BIOFUTUR 289 • JUIN 2008 62
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White wells can improve qPCR results
Every researcher doing real-time PCR always wonders about the best instrument, the best mastermix, the best polymerase etc. However, when it comes to the plastic consumables that build the connection between ins- truments and reagents, one does not expect any influence on the final results. Eppendorf can demons- trate significant improvement of real-time PCR data using a new PCR plate. With this new twin.tec real- time PCR plate, Titanium dioxide gives the reaction chambers a bright white, opaque colour. This leads up to a 10-fold increase in reflection of fluorescence, which provides better data for cutting-edge applications such as low volume qPCR. Due to the more homogenous background of the white colour compared to clear wells, data from replicates becomes more homogenous. This results in lower standard deviation over a plate, which can be crucial to further process the data in multiva- riate analyses.
For further information:
www.eppendorf.com/realtime
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Eppendorf concentrates on versatility plus ease of use
Pre-selection of one of four different heating levels allows optimised drying times for diffe- rent samples, while three application modes – for aqueous, alcohol or high vapour pressure solvents – provide faster evaporation, reducing processing time by up to 20 per cent.
The dessicator function can be used for fast evapo- ration of filters and the 1,400 rpm centrifugation func- tion is ideal for spinning down droplets after re-suspension. Eppendorf supplies 14 different com- patible rotors, ranging from 0.2 ml PCR tubes to 25 ml tubes, and increased throughput is possible using two stacked rotors.
According to Peter Schreiner, Product Manager Multi- purpose Centrifuges, Eppendorf AG, Hamburg, “Hand- ling samples up to 240 ml, the new Concentrator plus offers a compelling alternative to concentration by freeze drying, rotary evaporation, precipitation or evapora- tion by nitrogene stream. Target applications are those requiring fast, gentle concentration of DNA, RNA, oli- gonucleotides and proteins, including small volume and temperature-sensitive samples.”
The Eppendorf Concentrator plus is available in a choice of two configurations: the basic device or a complete system with integrated service-free dia- phragm pump, vapour condenser and optional junc- tion to connect an external device such as a gel dryer.
The compact design saves on bench space and clear allocation between soft keys and the digital display ensures easy, intuitive use.
For further information:
www.eppendorf.com/concentrator
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