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In vivo function of the lipid raft protein flotillin-1 during cd8+ t cell–mediated host surveillance

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Academic year: 2021

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1 Supplemental Figure Legend

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Supplemental Figure 1. Flot1 localization at uropod of polarized T cell blasts. Phase contrast and 4

immunofluorescent images of polarized activated WT and Flot1-/- T cell blasts stimulated with CCL19 5

and stained for Flot1. Scale bar, 10 µm. 6

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Supplemental Figure 2. Flot2 deficiency and chemotaxis. A. Expression of Flot1 and Flot2 in 10

splenocytes isolated from WT and Flot2-/- mice. B. Chemotaxis of naïve WT and Flot2-/- splenocytes 11

towards 100 nM CCL21 through 3 and 5 µm filter inserts. Shown is mean ± SD of two independent 12

experiments performed in triplicates. 13

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Supplemental Figure 3. Recovery of OT-I and Flot1-/- OT-I T cells on > day 28 after HSV TOM-OVA

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infection. A. Ratio of Flot1-/- OT-I to OT-I T cells analyzed by flow cytometry in spleen (Spl), draining 18

inguinal and axillary lymph nodes (dLN) and tattooed skin. B. Ratio of % OT-I or Flot1-/- OT-I CD8+ T 19

cells in skin versus dLN of the same recipient determined by flow cytometry. Data in A and B are 20

pooled from 2 independent experiments (n = 5-8). 21

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