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Genomic determinants of the efficiency of internal ribosomal entry sites of viral and cellular origin

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Academic year: 2021

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Table 1. Plasmids used in this study: final (in bold) and intermediate constructs
Figure 1. IRES activity in cell lines of diverse origins. IRES activity was estimated as the ratio between eGFP expression (second cistron) and mRFP (first cistron) in the transduced population and normalized by values from mock transduced cells
Figure 2. GFP expression requires an intact XIAP IRES. (A) PCR amplification using primers SG-1667 and SG-1669 on DNA constructs containing an intact XIAP IRES or a spliced XIAP IRES
Figure 3. EMCV and XIAP IRES activity. Distribution of IRES activ- activ-ity values for EMCV (A), and XIAP (B) in transduced CEPH cell lines.

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