Population genetic data of eight tetrameric short tandem repeats (STRs) in Casablanca resident population to use in forensic casework.

Announcement of population data

Population genetic data of eight tetrameric short tandem repeats (STRs) in Casablanca resident population

to use in forensic casework

Faiza Chbel

, Sellama Nadifi

, Cristina Martinez-Bouzas

, Said Louahlia

, Houssine Azeddoug

, Marian Martinez De Pancorbo

aLaboratoire de Ge´ne´tique Humaine, Faculte´ de Me´decine et de Pharmacie, Universite´ Hassan II, Casablanca, Morocco

bLaboratoire de BBCM, Faculte´ de Sciences, Route d’El Jadida, Universite´ Hassan II, Casablanca, Morocco

cDpto de Zoologia y Dinamica Cellular Animal, Facultad de Farmacia, Universidad del Pays Vasco, Vitoria, Spain

Received 10 September 2002; received in revised form 18 November 2002; accepted 21 November 2002

Abstract

The allele frequencies for eight short tandem repeat (STR) loci HUMvWA, HUMFES/FPS, HUMF13A, HUMF13B, HUMTHO1, HUMTPOX, HUMCSF1P0, HUMLPL included in Geneprint

STR kits were obtained from 234 unrelated individuals in Casablanca.

#

2002 Elsevier Science Ireland Ltd. All rights reserved.

Keywords: Short tandem repeat; Population data; Morocco; Geneprint^TMSTR

Population: Two hundred thirty-four unrelated and healthy volunteers resident in Casablanca (the industrial capital of Morocco where the urban growth is maintained by immigration from all parts of Morocco).

Extraction: Phenol–chloroform protocol.

PCR: The 30 ng of DNA were typed using two multiplex sets: FFv triplex and CTT triplex and two simplex sets included in Geneprint

STR kits. The amplifications were carried in ‘‘Primus MWG-Biotech’’ according to the man- ufacture’s protocol (Promega Corporation).

Typing: Four percent denatured polyacrylamide gels followed by silver staining using ladders provided in Promega Geneprint

STR kits.

Result: As shown in

Quality control: GEP-ISFG PT.

Analysis of Data: Genepop ver3.3

and Biosys-1.7 package

Access of data:

Other remarks: This population represent the general Moroccan population and was used to establish STR data- base for use in forensic casework. Previous population data

have been reported but they involved the two major ethnic groups (Berbers and Arabs). This paper follows the guidelines for publication of population data requested by the journal

Forensic Science International 132 (2003) 82–83

*Corresponding author. Tel.:þ212-2-2271630x340.

E-mail address:nadifi@fmp-uh2c.ac.ma (S. Nadifi).

0379-0738/02/$ – see front matter#2002 Elsevier Science Ireland Ltd. All rights reserved.

doi:10.1016/S0379-0738(02)00433-4

Acknowledgements

This work was supported by grants from the Morocco–

Hispanic Cooperation (24/SVR/98) and Hassan II University APR project.

References

[1] M. Raymond, F. Rousset, Genepop version 1,2: a population genetics software for exact tests and ecumenicism, J. Hered. 86 (1995) 248–249.

[2] D.L. Swofford, R.B. Selander, BIOSYS-1, A Computer Program for the Analysis of Allelic Variation in Population

Genetics an Biochemical Systematics, Release no. 1.7, University of Illinois, Urbana, IL, 1989.

[3] A. Pe´rez-Lezaun, F. Calafell, J. Clarimo´n, E. Bosh, E. Mateu, L. Gusma˜o, A. Amorim, N. Benchemsi, J. Bertranpetit, Allele frequencies of 13 short tandem repeats in population samples from the Iberian Peninsula and Northern Africa, Int. J. Leg.

Med. 113 (2000) 208–214.

[4] E. Bosch, J. Clarimo´n, A. Pe´rez-Lezaun, F. Calafell, STR data for 21 loci in northwestern Africa, Forensic Sci. Int. 116 (2001) 41–51.

[5] P. Lincoln, A. Carracedo, Publication of population data of human polymorphisms, Forensic Sci. Int. 110 (2000) 3–5.

Table 1

Allele frequencies for eight STR loci in Moroccan population

Allele F13A CSF1PO TPOX FESFPS THO1 LPL F13B VWA

N¼213 N¼230 N¼230 N¼207 N¼229 N¼234 N¼213 N¼215

3.2 0.183 – – – – – –

4 0.070 – – – – – – –

5 0.230 – – – – – –

6 0.185 – 0.017 – 0.148 – 0.254 –

7 0.268 0.007 0.013 – 0.245 0.002 0.056 –

8 0.023 0.033 0.430 0.022 0.181 0.002 0.201 –

9 0.005 0.026 0.167 0.014 0.288 0.077 0.225 –

9.3 – – – – 0.103 – – –

10 0.000 0.324 0.102 0.343 0.035 0.265 0.263 –

11 0.002 0.307 0.254 0.374 – 0.378 – –

12 0.007 0.270 0.015 0.193 – 0.233 – –

13 0.005 0.035 0.000 0.051 – 0.043 – 0.007

14 0.009 0.000 – – – – – 0.121

15 0.009 0.000 – – – – – 0.156

16 0.002 – – – – – – 0.263

17 – – – – – – – 0.249

18 – – – – – – – 0.121

19 – – – – – – – 0.060

20 – – – – – – – 0.023

Ho 0.704 0.743 0.717 0.773 0.799 0.743 0.777 0.819

He 0.804 0.725 0.712 0.703 0.792 0.725 0.773 0.813

PD 0.931 0.871 0.859 0.850 0.923 0.871 0.909 0.949

CE 0.607 0.478 0.475 0.447 0.588 0.478 0.547 0.627

P 0.005 0.596 0.200 0.233 0.720 0.066 0.263 0.239

Ho, observed heterozygosity; He, expected heterozygosity; PD, power of discrimination; CE, a priori chance of exclusion;P, Hardy–Weinberg equilibrium exact test based on 1000 shufflings.

F. Chbel et al. / Forensic Science International 132 (2003) 82–83 83