Specific cell capture
Antibody-functionalized nanoporous surfaces enable high throughput specific cell capture
... Sample processing at high flow rates poses three critical challenges: (1) Decreased transport of cells to the reactive surface (2) High shear stress and decreased interaction [r] ...
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Bioengineered surfaces and hydrogels for specific cell capture and release from whole blood
... These approaches have shown the ability to isolate rare cell populations while minimizing non-specific capture; also, a label free approach significantly reduces the [r] ...
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Antibody-Functionalized Fluid-Permeable Surfaces for Rolling Cell Capture at High Flow Rates
... strong, specific biomolecular bond formation by slowing down cell rolling across the ...stabilize cell motion against the local shear ...enhanced cell-surface interaction allows ...
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Comment on “Diversification of the antigen-specific T cell receptor repertoire after varicella zoster vaccination”
... of cell division by co-dilution of CFSE and CTV reveals the unexpected presence of CFSE+ CTV+ CD3+ ...The cell mixture was analyzed by flow cytometry before (left panel) and after 6 days of in vitro ...
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PD-1 expression conditions T cell avidity within an antigen-specific repertoire
... of specific Vß subfamilies amplified in the control condition (dotted lines) or in the presence of anti-PD-1 Ab (solid lines) were evaluated by measuring IFN- (left panel), TNF- production (middle panel) and ...
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Cell-specific prediction and application of drug-induced gene expression profiles
... leverage cell-specific responses has been hindered by limited data availability across cell types 6, 7 ...of cell types. However, this combinatorial space of drugs and cell types is ...
13
SpiCee: A Genetic Tool for Subcellular and Cell-Specific Calcium Manipulation
... measurements. Cell death assay HEK293 Cells were plated on poly-lysine-coated coverslips and transfected the following day with a pCX-mRFP or a pCX-SpiCee vector using Lipofectamine 2000 (Thermo Fisher) following ...
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Cytokine-capture nanoparticles to quantify IL-2 concentrations within the immunological synapse during T cell activation
... Although there are patents that claim the use of nanoparticles as a substrate to present multiple p-MHC molecules, the cytokine capture device still has the advantage of q[r] ...
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Genotyping by sequencing using specific allelic capture to build a high-density genetic map of durum wheat
... few specific durum wheat tools are currently ...polymorphisms specific to wild and ancient germplasm, such as the wild ...parental specific polymorphism may represent a valuable alternative to high ...
21
Cell type–specific genetic and optogenetic tools reveal hippocampal CA2 circuits
... CA2- specific Cre knock-in mouse (MAP3K15 Cre) with a Cre-dependent virus, AAV9-EF1α-DIO- ChR2-YFP ...high cell type specificity of the knock-in mouse (PCP4/YFP ...
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AIRE polymorphism, melanoma antigen-specific T cell immunity, and susceptibility to melanoma
... T cell response because MAGE antigens are AIRE-dependent antigens expressed by mTECs [17-21] and have been suggested to be tumor rejection antigens [22, ...T cell survival and in vivo susceptibility to ...
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Heterocyst formation under the control of a cell-specific antisense RNA
... heterocyst- specific asRNA, as_glpX, antisense to the glpX gene encoding the Calvin cycle enzyme sedoheptulose-1,7-biphosphatase/fructose-1,6-biphosphatase (SBPase) involved in CO 2 ...
4
Alkyladenine DNA glycosylase (Aag)-dependent cell-specific responses to alkylating agents
... To our knowledge, this is the first documented case where both loss and enhancement of DNA repair initiation has caused increased cell sensitivity to DNA damage co[r] ...
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Compartment and cell-type specific hypoxia responses in the developing Drosophila brain
... of cell bodies (Fig. 1D). On the side of the central brain we found cell bodies with a relatively large cytoplasm containing abundant mitochondria, endoplasmic reticulum, ribosomes and other organelles, as ...
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Cell type–specific mRNA purification by translating ribosome affinity purification (TRAP)
... new cell type, one would expect to see a halving of TRAP yield with half the amount of matrix used in TRAP purifications -- and no detectable purification of RNA from the non- TRAP control with either ...by ...
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Antigen-specific B-cell receptor sensitizes B cells to infection by influenza virus
... In vivo influenza infections 8-10 week old male mice were anesthetized by a single dose of avertin (1.25% tribromoethanol, GIBCO), 250 mg/kg body weight, delivered by intraperitoneal injection. A/WSN/33 (2×10ˆ 5 ...
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Crowd Motion Capture
... Crowds of people exhibit particular and subtle behaviors whose complexity reflects the complex nature of human be- ings. Animating crowds are usually a tedious task for an- imators who rely most of the time on simulation ...
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TOPAZ1, a novel germ cell-specific expressed gene conserved during evolution across vertebrates
... line- specific using two different approaches; the enrichment of mouse germ cells by SSEA1 antibody and the use of gonads from WlacZ/ WlacZ mutant mice devoid of germ ...
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Cell-Type-Specific Synchronization of Neural Activity in FEF with V4 during Attention
... by cell class, the results showed that visual, visuomovement, and movement neurons display distinct FEF-V4 coherence ...FEF cell classes (Kruskal-Wallis, p < ...
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Bisquinolinium compounds induce quadruplex-specific transcriptome changes in HeLa S3 cell lines.
... antibody specific to G-quadruplex motifs, Fernando ...HGC-27 cell line. It should be noted that the choice of the cell line has very likely a strong impact on the outcome of whole-transcrip- tome ...
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