[PDF] Top 20 Targeted mutagenesis in wheat microspores using CRISPR/Cas9
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Targeted mutagenesis in wheat microspores using CRISPR/Cas9
... www.nature.com/scientificreports Targeted mutagenesis in wheat microspores using CRISPR/Cas9 Pankaj Bhowmik 1 , Evan Ellison 2 , Brittany Polley 1 , Venkatesh ... Voir le document complet
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Knockdown of human AMPK using the CRISPR-Cas9 genome-editing system
... of Cas9 to the targeted genomic DNA sequence leading to DNA double strand breaks ...(DSB). In case of DSB repair by non homologous end joining (NHEJ), small insertions/deletions within coding ... Voir le document complet
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CRISPR-Cas9-mediated efficient directed mutagenesis and RAD51-dependent and RAD51-independent gene targeting in the moss Physcomitrella patens
... at targeted genomic locations, using homing endonucleases such as meganucleases, zinc finger nucleases (ZFNs) and TAL effector nucleases ...II CRISPR-Cas9 (clustered regularly interspaced ... Voir le document complet
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CRISPR/Cas9 cleavage of viral DNA efficiently suppresses hepatitis B virus
... while Cas9/sg17 was efficient in suppressing infection and in directly cleaving nuclear cccDNA, Cas9/sg21 efficiently cleaved only integrated but not episomal DNA, which resulted in a ... Voir le document complet
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An optimised CRISPR/Cas9 protocol to create targeted mutations in homoeologous genes and an efficient genotyping protocol to identify edited events in wheat
... a Cas9 nuclease, which originated from ...pFGC-pco- Cas9, was a gift from Jen Sheen (Addgene plasmid # 52256 [ 19 , 35 ]); it was used in combination with the pair of gBlocks for editing of ... Voir le document complet
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Correction de mutations causant l’épidermolyse bulleuse simplex par recombinaison homologue avec la technologie CRISPR/Cas9
... de CRISPR/Cas9 dans l’épidermolyse bulleuse dystrophique ...de CRISPR/Cas9 est variable, tel qu’observé dans le présent projet de recherche, lors de la vérification de la RNH dans les cellules ... Voir le document complet
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Utilisation des technologies CRISPR/Cas9 pour le développement d'approches thérapeutiques pour le traitement de la dystrophie musculaire de Duchenne
... exons in the DMD gene coding for the dystrophin protein thus abrogating the synthesis of this protein in muscle cells [3, ...death in the early twenties ...the CRISPR-Cas9 technology ... Voir le document complet
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Correction de mutations causant l'épidermolyse bulleuse simplex par recombinaison homologue avec la technologie CRISPR/Cas9
... avec CRISPR/Cas9 Depuis plusieurs années maintenant, la professeure-chercheure Catherine Laprise de l’Université du Québec à Chicoutimi réalise des projets de recherche portant sur ... Voir le document complet
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Inativação do gene da fitoeno desaturase do mamoeiro por CRISPR/Cas9
... por CRISPR/Cas9 (XING, ...enzima Cas9 e uma sequência referente a estrutura do gRNA, onde 19 nucleotídeos desta sequência é customizado de acordo com a sequência alvo do genoma da planta que se ... Voir le document complet
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Adenovirus-Mediated Somatic Genome Editing of Pten by CRISPR/Cas9 in Mouse Liver in Spite of Cas9-Specific Immune Responses
... characterizing Cas9-specific host immune re- sponses following gene delivery, and the develop- ment of needed precautions or workarounds will be necessary features in future translational ...delivery ... Voir le document complet
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CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling
... packaged using AAV9 (AAV9-KPL) and delivered i.t. into Cre-dependent Cas9 mice, with a relatively low dose of virus for sparse ...indels in p53 and LKB1 at the predicted cutting site, with ...indels ... Voir le document complet
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CRISPR-Cas9 pour l'édition de génomes viraux et l'étude des gènes du phage virulent p2
... ADN in vivo doit se faire au cours d’un court cycle d’infection qui parfois ne dure que quelques ...systèmes CRISPR-Cas pour l’édition de génome de phages (Box et ...l’outil CRISPR-Cas9 ... Voir le document complet
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Pépite | Essais cliniques « first in man », application clinique de la méthode d’édition du génome crispr/cas9
... par CRISPR/Cas 9, sera de revenir en profondeur avec l’équipe investigatrice sur le circuit du médicament et sur les actes de préparation ou d’administration ...first in man chez des ... Voir le document complet
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A CRISPR/Cas9 platform for MS2-labelling of single mRNA in live stem cells
... site in 67 +- 4% of the cells (average +- range of three biological replicates with n > 100 cells each) ...expressed in mouse embryonic stem cells, but down-regulated during differentiation towards ... Voir le document complet
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L'utilisation du système CRISPR-Cas9 pour l'étude des protéines non structurales du bactériophage 2972 infectant Streptococcus thermophilus
... Les systèmes sont composés de loci d’un nombre variable de courtes séquences nucléotidiques identiques, nommées répétitions, entrecoupées par des espaceurs uniques de longueurs variables selon l’espèce (figure 1.9). Les ... Voir le document complet
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en fr Axonal target specificity in the CRISPR/Cas9 era : a new role for Reelin in vertebrate visual sytem development Spécificité du ciblage axonale dans l'ère du CRISPR/Cas9 : un rôle nouveau pour la Reelin pendant le développement du système visuel chez les vertébrés
... versa. In this context, the structural plasticity of Robo2-defective axonal or dendritic projections allows them to target the proper synaptic partner, thereby compensating for the loss of guidance otherwise ... Voir le document complet
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Enhanced triacylglycerol production in the diatom Phaeodactylum tricornutum by inactivation of a Hotdog-fold thioesterase gene using TALEN-based targeted mutagenesis
... generated using the TAL Effector Nucleotide Targeter ...ptTES1 in an exon region in or upstream of the predicted 4HBT_2 ...ligation using the FastTALE ™ TALEN Assembly Kit (SIDANSAI ... Voir le document complet
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The Clustered, Regularly Interspaced, Short Palindromic Repeats-associated Endonuclease 9 (CRISPR/Cas9)-created
... The CRISPR/Cas9 system we used is a reprogrammed one from microbial type II CRISPR systems and has been harnessed to facilitate facile genetic manipulations in a variety of cell types and ... Voir le document complet
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CRISPR/Cas9 -mediated gene knockout of Anopheles gambiae FREP1 suppresses malaria parasite infection
... and in all tissues would therefore be expected to adversely affect ...role in blood feeding and digestion as a putative peritrophic matrix factor [ 21 ] since its inactivation could result in ... Voir le document complet
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A CRISPR–Cas9-based gene drive platform for genetic interaction analysis in Candida albicans
... saturation in LB media, and total genomic DNA was extracted from 1 ml of cultures using the PureLink Pro-96 Genomic Purification Kit (Life Technologies, ...quantified using Quant-iT High Sensitivity ... Voir le document complet
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