Souris :
>es souris MMds-Eeu de fond généƟƋue >ͬc obtenues du laboratoire du Wrof͘ &ede-rica avallo et les souris caspase-1 obtenues du laboratoire du Wr͘ Zichard ͘ &lavell ont été croi-sées aĮn d͛obtenir des souris caspase-1/caspase-11-ͬ- MMTV-Neuн et caspase-1/caspase-11нͬн
MMTV-Neuн͘ >͛appariƟon de tumeurs est surveillée dans les glandes mammaires trois fois par
semaine dés le sevrage des souris femelles͘ Une masse dont le plus grand des diamètres edžcède 3mm est considérée comme une tumeur͘
InũecƟon d͛AnaŬinra :
Une soluƟon d͛anaŬinra diluée dans du WS est réalisée à 4mgͬm>͘ Un ũour avant l͛inũec-Ɵon des cellules 4d1͕ la souris sont traitées en /W avec 2Ϭmgͬg d͛naŬinra (volume с 5 Ύ poids de la souris en mgͿ͘ >es traitements sont eīectués ensuite tous les 2 ũours en /W͘ omme pour les autres edžpériences de croissance tumorale͕ 1Ϭ ϬϬϬ cellules 4d1 (1ϬϬ ʅ>Ϳ sont inũectées dans la glande mammaire et le suivi des tumeurs est eīectué par mesure au pied à coulisse͘
Culture cellulaire :
>es cellules K771 sont culƟvées dans du milieu M M supplémenté avec 1Ϭй (vͬvͿ de sérum de veau fƈtal inacƟvé͕ 1й (vͬvͿ glutamadž͕ 1й (vͬvͿ pénicillineͬstreptomLJcine dans un incubateur à 5й K2 et 37Σ͘ vant inũecƟon͕ les cellules sont sLJstémaƟƋuement testées pour la présence de mLJcoplasmes (MLJcolert MLJcoplasma detecƟon Ŭit͕ >onnjaͿ͘ >es lignées sont aussi saines de tout agents infecƟeudž murins (daƋman WZ tesƟng of mouse essenƟal panel͕ harles ZiverͿ͘
InũecƟons des cellules EKϳϳ1 :
>es cellules K771 sont récoltées et reprises dans du WS à 2͘1Ϭϲ cellulesͬm>͘ 1ϬϬ ʅ> de ceƩe soluƟon sont inũectées directement dans la glande mammaire de souris 57lͬϲ td ou
caspase-1 <K͘ >a croissance des cellules est suivie par mesure au pied à coulisse͘ >es souris sont
14Ϭ
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