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fig|500638.3.peg.1695 [Edwardsiella tarda ATCC 23685]

fig|521000.6.peg.1201 [Providencia rettgeri DSM 1131] fig|500637.6.peg.6692 [Providencia rustigianii DSM 4541] fig|500637.6.peg.398 [Providencia rustigianii DSM 4541] 0.864

fig|471881.3.peg.851 [Proteus penneri ATCC 35198] fig|471881.3.peg.853 [Proteus penneri ATCC 35198]

fig|471881.3.peg.852 [Proteus penneri ATCC 35198] fig|1125694.3.peg.2025 [Proteus mirabilis WGLW6]

0.400 0.271 0.996 0.984

fig|566551.4.peg.215 [Cedecea davisae DSM 4568] fig|500639.8.peg.3742 [Enterobacter cancerogenus ATCC 35316] fig|718254.4.peg.2634 [Enterobacter cloacae subsp. cloacae NCTC 9394]

fig|749528.3.peg.4315 [Escherichia coli MS 45-1] fig|469595.3.peg.2949 [Citrobacter sp. 30_2]

fig|500640.5.peg.3814 [Citrobacter youngae ATCC 29220]

fig|1002368.3.peg.1538 [Yokenella regensburgei ATCC 43003] fig|431946.3.peg.3108 [Escherichia coli SE15]

fig|469598.5.peg.1076 [Escherichia sp. 3_2_53FAA] fig|364106.8.peg.3534 [Escherichia coli UTI89] fig|749546.3.peg.2089 [Escherichia coli MS 185-1] fig|749528.3.peg.4102 [Escherichia coli MS 45-1]

fig|749550.3.peg.1060 [Escherichia coli MS 200-1] fig|749531.3.peg.2560 [Escherichia coli MS 69-1] fig|749527.3.peg.2786 [Escherichia coli MS 21-1] fig|749540.3.peg.1424 [Escherichia coli MS 146-1] fig|679206.4.peg.1517 [Escherichia coli MS 119-7] fig|749538.3.peg.992 [Escherichia coli MS 116-1] fig|457401.3.peg.3050 [Escherichia sp. 4_1_40B]

fig|511145.12.peg.3231 [Escherichia coli str. K-12 substr. MG1655] fig|679202.3.peg.1018 [Escherichia coli MS 85-1]

fig|749533.3.peg.3484 [Escherichia coli MS 84-1] fig|749537.3.peg.492 [Escherichia coli MS 115-1] fig|679205.4.peg.352 [Escherichia coli MS 124-1] fig|749544.3.peg.763 [Escherichia coli MS 175-1] fig|749548.3.peg.2786 [Escherichia coli MS 196-1]

fig|386585.9.peg.4192 [Escherichia coli O157_H7 str. Sakai] fig|749547.3.peg.1093 [Escherichia coli MS 187-1]

fig|796392.4.peg.1903 [Escherichia coli MS 79-10] fig|749549.3.peg.1972 [Escherichia coli MS 198-1] fig|1127356.4.peg.2227 [Escherichia coli 4_1_47FAA] fig|749532.3.peg.1631 [Escherichia coli MS 78-1] fig|749545.3.peg.3372 [Escherichia coli MS 182-1] fig|679204.3.peg.871 [Escherichia coli MS 145-7] fig|409438.11.peg.3591 [Escherichia coli SE11] fig|679207.4.peg.2598 [Escherichia coli MS 107-1] 0.905 0.725 0.726 0.346 0.932 1.000 0.943

fig|471874.6.peg.3061 [Providencia stuartii ATCC 25827]

fig|500638.3.peg.2062 [Edwardsiella tarda ATCC 23685] fig|469613.3.peg.33 [Enterobacteriaceae bacterium 9_2_54FAA] fig|1002364.3.peg.907 [Hafnia alvei ATCC 51873]

0.898 0.994

0.873

fig|910310.3.peg.1114 [Turicibacter sp. HGF1] fig|702450.3.peg.369 [Turicibacter sp. PC909]

fig|457396.3.peg.2040 [Clostridium sp. 7_2_43FAA] fig|742739.3.peg.2682 [Clostridium perfringens WAL-14572] 1.000

fig|469609.3.peg.62 [Streptococcus sp. 2_1_36FAA]

fig|1203590.3.peg.1441 [Streptococcus sp. HPH0090] fig|742820.3.peg.1750 [Streptococcus anginosus 1_2_62CV] 0.188

1.000

fig|525258.3.peg.509 [Clostridium difficile NAP07] fig|645462.3.peg.3385 [Clostridium difficile CD196] fig|525259.3.peg.3482 [Clostridium difficile NAP08]

fig|411903.6.peg.2052 [Collinsella aerofaciens ATCC 25986] fig|742742.3.peg.744 [Collinsella tanakaei YIT 12063]

fig|521003.7.peg.400 [Collinsella intestinalis DSM 13280] fig|445975.6.peg.134 [Collinsella stercoris DSM 13279] 0.832

1.000

fig|742813.4.peg.1807 [Enterococcus saccharolyticus 30_1] fig|749516.3.peg.1833 [Enterococcus faecalis TX1346]

fig|491074.3.peg.440 [Enterococcus faecalis TX0104] fig|749491.3.peg.309 [Enterococcus faecalis TX2137] fig|749513.3.peg.2419 [Enterococcus faecalis TX1302] fig|657310.3.peg.244 [Enterococcus sp. 7L76] fig|749514.3.peg.1915 [Enterococcus faecalis TX1341] fig|749494.3.peg.1655 [Enterococcus faecalis TX4244] fig|749515.3.peg.2522 [Enterococcus faecalis TX1342] fig|749518.3.peg.1250 [Enterococcus faecalis TX2134] fig|525278.3.peg.294 [Enterococcus faecalis TX1322] 0.969

fig|665945.3.peg.855 [Lactobacillus sp. 7_1_47FAA] fig|324831.13.peg.114 [Lactobacillus gasseri ATCC 33323]

fig|257314.6.peg.126 [Lactobacillus johnsonii NCC 533] 1.000

fig|862514.3.peg.1197 [Pediococcus acidilactici DSM 20284] fig|563194.3.peg.193 [Pediococcus acidilactici 7_4]

fig|568703.30.peg.334 [Lactobacillus rhamnosus GG] fig|1002365.5.peg.425 [Lactobacillus rhamnosus ATCC 21052] fig|525361.3.peg.1784 [Lactobacillus rhamnosus LMS2-1] fig|543734.4.peg.274 [Lactobacillus casei BL23]

fig|537973.8.peg.2331 [Lactobacillus paracasei subsp. paracasei 8700_2] fig|525337.3.peg.1053 [Lactobacillus paracasei subsp. paracasei ATCC 25302] 0.687 0.868 0.632 0.872 0.905 0.998 0.538

fig|469616.3.peg.507 [Fusobacterium mortiferum ATCC 9817] fig|469618.3.peg.1211 [Fusobacterium varium ATCC 27725] fig|469617.3.peg.3037 [Fusobacterium ulcerans ATCC 49185] fig|457404.3.peg.3262 [Fusobacterium sp. 12_1B]

0.571 0.997 0.464

fig|718252.3.peg.1294 [Faecalibacterium prausnitzii L2-6] fig|428126.7.peg.725 [Clostridium spiroforme DSM 1552]

fig|545697.3.peg.578 [Clostridium celatum DSM 1785] fig|1203606.4.peg.2198 [Butyricicoccus pullicaecorum 1.2]

fig|658081.3.peg.269 [Lachnospiraceae bacterium 1_1_57FAA] fig|665950.3.peg.313 [Lachnospiraceae bacterium 3_1_46FAA] fig|665951.3.peg.523 [Lachnospiraceae bacterium 8_1_57FAA] fig|411460.6.peg.609 [Ruminococcus torques ATCC 27756] 0.915

0.708 0.802 0.977

fig|518637.5.peg.21 [Eubacterium biforme DSM 3989]

fig|428127.7.peg.825 [Eubacterium dolichum DSM 3991] fig|658659.3.peg.3323 [Erysipelotrichaceae bacterium 3_1_53]

fig|457422.3.peg.2843 [Erysipelotrichaceae bacterium 2_2_44A] fig|469614.3.peg.805 [Erysipelotrichaceae bacterium 6_1_45] fig|658657.3.peg.3476 [Erysipelotrichaceae bacterium 21_3] fig|908340.3.peg.3550 [Clostridium sp. HGF2]

fig|552396.3.peg.682 [Erysipelotrichaceae bacterium 5_2_54FAA] fig|457402.3.peg.2428 [Eubacterium sp. 3_1_31]

fig|428127.7.peg.1984 [Eubacterium dolichum DSM 3991] 0.069 0.913 0.340 0.858 0.996 0.000 0.830

0.1

Figure S4. Maximum-likelihood tree for the AgaS proteins. The SEED identifiers for proteins are shown; for their sequences, see the file Sequences S1 in the

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