Yeast two-hybrid
Yeast two-hybrid, a powerful tool for systems biology.
... The Yeast Two-Hybrid Approach ...the yeast Saccharomyces cerevisiae [17]. Until then, interactions between two proteins had mostly been studied using biochemical ...into two ...
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2HybridTools, a handy software to facilitate clone identification and mutation mapping from yeast two-hybrid screening
... ABSTRACT Yeast Two-Hybrid (Y2H) and reverse Two-Hybrid (RY2H) are powerful protein– protein interaction screening methods that rely on the interaction of bait and prey proteins fused to ...
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Flavivirus NS3 and NS5 proteins interaction network: a high-throughput yeast two-hybrid screen.
... high-throughput yeast two-hybrid (Y2H) ...high-throughput yeast two-hybrid (Y2H) technique to conduct genome-scale screens of virus-host protein interactions has led to major ...
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A Quantitative Tri-fluorescent Yeast Two-hybrid System: From Flow Cytometry to In cellula Affinities
... performed in NuPAGE™ MOPS SDS Running Buffer (Thermo Scientific). Proteins were then transferred on a Nitrocellulose Membrane 0.45 µm (Biorad), using a Trans-Blot® Turbo™ Transfer System (Biorad) for 14 minutes, at 1 A ...
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Uncovering novel protein partners of nucleolar protein 6 (Nop6) by yeast two-hybrid analysis and their role in ribosome biogenesis
... A network based algorithm of large scale data to assign gene function predicted N op6 to be a fungal-specific nucleolar prote in involved in rRNA processing (Samanta &[r] ...
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A yeast two-hybrid knockout strain to explore thioredoxin-interacting proteins in vivo
... trx2⌬ Yeast Strain as a Tool to Reveal Essential Cysteines in TRX ...contains two cysteine residues: C112, which does not possess any catalytic activity (46), and C245 in the consensus sequence KxECG(L ...
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Two-hybrid screening of FAM13A protein partners in lung epithelial cells
... Main text Methods Yeast two‑hybrid analysis Yeast two-hybrid screening was performed by Hybri- genics Services, S.A.S., Paris, France ( http://www.hybri genic s-servi ces.com ). ...
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Analysis of yeast metabolomics by LC-MS
... However, so far the who le metabolome coverage can ' t be achieved by any of the analytical platforms or methods due to the large variety of chemical and physi[r] ...
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Hybrid Dimensional Modelling and Discretization of Two Phase Darcy Flow through DFN in Porous Media
... Table 3: N ∆t is the number of successful time steps; N N ewton is the total number of Newton iterations (for successful time steps); N GM Res is the total number of GMRes iterations (for successful time steps); N Chop ...
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Natural single-nucleosome epi-polymorphisms in yeast.
... the two assembled genome sequences and used probe positions to fit a Hidden Markov Model (HMM) for inference of nucleosome positioning in each strain, as previously described [25] (Figure ...the two strains ...
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Phosphoproteomic Analysis of Isolated Mitochondria in Yeast
... phosphatases in last years suggests that reversible protein phosphorylation plays an important part in the control of mitochondrial processes. In addition, many mitochondrial phosphoproteins probably still remain to be ...
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Vertex Approximate Gradient Scheme for Hybrid Dimensional Two-Phase Darcy Flows in Fractured Porous Media
... Figure 13: Discrete solution obtained by the VAG-1 scheme with the first mesh i mesh = 1 at final simulation time: oil saturation in the fracture network and in the matrix using the lower threshold in the matrix equal ...
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Vertex Approximate Gradient Scheme for Hybrid Dimensional Two-Phase Darcy Flows in Fractured Porous Media
... such that Γ i = S σ ∈F Γi σ . We will denote by F Γ the set of fracture faces S i∈I F Γ i . This geometrical discretization of Ω and Γ is denoted in the following by D. The VAG discretization has been introduced in [3] ...
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Reshuffling yeast chromosomes with CRISPR/ Cas9
... We also showed that genome reshuffling generates a great phenotypic diversity under spe- cific environmental conditions including many cases of fitness advantage ( Fig 5E ). Similar findings were previously described ...
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Aneuploidy Drives Genomic Instability in Yeast
... wild-type yeast, loss of REV3 confers only a slight increase in genotoxin sensitivity, as recombinational repair is sufficient to replicate past most lesions ...bleomycin, two double-strand break ...
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10th Francophone Yeast Meeting 'Levures, Modèles & Outils'
... websites. Two examples of genome sequencing and analysis readily meeting these criteria were presented, both from biotechnologically relevant ...first two genes of the biotin biogenesis pathway, enabling ...
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A Universal Approach toward Light-Responsive Two-Dimensional Electronics: Chemically Tailored Hybrid van der Waals Heterostructures
... We first demonstrate the light-controllable doping of BP FET by AZO molecules (device structure shown in Figure 5a, channel length/width is 2.2 μm/3.3 μm). Compared with BP FET without molecules, the adsorption of ...
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Hybrid (Bolted/Bonded) Joints Applied to Aeronautic Parts: Analytical Two-Dimensional Model of a Single-Lap Joint
... The load transfer is rather confined to the extremities of the joint. Hence the edge distance is highly influent relatively to the influence of the longitudinal pitch. Thanks to the presented model, the influence study ...
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Hybrid (bolted/bonded)joints applied to aeronautic parts: analytical two-dimensional model of a single-lap joint
... analytical two-dimensional model of hybrid (bolted/bonded) joint in the balanced single-lap configuration is developed and presented in this ...stiffnesses. Two approaches are suggested in order to ...
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Hybrid Organizations
... Governance mechanisms What is clear, though, is that hybrid arrangements need monitoring. This is so because of high risks of opportunistic behaviour and free riding, which is the dark side of the forces pushing ...
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