- mains, abundantly found in endothelial cells where they mediate various biological events such as ... Voir le document complet

... 4 Cell‑Produced Delivery Vehicles: Promise of Extracellular Vesicles Exosomes have recently emerged as novel lipid nanocarriers for the delivery of small molecule drugs, protein- [ 81 , 82 ], ... Voir le document complet