Table S1. Patient characteristics for tumor RNAseq analysis
Variable Poor prognosis
(OS<24mo, n=9)
Good prognosis
(OS≥24mo, n=10) p-value
Patient variables
Median age at diagnosis (range) Sex
Female Male
55 (34, 73) 2 7
59 (23, 73) 3 7
0.9 0.7
Tumour variables Grade
II III
IV Histology
Astrocytoma Oligodendroglioma
GBM
MGMT gene promoter status Unmethylated Methylated Unknown IDH gene status
Mutant Wildtype
0 0 9 0 0 9 8 0 1 0 9
0 3 7 3 0 7 6 3 1 2 7
0.07
0.07
0.07
0.1
Table S2. Patient characteristics for tumor flow-cytometry analysis
Variable Poor prognosis
(OS<24mo, n=19)
Good prognosis
(OS≥24mo, n=20) p-value
Patient variables
Median age at diagnosis (range) Sex
Female Male
59 (40, 81) 7 12
40 (23, 68) 9 11
<0.0001 0.6
Tumour variables Grade
II III
IV Histology
Astrocytoma Oligodendroglioma
GBM
MGMT gene promoter status Unmethylated Methylated Unknown IDH gene status
Mutant Wildtype
2 3 14
4 1 14 12 3 4 15
2
12 5 3 9 8 3 5 5 10
5 13
0.0006
0.0007
0.06
0.002
Table S3. Patient characteristics for PBMC RNAseq analysis
Variable Poor prognosis
(OS<21mo, n=14)
Good prognosis
(OS≥21mo, n=16) p-value
Patient variables
Median age at diagnosis (range) Sex
Female Male
57 (34, 73) 5 9
57 (23, 73) 6 10
>0.999 0.9
Tumour variables Grade
II III
IV Histology
Astrocytoma Oligodendroglioma
GBM
MGMT gene promoter status Unmethylated Methylated Unknown IDH gene status
Mutant Wildtype
0 0 14
0 0 14 11 0 3 10
0
0 3 13
0 3 13
7 7 2 8 3
0.09
0.09
0.02
0.2
Table S4. TCGA patient characteristics.
Variable High expression
(n=334)
Low expression
(n= 335) p-value Patient variables
Median age at diagnosis (range) 52 (37.5, 62) 42 (33,54) <0.001 Tumour variables
Initial diagnosis, n Grade
II III
IV
Unknown
Histology
Astrocytoma Oligoastrocytoma
Oligodendroglioma GBM
Unknown Subtype
Classical Mesenchymal
Neural Proneural Unknown
MGMT gene promoter status, n Unmethylated
Methylated Unknown IDH gene status, n
Mutant Wildtype
68 128 117 21
111 52 51 120
0
67 93 40 72 62
114 194 26
143 185
158 116 33 28
83 78 140
32 2
19 3 71 166
76
47 283
5
286 48
<0.001
<0.001
<0.001
<0.001
<0.001
Table S5. Patient characteristics for ELISA analysis
Variable Poor prognosis
(OS<24mo, n=20)
Good prognosis
(OS≥24mo, n=20) p-value
Patient variables
Median age at diagnosis (range) Sex
Female Male
59 (32, 81) 6 14
38 (23, 68) 9 11
<0.0001 0.5
Tumour variables Grade
II III
IV Histology
Astrocytoma Oligodendroglioma
GBM
MGMT gene promoter status Unmethylated Methylated Unknown IDH gene status
Mutant Wildtype
2 4 14
5 1 14
6 2 12
2 2
12 5 3 10
7 3 1 1 18
2 0
0.0008
0.001
0.08
0.3
