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Neurophysiological substrates for locomotion-driven eye movements in the mouse
F França de Barros, M. Manuel, P Coulon, H. Bras, D Combes, François Lambert, M. Beraneck
To cite this version:
F França de Barros, M. Manuel, P Coulon, H. Bras, D Combes, et al.. Neurophysiological substrates for locomotion-driven eye movements in the mouse. Institut de Neurosciences de la Timone, Jul 2018, Berlin, Germany. �hal-02413502�
Neurophysiological substrates for locomotion-driven eye movements in the mouse
1 Université Paris Descartes – CNRS, Paris, France
2 Institut de Neurosciences Cognitives et Intégratives d'Aquitaine, Bordeaux, France
3 Institut de Neurosciences de la Timone, Marseille, France
• Clear vision requires the eye to be stable in relation to the visual scene
• Gaze stabilization depends primarily on the sensorimotor transformation of visual and vestibular inputs
• In the Xenopus, there is also a predictive feed- forward signal from the spinal cord central motor pattern generators (CPGs) participating in gaze stabilization during locomotion (Lambert et al., 2012)
Acknowledgements
We thank the Agence Nationale de la Recherche (ANR), the Centre National de la Recherche Scientifique (CNRS) and the Universités Paris Descartes and Bordeaux for their support.
Printed by reprography Université Paris Descartes References
Lambert FM, Combes D, Simmers J, Straka H. 2012. Gaze stabilization by efference copy signaling without sensory feedback during vertebrate locomotion. Curr Biol
Introduction
Find us at
FENS 2018- D019
Conclusion
2. Due to the absence of vestibular and visual inputs during this experiment, the recorded horizontal eye
movements are
coherent with the behavioural output of an efferent copy
1.Neuronal substrates of the efferent copy:
direct neuronal projections from the cervical to the abducens or to the accessory abducens
Our results are the first evidence that spinal-CPG can contribute to gaze stabilization in mammals independently of visual and vestibular sensory inputs.
Investigate the existence of a locomotor-induced gaze stabilizing mechanism in mammals
To do so, this project:
1. Identifies the neuronal substrates of the spino- extraocular pathway: retrograde trans-synaptic tracing of spinal premotor neurons projecting onto abducens motorneurons
2. Investigates its behavioral outputs: videoculography tracking to quantify eye movements during treadmill locomotion of head-fixed, premammilary decerebrated mice
Aims
1. Neuronal substrates of the spino-extraocular pathway
Retrograde injection: lateral rectus or retractor bulbi
muscle I
Rabies virus(RV)
After the injection in the lateral rectus, the distribution of the RV+
premotor interneurons in the cervical:
Bilateral, but mostly ipsilateral, medial and ventral
Mainly located in the cervical’s ventral horn and around the central canal
Both conditions display infected neurons all along the cervical.
Injection on the lateral rectus (nVI marked): wide distribution of marked interneurons in C7- 8. njection on the retractor bulbi (accessory nVI): no neurons marked below C2.
Infection II
55h
III V
45 50 55 60
0 2000 4000 6000
8000 nVI
nVI acc
infection time (hours)
Rostro-Caudal position of infected neurons in the cerv SC (µm)
C3-4
C5-6
C7-8
T1-2 C1-2
IA:: -2
C3-4
C5-6
C7-8
T1-2 C1-2
7n
1760 µm
Acc nVI
7350 µm
0,45 IN/slice
nVI
7500 µm
França de Barros F. 1, Manuel M.1, Coulon P.3, Bras H.3, Combes D.2, Lambert F. 2, Beraneck M. 1
I II
IV
2. Eye movements during treadmill locomotion in decerebrated head-fixed mice
I. Artificial ventilation and anaesthesia: monitoring of vital signs
II. Precollicular and premamillary
decerebration: removal of the corticospinal pathway III. Anaesthesia removal:
ensure reflexive eye movements
IV. Videoculography: record eye movements during spontaneous locomotion
Horizontal eye movements 2-6s after the onset of locomotion
10s long epochs of slow-phase eye movements
Amplitude: 5-10°
Frequency:1-3Hz
III
Degrees(º)
Time (s)
