High-Throughput Nucleotide Sequencing
UMD-Predictor: A High-Throughput Sequencing Compliant System for Pathogenicity Prediction of any Human cDNA Substitution
... genome sequencing (WGS) experiments have revealed that every human carries about 3 million single-nucleotide polymorphisms (SNP) [Levy et ...next-generation sequencing (NGS) technologies are widely ...
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Mapping of quantitative trait Loci underlying cold tolerance in rice seedlings via high-throughput sequencing of pooled extremes
... Data processing and SNP identification The raw DNA-seq reads were trimmed and filtered to remove low-quality sequences via a modified Mott trimming algorithm implemented by a custom Perl script that we wrote by referring ...
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Compression of Structured High-Throughput Sequencing Data
... European Nucleotide Archive, was developed to try and compress HTS alignments better than can be achieved with ...rapidly, sequencing throughput increases exponentially and new experi- mental ...
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High-throughput detection of clinically targetable alterations using next-generation sequencing
... DSTP validation in a clinical molecular diagnosis cohort Next, 140 samples from three different tumor locations (lung, colorectal, and skin; Table 3) that were previously analyzed by conventional methods for the ...
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Vidjil: A Web Platform for Analysis of High-Throughput Repertoire Sequencing
... Fig 3. Interactive analysis of the CDR3 length distribution for the TR γ locus with the Vidjil web application. The publicly available L4 dataset is a diagnosis sample of an acute lymphoblastic leukemia patient (dataset ...
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High throughput error corrected Nanopore single cell transcriptome sequencing
... PromethION sequencing run would require the generation and analysis of about 10 14 barcode sequences for ED = ...16 nucleotide cellBC into just one ...two sequencing errors in the cellBC, can differ ...
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Cost-effective high-throughput single-haplotype iterative mapping and sequencing for complex genomic structures
... screening high-‐dimensional pools of BACs for STS content by ...end-‐ sequencing or PCR will exceed the cost to obtain draft sequence using the SHIMS ...making sequencing the most efficient way to ...
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High-throughput transcriptome sequencing and preliminary functional analysis in four Neotropical tree species
... Gene Ontology analysis provided the annotation of all contigs with significant Blast hits. Additional file 9: Tables S3 and Additional file 10: Table S4 report respectively contig se- quences and grouping of contigs by ...
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Virus Detection by High-Throughput Sequencing of Small RNAs: Large-Scale Performance Testing of Sequencing Analysis Strategies
... Analyses of viral contigs. These analyses were carried out using the contigs reported as viral by each participant and their characteristics (number of reads and sequence). To evaluate the ability of each pipeline to ...
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GECKO is a genetic algorithm to classify and explore high throughput sequencing data
... recently high throughput sequencing (HTS), have considerably advanced knowledge of disease etiology and classification 1 – 3 ...of nucleotide sequences of length k between samples 5 ...RNA ...
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Next Generation High Throughput Satellite System
... Wideband carrier operation DVB-TM-S2 group has been working lately on wideband satellite transponders aiming to increase baud rates up to 200/500 Mbaud. This proposal aims to achieve more efficient payload operations, ...
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Current impact and future direction of High Throughput Sequencing in plant virus diagnostics: the drivers of COST Action FA1407
... 2 NGS & Viruses Etiology Viral ecology Plant-virus interaction Plant resistance Viral population genetics Taxonomy Current impact on R&D?. Expected impact on diagnostics?[r] ...
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High-Throughput Sequencing of Transposable Element Insertions Suggests Adaptive Evolution of the Invasive Asian Tiger Mosquito Towards Temperate Environments
... that invasive temperate populations came from the northernmost territories of the native area such as northern China or Japan where Ae. albopictus populations are already cold-adapted. It would be thus interesting to ...
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A Primer on the Analysis of High-Throughput Sequencing Data for Detection of Plant Viruses
... obtain high-quality nucleic acids intended for HTS [ 10 – 13 ...RNA sequencing might be most straight- forward and universal to use for single ...for sequencing of pools of many samples, or to ...
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Marine protist diversity in European coastal waters and sediments as revealed by high-throughput sequencing
... from the same species (Pizay et al., 2009). Accurate identification may thus not always be discerned from cell morphology alone, and this is more critical for protists below 20 µm in size that often lack conspicuous ...
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BIC-seq: a fast algorithm for detection of copy number alterations based on high-throughput sequencing data
... Next-generation sequencing technologies provide an opportunity to identify CNA regions with unprece- dented ...whole-genome sequencing data for samples with matched ...
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Illuminating an Ecological Blackbox: Using High Throughput Sequencing to Characterize the Plant Virome Across Scales
... Montpellier University, Montpellier, France, 5 Department of Plant Biology and Graduate Program in Ecology, Evolution and Behavior, Michigan State University, East Lansing, MI, United States, 6 Laboratory of Virology, ...
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High-Resolution Chromatin Immunoprecipitation: ChIP-Sequencing.
... Fragmented protein-DNA complexes are then selectively immunoprecipitated using protein specific antibodies. The quality and specificity of antibodies used for this assay is of utmost importance to prevent non-specific ...
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Computational methods for high-throughput pooled genetic experiments
... 1.2 Previous work Pooled analysis of separate populations has been proposed and implemented for multiple traits and organisms. The primary motivation for pooled analysis is the cost savings of per- forming fewer ...
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Propidium monoazide (PMA) and ethidium bromide monoazide(EMA) improve DNA array and high-throughput sequencing ofporcine reproductive and respiratory syndrome virus identification
... with the same superscript number must be compared only together. DNA array probes relative intensity from A) lung tissue homogenates spiked 707. with PRRSV (5,000 TCID50/mL), B) lung ti[r] ...
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