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High temperatures applied during seed filling of pea (Pisum sativum L.) affect seed growth and nitrogen
partitioning within plant
Valentine Pellissier, Annabelle Larmure, Nathalie G. Munier-Jolain
To cite this version:
Valentine Pellissier, Annabelle Larmure, Nathalie G. Munier-Jolain. High temperatures applied during seed filling of pea (Pisum sativum L.) affect seed growth and nitrogen partitioning within plant. 6th European Conference on Grain Legumes, Integrating legume biology for sustainable agriculture, Nov 2007, Lisbonne, Portugal. pp.12 - 16. �hal-01874573�
D ail y m ean te m p eratu re d u rin g lab e llin g e xp erim e n ts (°C)
2 0 25 30 35
Total exogenous N accumulation (mg.plant-1.day-1) 0 2 4 6 8 1 0 1 2
R ² = 0 ,9 2 ; p < 0 , 0 00 1 Daily mean temperature during treatments (°C)
18.4 23.2 27.9 33.3
Individual seed DM accumulation(mg.seed-1.day-1)
-4 -2 0 2 4 6 8 10 12 14
D a il y m ea n te m pe ra ture d urin g l ab el lin g e xp eri m e nts (°C )
20 25 30 35
Total N remobilised flux (mg.plant-1.day-1)
0 2 4 6 8 10 12 14 16 18
R ² = 0,25; p = 0 ,0330
Remobilized N Exogenous N
High temperatures applied during seed filling of pea (Pisum sativum L.) affect seed growth and
nitrogen partitioning within plant.
- Integrating legume biology for sustainable agriculture - 6thEuropean Conference on Grain Legumes, 12 –16 November 2007, Lisbon, Portugal.
Analysis of temperature effect on elaboration of pea seed yield and quality
Valentine PELLISSIER, Annabelle LARMURE & Nathalie MUNIER-JOLAIN UMR LEG - INRA/ENESAD, Dijon, France - valentine.pellissier@dijon.inra.fr
How are seed weight and seed nitrogen concentration altered by rising temperatures ?
This could originate from a reduction in carbon availability and the modification of nitrogen repartition within plant.
Seed growth response to temperature
Carbon sources Nitrogen sources and partitioning
Conclusions and Perspectives Objectives and methods
Monitoring sources functioning to understand sink responses to temperature
SEED FILLING FSSA
Maturity Pisum sativum L.
cv. Baccara
N2 air High NO3- ӫNon stressing conditions
End of seed abortion
4 temperature treatments:
3-days-labeling experiments (3 to 6 according to T°)
Photosynthesis measurements in growth chamber (independent plants)
+
Day/night
20/15°C
mean : 18.4°C
Day/night
25/20°C
mean : 23.2°C Day/night
30/25°C
mean : 27.9°C
Day/night
35/30°C
mean : 33.3°C
15NO3-= exogenous N 14NO3-= remobilized N
ӫfixation inhibition
N fluxes monitoring: Dry matter monitoring:
Regular plant sampling until maturity Plant compartments : organs (seeds, pods, leaves, stems, roots)
Dry Matter and Nitrogen Accumulation rates
Seed N concentration
Temperature treatment (day/night°C)
15/20°C 20/25°C 25/30°C 30/35°C
% N
3.8 4.0 4.2 4.4 4.6 4.8 5.0
a a
b b
At harvest
0.00 0.05 0.10 0.15 0.20 0.25 0.30 0.35
b Individual seed weight
Dry matter (g)
15/20°C 20/25°C 25/30°C 30/35°C c a a
Maturity dates N sources’ participation
èIncreasing allocation to leaves
with T° èDecreasing allocation to
seeds with T°
with T° with T°
Temperature (°C)
16 18 20 22 24 26 28 30 32 34 36 38
Net photosynthesis (ppmCO2.min-1) 0,0 0,5 1,0 1,5 2,0 2,5 3,0 3,5
R² = 0.73 ; P < 0.0001
Photosynthesis with T°
Daily mean temperature during labelling experiments (°C)
18.4 23.2 27.9 33.3
Individual seed N accumulation rate(µg.seed-1.day-1) -400 -200 0 200 400 600 800
Daily mean temperature during treatments (°C)
18.4 23.2 27.9 33.3
Days from sowing
88 89 90 91 92 93 94 95 96
•Variation of seed growth parameters with temperature rise evolved the same way than main C and N sources variations with temperature èsource alteration by temperature could explain seed growth response: a first step to understand temperature effect on seed filling process in pea
•relevant temperature range = clear variations with temperature of all variables èa modelling prospect can be seen to predict seed filling response to temperature
èLimitation of biomass production at high T°
with T° earlier with T°
èNo accumulations at the highest temperature èdiminution of seed filling duration with T°
Daily mean temperature during labelling experiments18.4 23.2 27.9 33.3
% participation to seed filling
0 10 20 30 40 50 60 70 80 90 100
Remobilised N Exogenous N
èremobilised N is the major source for seed N not affected by T°
