Post-translational protein modification
An extended dsRBD is required for post-transcriptional modification in human tRNAs
... CONCLUSION Using biochemical analysis and structural characterization, we here show that HsDus2 uses a unique strategy for bind- ing and modifying its tRNA substrate. In contrast to other Dus proteins, HsDus2 has a ...
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Quantitative proteomics methods for the analysis of histone post-translational modifications
... 1.3.1. Basic steps in histone PTM analysis by mass spectrometry 1.3.1.1. Proteolytic digestion Enzymes that cleave peptide bonds in proteins are called proteases [173]. Trypsin is the most commonly used enzyme for ...
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Conformational flexibility of coenzyme A and its impact on the post‐translational modification of acyl carrier proteins by 4′‐phosphopantetheinyl transferases
... As mentioned above, only limited structural informa- tion on family II PPTases at neutral pH is available. We were then eager to see how a physiologically rele- vant pH could influence the binding of CoA and diva- lent ...
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Post-transcriptional regulation during spermatogenesis : Role of the RNA-Binding Protein HuR
... 9: Post-transcriptional regulation by microRNAs and small interfering RNAs RNA interference (RNAi) and microRNA (miRNA) pathways are evolutionarily conserved control mechanisms that use RNA molecules to inhibit ...
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Control of nuclear receptor activities in metabolism by post-translational modifications.
... Ubiquitin-like Modification (SUMOylation) is an important PTM which modulates positively or negatively the activity, the stability and the localization of intracellular ...covalent modification by SUMO ...
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Post-protein binding metal-mediated coupling of an acridine orange-based fluorophore
... the x-ray structure of the metalloprotein. 35,36 We note that coordination of AO to Lys1 did not significantly affect the nCO frequencies of the fac-[Re(CO)3] + core in Lys2. The UV-visible and the emission spectra of ...
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The translational landscape of Arabidopsis mitochondria
... of post-transcriptional modifications ( 3 , 4 ...and protein composition of mitoribosomes differ ex- tensively from those of Escherichia coli ribosomes ( 7 , 8 ...
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Translational machinery of the chaetognath Spadella cephaloptera: a transcriptomic approach to the analysis of cytosolic ribosomal protein genes and their expression
... Eukaryotic RP genes appear to belong to multigene fami- lies [27,28]; however, great differences have been found between the different Kingdoms. In the yeast cell, where approximately half of the RP genes are duplicated ...
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Analytical strategies for the comprehensive profiling of histone post translational modifications by mass spectrometry and implications for functional analyses
... 5.2 Introduction Eukaryotic DNA is packaged into a nucleoprotein structure known as chromatin. The primary function of this structure is to compact DNA within the cell nucleus. The fundamental repeating unit of ...
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O-mycoloylation : caractérisation d'une nouvelle modification post-traductionnelle de petites protéines d'enveloppe chez les Corynebacterineae
... either. We thus concluded that NCgl0187 is likely an essential gene. Glycosyl compositional analysis of the cell wall and AG from Cg- D 186 and ATCC 13032 strains Purified cell walls from Cg-D186 and its parental ATCC ...
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Post-transcriptional regulatory patterns revealed by protein-RNA interactions
... www.nature.com/scientificreports www.nature.com/scientificreports/ targets, indicating that they may be regulated by the binding of certain RBPs and the avoidance of others. Finally, 50 functional units (~17%, D-FU set) ...
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Toolbox of post-synthetic mordenite modification strategies : Impact on textural, acidic, and catalytic properties
... Figure IV.13. Molar yields as a function of the initial conversion for toluene disproportionation of catalyst series: M10 (red symbol), Na x (violet), F x (brown), and PyF x (yellow). The deactivation is much more ...
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Total Roto-Translational Variation
... In computer science, similar ideas were successfully implemented in discrete graphs (with nodes representing a spatial point and orientation) in order to minimize curvature-dependent contour energies [60, 33, 32, 62, 44, ...
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Total roto-translational variation
... Keywords: Image processing, shape processing, image inpainting, curvature, Elastica, roto- translations, convex relaxation, total variation.. AMS MSC (2010): 53A04 49Q20 26A45 35J35 53A4[r] ...
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Anomalous Behaviour of a Protein During Sds/Page Corrected by Chemical Modification of Carboxylic Groups
... According to its amino acid sequence (Houba et al., 1989), the protein contains 41 glutamic acid and 24 aspartic acid residues compared with two lysine, 14 arginine and five histidine re[r] ...
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Proteomic approaches for the detection of unusual post-translational modifications in simple and complex bacterial protein mixtures
... However, only the MS/MS spectrum offlageliin (m/z H54. charge state +48) showed characteristic carbohvdrate oxonium ions in the low mass region (Fig. 4.29a) whereas these characteristic [r] ...
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The archaeal origins of the eukaryotic translational system
... Using the alignable regions of all 67 ribosomal proteins common to Eukarya and Archaea, as well as various subsets, probable phylogenetic relationships were constructed, as de- scribed in Materials and methods. First, as ...
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Translational Research and Therapeutic Perspectives in Dysferlinopathies
... transmembrane protein, named dysferlin. This protein belongs to the fer- lin family, which includes otoferlin, myo - ferlin, fer-1-like 5 (FER1L5) and the origi- nally identified Caenorhabditis elegans ...
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Determinants of translational efficiency in Saccharomyces cerevisiae
... or modification of the s 2 moiety leads to a reduction in the rate of tRNA charging in vitro (Sen and Ghosh, 1976; Seno et ...tRNA modification was discovered (Huang et ...insufficient protein ...
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Construction a RBS library with different translational activity
... fluorescence protein on a low copy number ...reporter protein activity has a positive correlation to RBS translational activity, we can design customizable RBS translational strengths for ...
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