[PDF] Top 20 DNA polymerase epsilon is required for heterochromatin maintenance in Arabidopsis
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DNA polymerase epsilon is required for heterochromatin maintenance in Arabidopsis
... silencing in a new pol2a-12 mutant ...staining in 3-week-old L5 plants and anx2/pol2a-12 ...model for POL2A showing point ...used in this study is detailed in Table ...mutations ... Voir le document complet
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DNA polymerase epsilon is a central coordinator of heterochromatin structure and function in Arabidopsis
... disruption, DNA biosynthesis, and chromatin reassembly. Mutations in several DNA replication-related genes have been reported to destabilize silencing of transgenes and selected endogenous loci ... Voir le document complet
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DNA polymerase epsilon is a central coordinator of heterochromatin structure and function in Arabidopsis
... averaged for data ...fixed in 4% (v/v) formaldehyde 10 mM Tris-HCl for a minimum of one hour at room temperature, then rinsed in water, dried and chopped with a razor blade in 150 µl of ... Voir le document complet
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RAD50 function is essential for telomere maintenance in Arabidopsis.
... identified in yeast as being required for telomere replica- tion, such as the Cdc13 protein that binds the single-stranded portion of telomere DNA (26), as well as proteins involved in ... Voir le document complet
6
CAF-1 is essential for heterochromatin organization in pluripotent embryonic cells.
... CAF-1 is essential for heterochromatin organization in pluripotent embryonic ...changes required for embryonic gene expression, the maintenance of totipotency, and the ... Voir le document complet
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Structure and Function of Centromeric and Pericentromeric Heterochromatin in Arabidopsis thaliana
... CHROMOCENTER MAINTENANCE AND DYNAMICS Mutants impaired in factors involved in setting of epigenetic marks such as DNA methyltransferases ( Soppe et ...), in chromatin remodeling ( ... Voir le document complet
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Nucleolin is required for DNA methylation state and the expression of rRNA gene variants in Arabidopsis thaliana.
... shown in Figure 6A, PCR reactions using increased amounts of ChIP fraction detect GP (p20/p21), 59ETS (p22/p23), ITS1 (p24/p25), ITS2 (p26/p27), 39ETS (p9/p2), Sal1-2 (p28/p29) and Sal1-3 (p30/p31) rRNA gene ... Voir le document complet
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Role of the Polymerase ϵ sub-unit DPB2 in DNA replication, cell cycle regulation and DNA damage response in Arabidopsis
... of DNA damage that trig- ger replicative stress or DNA breaks, may be due to consti- tutive activation of ATR, and /or ...the DNA damage response to the growth defects caused by DPB2 over-expression, ... Voir le document complet
17
The histone variant H2A.W promotes heterochromatin accessibility for efficient DNA methylation in Arabidopsis
... Keywords Heterochromatin, H2A.W, linker histone H1, DNA methylation, chromatin accessibility Introduction Eukaryotic genomes are packaged in ...chromatin is the nucleosome, which contains a ... Voir le document complet
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Functional characterization of the DNA Polymerase epsilon and its involvement in the maintenance of genome integrity in Arabidopsis
... DISCUSSION Arabidopsis POL2A participates in cell cycle checkpoints activation and fork stabilization DNA Pol ε is required not only for DNA synthesis per se during ... Voir le document complet
243
Erasure of CpG methylation in Arabidopsis alters patterns of histone H3 methylation in heterochromatin.
... Institute for Biomedical Research, Maulbeerstrasse 66, CH-4058 Basel, Switzerland Communicated by Steven ...(received for review March 5, 2003) In mammals and plants, formation of ... Voir le document complet
6
A conserved Mcm4 motif is required for Mcm2-7 double-hexamer formation and origin DNA unwinding
... molecules in each state at the time of second hexamer binding. In the wild-type Mcm2-7 model (A), the transition from pre-DH to DH is the only process that occurs at an appreciable rate, while ... Voir le document complet
24
DNA polymerase eta is the sole contributor of A/T modifications during immunoglobulin gene hypermutation in the mouse.
... the DNA-binding moiety of the mismatch repair complex consti- tuted by the MSH2–MSH6 proteins, together with DNA polymerase 𝛈 (pol ...mutagenesis is still observed upon inactivation in ... Voir le document complet
8
Mnk1 kinase activity is required for abscission.
... MNK1 is required to avoid the formation of multinucleated ...stained for microtubules, DNA and centrosomes, and then observed under an epifluorescent ...observed in the control, whereas ... Voir le document complet
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Conserved electron donor complex Dre2-Tah18 is required for ribonucleotide reductase metallocofactor assembly and DNA synthesis
... concerns for drastically varying ββ′ levels. Manganese is known to be able to occupy the iron-binding site of class Ia RNR, resulting in catalytically inactive β 2 ...levels is to form Mn-β ... Voir le document complet
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Calsenilin is required for endocrine pancreas development in zebrafish.
... development in zebrafish has been previously described in ...cells in the developing pancreas appears earlier than calsenilin expression and strongly suggests that calsenilin is not a marker ... Voir le document complet
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BRCA1 Recruitment to Transcriptional Pause Sites Is Required for R-Loop-Driven DNA Damage Repair
... little is known about potential direct interaction between specific proteins and ...exist. For example, there could be direct recognition of an RNA:DNA hybrid, ...ssDNA. For BRCA1, it is also ... Voir le document complet
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Changes in 5S rDNA Chromatin Organization and Transcription during Heterochromatin Establishment in Arabidopsis
... rDNA in the ddm1 background (Figure 1A) was correlated with an increased proportion of mi- nor 5S rRNAs (Table 1), indicating the transcription of addi- tional 5S rRNA ...that in ddm1 ... Voir le document complet
11
The Guardian of the Genome Revisited: p53 Downregulates Genes Required for Telomere Maintenance, DNA Repair, and Centromere Structure
... mutations in genes encoding proteins of the telomerase complex (DKC1, NHP2, NOP10, TERC, TERT) or required for its assembly (WRAP53), components of the shelterin complex (ACD, TINF2) as well as other ... Voir le document complet
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ID4 Is Required for Normal Ependymal Cell Development
... important for correct timing on ependymal cell ...resulted in a decreased fraction of mature multiciliated ependymal cells (presenting multiple BB) at P10, suggesting delayed maturation compared to control ... Voir le document complet
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