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AN EELS STUDY OF THE INTRANUCLEAR INCLUSIONS OF THE EPIDIDYMIS PRINCIPAL CELLS IN THE GARDEN DORMOUSE, ELIOMYS
QUERCINUS L.
F. Hawkes, Yolande Kihn, Jean Sévely
To cite this version:
F. Hawkes, Yolande Kihn, Jean Sévely. AN EELS STUDY OF THE INTRANUCLEAR IN- CLUSIONS OF THE EPIDIDYMIS PRINCIPAL CELLS IN THE GARDEN DORMOUSE, ELIOMYS QUERCINUS L.. Journal de Physique Colloques, 1984, 45 (C2), pp.C2-585-C2-586.
�10.1051/jphyscol:19842134�. �jpa-00223804�
JOURNAL DE PHYSIQUE
Colloque C2, supplément au n°2, Tome 45, février 1984 page C2-585
AN EELS STUDY OF THE INTRANUCLEAR INCLUSIONS OF THE EPIDIDYMIS PRINCIPAL CELLS I N THE GARDEN DORMOUSE, ELIOMYS QUERCINUS L .
F . Hawkes, Y. Kihn and J . S e v e l y
Laboratoire d'Optique Eleatronique du CNRS , 29 rue Jeanne Marvig, 31400 Toulouse, France
Résumé : Les inclusions intranucléaires des cellules principales de l'ëpididyme du l ë - rot contiennent C, N, 0, Mg, P, S et Fe.
A b s t r a c t : In the i n t r a n u c l e a r i n c l u s i o n s of the epididymis p r i n c i p a l c e l l s of the garden dormouse, C, N, 0 , Mg, P, S and Fe are found.
Hammar was the f i r s t t o r e p o r t , i n 1897, g l o b u l a r i n c l u s i o n s i n the n u c l e i of the co- lumnar e p i t h e l i a l c e l l s l i n i n g the ductus e p i d i d y m i d i s . He observed them i n the dog where they are very abundant. They are also present i n man and i n the s t a l l i o n but are absent from sheep, c a t , r a b b i t guinea p i g , r a t and mouse. We have now found them i n an a s c r o t a l h i b e r n a t o r , the garden dormouse, Etiomy-i queJiciinuA L., but we have been unable t o detect them i n another h i b e r n a t o r , t h i s time a s c r o t a l mammal, the t h i r t e e n - l i n e d ground q u i r r e l SpexmophiluA t>U.de.cejnlLnejCLtu6.
Bp^didyrnli, pMJ.ncJ.pal cell. The nu.cZe.aA. <LncluAlonA {cvoiow) a/ie globulin, x $,000.
associé à l'Université Paul Sabatier
Article published online by EDP Sciences and available at http://dx.doi.org/10.1051/jphyscol:19842134
C2-586 JOURNAL DE PHYSIQUE
The granules are found i n the p e r i n u c l e a r space before t h e i r passage i n t o t h e nucleus I t seems t h a t the endoplasmic r e t i c u l u m sacculae which stock them a f t e r t h e i r syn- t h e s i s , fuse w i t h the o u t e r nuclear envelope. Indeed, many o f our observations have shown a c o n t i n u i t y between t h e c i s t e r n a l lumen and t h e p e r i n u c l e a r space. The granule are then i n v e s t e d by t h e i n n e r membrane o f t h e nuclear envelope and t r a n s f e r r e d i n t o the nucleus. They l a t e r d i s i n t e g r a t e i n t o a f i n e l y p a r t i c u l a t e m a t e r i a l and t h e vacu- o l e i s f i n a l l y l e f t w i t h an e l e c t r o n transparent content.
The number o f i n t r a n u c l e a r i n c l u s i o n s v a r i e s w i t h the hormonal s t a t e o f t h e animal.
A c a r e f u l study over f o u r years i n which several animals were examined each month leads us t o conclude t h a t the granules are always present j u s t before a r i s e i n p l a s - ma t e s t o s t e r o n e ; they a r e found i n January, June and September.
hNa
\ Nuclear lnclus~on
EELS a r ~ d y n h 06 a nudeatr i n d u n i o n .
OuAide. od ;the.
nudeatr i n d u n i o n .
The EELS analyses were performed w i t h an R - f i l t e r f i t t e d t o t h e Toulouse 1.2 P1V mi- croscope. We worked a t l MV. The s e l e c t e d areas were about 100 nm i n diameter and we were, t h e r e f o r e , always a n a l y s i n g o n l y the granules. The spectra were t r a c e d w i t h a Tracor TN-11. We detected C, N, 0 ; Mg was i d e n t i f i e d by i t s K l i n e a t 1305 eV, P by t h a t a t 2140 eV, S by t h a t a t 2470 eV ; Fe was i d e n t i f i e d by i t s L2g l i n e a t 730 eV.
T h i s suggests t h a t the granules are made o f p r o t e i n associated w i t h phosphorus, s u l - phur, i r o n and magnesi urn.
