PD-L2 is expressed on activated human T cells and regulates their function.

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HAL Id: inserm-00600726

https://www.hal.inserm.fr/inserm-00600726

Submitted on 10 Jan 2012

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PD-L2 is expressed on activated human T cells and

regulates their function.

Nassima Messal, Nacer-Eddine Serriari, Sonia Pastor, Jacques Nunès, Daniel

Olive

To cite this version:

Nassima Messal, Nacer-Eddine Serriari, Sonia Pastor, Jacques Nunès, Daniel Olive. PD-L2 is ex-pressed on activated human T cells and regulates their function.. Molecular Immunology, Elsevier, 2011, 48 (15-16), pp.2214-9. �10.1016/j.molimm.2011.06.436�. �inserm-00600726�

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B

0 1 2 3 4 5 6 17 5 10 17 IgG1 PD-L2 mAb 0 NS IL-2 n g /m L ** ** * 0 1 2 3 4 5 6 7 8 17 IgG1 17 5 10 PD-L2 mAb 0 NS IFN-γγγγ ** * 0 200 400 600 800 1000 1200 17 5 10 17 IgG1 PD-L2 mAb 0 _[µg/ml] NS IL-10 p g /m L *** * Messal et al Figure 2 IL-2 p g /m L IFN-γγγγ IL-10

A

CD8+ CD4+ _CD4+ _CD8+ _CD4+ _CD8+ * * * * * * * * * * * * 0 200 400 600 800

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C

Messal et al Figure 3 0 1 2 3 4 5 6 0 1 2 3 4 5 6 0 1 2 3 4 5 6 0 1 2 3 4 5 6 0 1 2 3 4 5 6 0 1 2 3 4 5 6

Ig

G

1

0

P

D

-L

2 m

A

b

NS

CFSE

B

A

[µg/ml] 17 5 10 17 0 0.5 1 1.5 2 2.5 D iv is io n i n d e x NS IgG1 PD-L2 mAb [µg/ml]

***

**

17 5 10 17 0

T : aAPC

0 10000 20000 30000

CD4

CD8

Isotype PD-L2 PD1.6.4

***

P ro lif 3 H -T IgG1 PD-1 mAb PD-L2 mAb [ 3H ]-T h y m id in e in c o rp o ra ti o n ( c p m )

CD8

CD4

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PD-L2

βDNA4-PD-L2 Mock

A

N S C D 3 + C D 2 8 Isotypic control PD-L2 mAb (clone 24F.10C12) PD-L2 mAb (clone PD-L2-326.35)

B

Messal et al Supplementary Figure 1 24h 48h

PD-L2

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C

A

Activated T cells

Activated T cells + CsA (100 nM) Isotypic control / activated T cells

PD-L2

Messal et al Supplementary Figure 2 CD3+CD28 stimulation NFAT binding sites 0 2 4 6 8 10 16h 24h PD-L1 R Q * * 0 12 16h 24h PD-L2 * *

B

w/o CsA CsA -- 14 10 8 6 4 2

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



Mouse

Human

 Messal et al Supplementary Figure 3