Histone variant H2ABbd confers lower stability to the nucleosome.

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Submitted on 28 Oct 2008

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Histone variant H2ABbd confers lower stability to the

nucleosome.

Thierry Gautier, D Wade Abbott, Annie Molla, Andre Verdel, Juan Ausio,

Stefan Dimitrov

To cite this version:

Thierry Gautier, D Wade Abbott, Annie Molla, Andre Verdel, Juan Ausio, et al.. Histone variant H2ABbd confers lower stability to the nucleosome.. EMBO Reports, EMBO Press, 2004, 5 (7), pp.715-20. �10.1038/sj.embor.7400182�. �inserm-00335082�

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A

B

131-2

1 2 3 4

_{1 2 3 4 5 6}

H1 H2A H3 H2B H4 H5

H2A-Bbd

N

D

395 345 332-7 270 259 206 190 174 151-3 141

Fig.1

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NaCl Mitosis GFP-H2A GFP-H2ABbd Interphase 60 80 100 0 0.2 0.4 0.6 0.8 0.15 0.2 0.4 Control 60 80 100 GFP-H2A GFP-H2ABbd 0.6 M 0 0.2 0.4 0.6 0.8 M

Fig.3

B

C

H2B H2A H3 H2A Bbd H4 154 bp 3 1 2

A

D

E

H2A GFP-fusions

1 2

F

G

Anti-H2A antibody H2A GFP-H2A % of F lu or es ce n ce

G

F

P

-H

2A

G

F

P

-H

2A

Bb

d

Int erpha se M it os is Int erpha se M it os is % of F lu or es ce n ce Anti-GFP antibody 1 2 45 kD NaCl

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30 min pre-bleach 0 2 4 6 10 15

Fig.4

A

B

GFP-H2A GFP-H2ABbd % of fl u or es ce n ce r ec ove ry