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Mesenchymal Stem Cells and Endothelial Progenitor Cells: Involvement of Matrix Metalloproteinase-14
Mariusz Malinowski, Katarzyna Pietraszek, Corinne Perreau, Mateusz Boguslawski, Veronique Decot, Jean-François Stoltz, Laurent Vallar, Jolanta
Niewiarowska, Czeslaw Cierniewski, François-Xavier Maquart, et al.
To cite this version:
Mariusz Malinowski, Katarzyna Pietraszek, Corinne Perreau, Mateusz Boguslawski, Veronique Decot,
et al.. Effect of Lumican on the Migration of Human Mesenchymal Stem Cells and Endothelial
Progenitor Cells: Involvement of Matrix Metalloproteinase-14. PLoS ONE, Public Library of Science,
2012, 7 (12), �10.1371/journal.pone.0050709�. �hal-01777758�
Mesenchymal Stem Cells and Endothelial Progenitor Cells: Involvement of Matrix Metalloproteinase-14
Mariusz Malinowski
1,2., Katarzyna Pietraszek
1., Corinne Perreau
1, Mateusz Boguslawski
1,
Ve´ronique Decot
3, Jean-Franc¸ois Stoltz
3, Laurent Vallar
4, Jolanta Niewiarowska
5, Czeslaw Cierniewski
2, Franc¸ois-Xavier Maquart
1,6, Yanusz Wegrowski
1, Ste´phane Bre´zillon
1*
1
Laboratoire de Biochimie Me´dicale et de Biologie Mole´culaire, CNRS FRE 3481, Universite´ de Reims Champagne-Ardenne, Reims, France,
2Institute of Medical Biology, Polish Academy of Sciences, Lodz, Poland,
3Unite´ de The´rapie Cellulaire et banque de Tissus, CHU Brabois, CNRS UMR 7561, Faculte´ de Me´decine, Vandoeuvre Le`s Nancy, France,
4Microarray Center, CRP-Sante´, Luxembourg, Luxembourg,
5Department of Molecular and Medical Biophysics, Medical University of Lodz, Lodz, Poland,
6CHU de Reims, Reims, France
Abstract
Background: Increasing number of evidence shows that soluble factors and extracellular matrix (ECM) components provide an optimal microenvironment controlling human bone marrow mesenchymal stem cell (MSC) functions. Successful in vivo administration of stem cells lies in their ability to migrate through ECM barriers and to differentiate along tissue-specific lineages, including endothelium. Lumican, a protein of the small leucine-rich proteoglycan (SLRP) family, was shown to impede cell migration and angiogenesis. The aim of the present study was to analyze the role of lumican in the control of MSC migration and transition to functional endothelial progenitor cell (EPC).
Methodology/Principal Findings: Lumican inhibited tube-like structures formation on MatrigelH by MSC, but not EPC. Since matrix metalloproteinases (MMPs), in particular MMP-14, play an important role in remodelling of ECM and enhancing cell migration, their expression and activity were investigated in the cells grown on different ECM substrata. Lumican down- regulated the MMP-14 expression and activity in MSC, but not in EPC. Lumican inhibited MSC, but not EPC migration and invasion. The inhibition of MSC migration and invasion by lumican was reversed by MMP-14 overexpression.
Conclusion/Significance: Altogether, our results suggest that lumican inhibits MSC tube-like structure formation and migration via mechanisms that involve a decrease of MMP-14 expression and activity.
Citation:
Malinowski M, Pietraszek K, Perreau C, Boguslawski M, Decot V, et al. (2012) Effect of Lumican on the Migration of Human Mesenchymal Stem Cells and Endothelial Progenitor Cells: Involvement of Matrix Metalloproteinase-14. PLoS ONE 7(12): e50709. doi:10.1371/journal.pone.0050709
Editor:
Nikos K. Karamanos, University of Patras, Greece
Received
June 4, 2012;
AcceptedOctober 24, 2012;
PublishedDecember 7, 2012
Copyright:ß
2012 Malinowski et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding:
The authors thank the INCa-CNRS-Polish Academy of Sciences program, Institut National du Cancer (INCa) (ACI Canceropoˆles 2007–2009), the Re´gion Champagne-Ardenne, the Fonds Europe´en de De´veloppement Re´gional (FEDER) (contrat de projet E´tat-re´gion (CPER),CPER 2007–2013) and the Ligue Nationale contre le Cancer (comite´ de la Marne) for their financial support. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing Interests: