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Variability of cotton diploid species SSR amplification in interspecific hybrids

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Reference : PAG-XIII-P127

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Reference Title Variability Of Cotton Diploid Species SSR Amplification In Interspecific Hybrids Journal Abstract of Plant and Animal Genome Conference, San

Diego, CA, USA Journal_code PAG Year 2005 URL http://www.intl-pag.org/PAGarchives.html Author Konan ON Baudoin JP Benbouza H Lacape JM Mergeai G

Abstract A total of 206 mapped SSR markers distributed on the 26 linkage groups of the most recent Gossypium hirsutum genetic

maps were used to monitor the introgression of diploid species chromosome fragments in the framework of three

different breeding programmes involving the G. hirsutum x G. raimondii x G. sturtianum (HRS), G. hirsutum x G. longicalyx

(HL) and G. hirsutum x G. australe (HA) interspecific hybrids. In these three hybrids, the rate of reduction of

the diploid parent specific SSR numbers were respectively 31

% for G. longicalyx, 38 % for G. sturtianum, 46 % for G. raimondii and 42 % for G. australe while the number of

specific G. hirsutum SSRs remained almost unchanged. An important part of this variability in the amplification of

the diploid species specific SSR alleles in the interspecific hybrids can be explained by the high level of

heterozygosity observed in the different parent species which was respectively 94.4 % for G. longicalyx, 62.1 % for

G. sturtianum, 65.5 % for G. raimondii but only 12 % for G. australe . The possible influence of other factors mentioned

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