Transcriptome profiling
Simultaneous miRNA and mRNA transcriptome profiling of human myoblasts reveals a novel set of myogenic differentiation-associated miRNAs and their target genes.
... Transcriptome profiling and data processing Human primary myoblasts were sacrificed directly on plates at 30% confluency using Trizol, RNA was prepared using organic extraction and ethanol precipitation as ...
21
Transcriptome profiling of the Olig2-expressing astrocyte subtype reveals their unique molecular signature
... the transcriptome profiling A: Schematic of the experimental procedure: dissociation and purification of glial cell populations from double transgenic reporter mouse, followed by RNA-seq and ...
38
Transcriptome profiling of Chironomus kiinensis under phenol stress using Solexa sequencing technology
... Str2, Str1, Npra11 and Npra16. In addition, phenol affected the expression of four genes encoding class B receptors, including Relar2, Calcr, Oxr2 and Dhr. Most genes encoding receptors that were affected by phenol were ...
13
Transcriptome profiling of laser-captured crown root primordia reveals new pathways activated during early stages of crown root formation in rice
... * pascal.gantet@umontpellier.fr Abstract Crown roots constitute the main part of the rice root system. Several key genes involved in crown root initiation and development have been identified by functional genomics ...
16
Transcriptome Profiling in Rat Inbred Strains and Experimental Cross Reveals Discrepant Genetic Architecture of Genome-Wide Gene Expression
... pathways and networks (Ghazalpour et al. 2006; Chen et al. 2008; Emilsson et al. 2008; Monti et al. 2008). The regulation of gene expres- sion is orchestrated through complex local (cis-mediated) and distant (mediated in ...
14
Comparative Transcriptome Profiling of Virulent and Attenuated Ehrlichia ruminantium Strains Highlighted Strong Regulation of map1- and Metabolism Related Genes
... global transcriptome profile of two distant virulent strains of ...the transcriptome profiles obtained between the different replicates were at least 72% identical (main linear correlation coefficient), ...
17
Transcriptome profiling of a TGF-beta-induced epithelial-to-mesenchymal transition reveals extracellular clusterin as a target for therapeutic antibodies
... 3 Computational Chemistry and Biology Group, Biotechnology Research Institute, National Research Council of Canada, Montre´al, Quebec, Canada; 4 Animal Cell Culture Technology Group, Biotechnology Research Institute, ...
15
Transcriptome Profiling and Genetic Study Reveal Amplified Carboxylesterase Genes Implicated in Temephos Resistance, in the Asian Tiger Mosquito Aedes albopictus
... Assembly. The de novo assembly was performed on the pre-processed (singleton and re- paired) reads of combined libraries using Trinity package [ 23 ] Release: 2013-11-10 with default parameters and the minimum contig ...
18
Transcriptome profiling identifies candidate genes associated with the accumulation of distinct sulfur γ-glutamyl dipeptides in Phaseolus vulgaris and Vigna mungo seeds
... profiled on a 454 Genome Sequencer-FLX Titanium platform. A half-plate was sequenced for each sample starting from the same amount of input cDNA. Table 1 lists general statistics for the 454 transcriptome data. ...
13
Transcriptome profiling of sorted endoreduplicated nuclei from tomato fruits: how the global shift in expression ascribed to DNA ploidy influences RNA-Seq data normalization and interpretation
... the transcriptome of the largest cells (of high ploidy levels) at this developmental stage has already changed when compared to smaller cells (of low ploidy ...
26
Transcriptome profiling of mouse samples using nanopore sequencing of cDNA and RNA molecules
... (ONT) company commercially released a portable sequencer which is able to sequence very long DNA frag- ments 3 and enable now the sequencing of complex genomes 4 – 6 . Moreover this device (namely MinION) is also able to ...
13
Comprehensive transcriptome profiling of root-knot nematodes during plant infection and characterisation of species specific trait
... 98 ABSTRACT The root-knot nematode species Meloidogyne enterolobii (syn. M. mayaguensis) represent a new threat for global agriculture due to its wide host range and its capacity to reproduce on commercial plant ...
177
Comparative Transcriptome Profiling of Resistant and Susceptible Sugarcane Cultivars in Response to Infection by Xanthomonas albilineans
... comparative transcriptome analysis between resistant (LCP 85-384) and susceptible (ROC20) sugarcane cultivars infected by ...the transcriptome changes in sugarcane in response to infection by ...
21
Transcriptome profiling of the honeybee parasite Varroa destructor provides new biological insights into the mite adult life cycle
... Transcriptome sequencing RNA-seq libraries were constructed with the Truseq stranded mRNA sample preparation (Low throughput protocol) kit from Illumina (San Diego, CA, USA). Two hundred nanograms of total RNA ...
20
Structure, dynamique et évolution du transcriptome chez les conifères
... 2.2.2 Results An oligonucleotide microarray was developed from Picea glauca and P. sitchensis cDNA datasets. It represents 23,853 unique genes and was shown to be suitable for transcriptome profiling in ...
173
Étude de la variabilité inter-individuelle du transcriptome soumis à un stimulus
... L’analyse du transcriptome est un bon exemple de ces évolutions. Avec l’avènement de la technique de PCR (Mullis and Faloona, 1987), les premières analyses se concentraient péniblement sur une dizaine de gènes ...
201
Approches intégrées du génome et du transcriptome dans les maladies complexes humaines
... dans la compréhension des mécanismes liant ces loci aux maladies complexes. Cette analyse a également permis de mettre en évidence l'existence de contamination par des types cellulaires non désirés dans l'étude GHS. Nous ...
257
De Novo sequencing and transcriptome analysis for Tetramorium bicarinatum: a comprehensive venom gland transcriptome analysis from an ant species
... Figure 10 Amino acid sequence alignment of venom serine protease (VSP) from Hymenopteran species.. The predicted VSP from T.[r] ...
19
Profiling novel high-conductivity 2D semiconductors
... There are different ways to maximize the velocity con- tribution ii); in general, one needs to maximize the ve- locity in the direction of transport. In the case of InSe, the velocity is[r] ...
13
Gene expression profiling in sinonasal adenocarcinoma.
... Immunohistochemical analysis of LGALS4, ACS5 and CLU To confirm the variation in expression of the selected genes at the protein level, we performed immunohisto- chemical analysis of mat[r] ...
13