Comparative transductions of breast cancer cells by three DNA viruses.

HAL Id: inserm-00143970

https://www.hal.inserm.fr/inserm-00143970

Submitted on 30 Apr 2007

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Comparative transductions of breast cancer cells by

three DNA viruses.

Annick Lucas, Eric Kremer, Silvio Hemmi, José Luis, Françoise Vignon,

Gwendal Lazennec

To cite this version:

Annick Lucas, Eric Kremer, Silvio Hemmi, José Luis, Françoise Vignon, et al.. Comparative trans-ductions of breast cancer cells by three DNA viruses.. Biochemical and Biophysical Research Com-munications, Elsevier, 2003, 309 (4), pp.1011-6. �10.1016/j.bbrc.2003.08.101�. �inserm-00143970�

MDA-MB-231

MDA-MB-435

MDA-MB-436

MCF-7

SKBR-3

T47-D

ZR-75-1

EFM-19

A

B

MDA-MB-231

MDA-MB-435

MDA-MB-436

SKBR-3

EFM-19

MCF-7

T47-D

ZR75-1

% of positive cells % of pos itive cells % of positive cells % of positive cells

Fig. 1

0 20 40 60 80 100 120 0 50 100 400 0 20 40 60 80 100 120 0 50 100 400 0 20 40 60 80 100 120 0 50 100 400 0 20 40 60 80 100 120 0 50 100 400

MOI

MOI

0 20 40 60 80 100 120 0 50 100 400 0 20 40 60 80 100 120 0 50 100 400 0 20 40 60 80 100 120 0 50 100 400 0 20 40 60 80 100 120 0 50 100 400

MDA-MB-231 MDA-MB-435 MDA-MB-436 SKBR-3 EFM-19 MCF-7 T47-D ZR -75-1

α

v

0 0. 4 0. 8 1. 2 1. 6 Arbitra ry Units Arbitra ry Units

CAR

0 0. 2 0. 4 0. 6 0. 8 1. 0 1. 2 MDA-MB-231 MDA-MB-435 MDA-MB-436 SKBR-3 EFM-19 MCF-7 T47-D ZR -75-1

A

B

C

Fig. 2

Re l. flu oresc en ce

α

V

β5

0 2 4 6 8 10 12 14 Rel. fluoresce nce

α

v

0 2 4 6 8 10 12 Rel. fluoresce nce

CAR

0 1 2 3 4 5 6 MDA-MB-231 MDA-MB-435 MDA-MB-436 SKBR-3 EFM-19 MCF-7 T47-D ZR -75-1 CAR α V β5 α V

MDA-MB-436

0

20

40

60

80

100

120

Ad

βgal

CAV

βgal

% of control

α

V Ab

CAR Ab

MCF-7

0

20

40

60

80

100

120

Ad

βgal

CAV

βgal

% of control

α

V Ab

CAR Ab

T47-D

0

20

40

60

80

100

120

Ad

βgal

CAV

βgal

% of control

α

V Ab

CAR Ab

Fig. 3