Do viruses sense their vector ?

(1)

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https://hal.archives-ouvertes.fr/hal-01231286

Submitted on 19 Nov 2015

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Do viruses sense their vector ?

Martin Drucker, Aurélie Bak, Jean Luc Macia, Stéphane Blanc

To cite this version:

Martin Drucker, Aurélie Bak, Jean Luc Macia, Stéphane Blanc. Do viruses sense their vector ?. 15. International Congress on Molecular Plant-Microbe Interactions, International Society for Molecular Plant-Microbe Interactions (IS-MPMI). Saisissez le nom du laboratoire, du service ou du département., Saint-Paul, INT., Jul 2012, Tokyo, Japan. �hal-01231286�

(2)

Martin Drucker

1,2

, Aurélie Bak

1 , Jean-Luc Macia

1 , Stéphane Blanc

1

1 _{Equipe CaGeTE, INRA UMR 385 BGPI, Montpellier, France}

2 _{contact : drucker@supagro.inra.fr}

Noncirculative transmission:

… is often thought to be purely mechanical

Aphid transmission experiments using

protoplasts:

Cauliflower mosaic virus

Circular ds DNA, icosahedral

Turnip mosaic virus

Linear ss RNA, filamentous

0

10

20

30 %

T

ra

n

sm

is

si

on

±

S

D

Effect of the Ca

2+

channel blocker aluminium

Effect of the Ca

2+

channel blocker lanthanum

Control

1 mMLaCl

₃

Control

1 mM AlCl

₃

*

P=0.0001 Mann-Whitney, n=14 P=0.0708 Mann-Whitney, n=10

0

10

20

30 %

T

ra

n

sm

is

si

on

±

S

D

0

20

40 %

T

ra

n

sm

is

si

on

±

S

D

P=0.0009 Mann-Whitney, n=9

0

10

20

30 %

T

ra

n

sm

is

si

on

±

S

D

Virus acquisition from live or dead protoplasts

Live

Dead

Effect of the Ca

2+

channel blocker gadolinium

Control

1 mM GdCl

₃

*

P=0.0571 Mann-Whitney, n=16

0

10

20 %

T

ra

n

sm

is

si

on

±

S

D

0

10

20 %

T

ra

n

sm

is

si

on

±

S

D

Effect of the Ca

2+

channel blocker lanthanum

Control

1 mMLaCl

₃

Effect of the Ca

2+

channel blocker aluminium

Control

1 mM AlCl

₃

*

0

10

20 %

T

ra

n

sm

is

si

on

±

S

D

0

10

20

30 %

T

ra

n

sm

is

si

on

±

S

D

Virus acquisition from live or dead protoplasts

Live

Dead

Effect of the Ca

2+

channel blocker gadolinium

Control

1 mM GdCl

₃

*

* Significant effect (P<0.05)

Discussion:

→ Virus acquisition is not purely mechanical

→ Ca

2+

signaling pathways are involved in acquisition

→ TPC1 channels are not involved in virus-vector recognition

Aphid transmission of both CaMV and TuMV requires live cells

The general Ca

2+

channel blocker lanthanum inhibits transmission

Aluminium does not inhibit transmission

Gadolinium inhibits transmission of TuMV and maybe CaMV → Other Ca

2+

channels are involved

Lin et al. (2005) Biochem BiophysRes Comm 332, 823–830

Aphids?

YES!

Do viruses sense their vector?