g lu ta th io n e -d e p e n d e n t fo rm a ld e h y d e d e h y d ro g e n a s e -l ik e

(1)

C A D & C A D -l ik e s

SOR-like

I

II

III

IV

g lu ta th io n e -d e p e n d e n t fo rm a ld e h y d e d e h y d ro g e n a s e -l ik e

other reductase APC8-l.

zinc-bindingdehydrogenasefamily-likeother:paraphyletic CAD-like

Group II

& III

GroupIGroupII&IIIGroupV

Group II

& III Saccharomyces cerevisiaeNP_012693.1 SOR1

Coleochaete scutataChoscu_DN39214_c2_g11_i1 Klebsormidium flaccidumKlefla_DN64741_c1_g1_i14

Marchantia polymorphaMapoly0099s0045.1.p Chara brauniig29945_t1

Klebsormidium flaccidumKlefla_DN62695_c0_g1_i8 Coleochaete scutataChoscu_DN25741_c0_g1_i2 Zygnema circumcarinatumZygcir_DN43052_c0_g1_i1

Mougeotiasp. Mousp_DN14886_c0_g1_i3 Marchantia polymorphaMapoly0096s0075.1.p Mesostigma virideMesvir_DN54269_c0_g1_i2

Coleochaete scutataChoScu_DN36838_c2_g1_i5 Zygnema circumcarinatumZygcir_DN41795_c0_g1_i2 Marchantia polymorphaMapoly0180s0006.1.p Chara globularisChaglo_DN426597_c3_g2_i6

Chara brauniig20450_t1

Mougeotiasp. Mousp_DN12535_c0_g1_i1 Marchantia polymorphaMapoly0033s0043.1.p Klebsormidium flaccidumKlefla_DN62405_c0_g2_i5

Mesostigma virideMesvir_DN28861_c0_g1_i1 Klebsormidium flaccidumKlefla_DN55147_c0_g1_i3

Marchantia polymorphaMapoly0154s0033.1.p Marchantia polymorphaMapoly0021s0075.1.p Spirogyra pratensisSpipra_DN3436_c1_g5_i1

Klebsormidium flaccidumKlefla_DN64186_c0_g1_i2 Marchantia polymorphaMapoly0014s0120.1.p Mougeotiasp. Mousp_DN16720_c0_g1_i6

Spirogyra pratensisSpipra_DN3275_c3_g4_i3 Spirogyra pratensisSpipra_DN3518_c2_g5_i4 Chlorokybus atmophyticusChlatm_DN35353_c1_g1_i7

Spirogyra pratensisSpipra_DN3260_c1_g1_i2 Spirogyra pratensisSpipra_DN3721_c0_g2_i1

Marchantia polymorphaMapoly0003s0299.1.p Chara brauniig38143_t1

Spirogyra pratensisSpipra_DN2955 c2 g1 i4 Marchantia polymorphaMapoly0049s0129.1.p

Mougeotiasp. Mougsp_DN13197_c0_g1_i1 Marchantia polymorphaMapoly0034s0003.1.p

Klebsormidium flaccidumKlefla_DN63577_c0_g2_i1 Marchantia polymorphaMapoly0001s0375.1.p Chara brauniig10943_t1

Chara brauniig10944_t1

Mougeotiasp. Mousp_DN16581_c0_g1_i6 Klebsormidium flaccidumKlefla_DN63416_c0_g1_i11 Chara brauniig80212_t1

Marchantia polymorphaMapoly0005s0036.1.p Chara globularisChaglo_DN380232_c0_g1_i1

Marchantia polymorphaMapoly0073s0049.1.p Marchantia polymorphaMapoly2449s0001.1.p Marchantia polymorphaMapoly0006s0302.1.p

Nitella mirabilisGBST01043702

Klebsormidium flaccidumKlefla_DN64757_c1_g2_i16 Zygnema circumcarinatumZygcir_DN44296_c0_g1_i8

Ostreococcus lucimarinus13349 Mesostigma virideGBSK01010231

Chlorokybus atmophyticusChlatm_DN36474_c3_g4_i8 Chlamydomonas reinhardtiiCre03.g207800.t1.2

Coleochaete orbicularisGBSL01017510 Arabidopsis thalianaAT3G19450.1 CAD4 Arabidopsis thalianaAT4G34230.1 CAD5 Picea abiesMA_104401g0010 Selaginella moellendorffii444096

Arabidopsis thalianaAT4G37970.1 CAD6 Arabidopsis thalianaAT4G37980.1 CAD7 Arabidopsis thalianaAT4G37990.1 CAD8 Arabidopsis thalianaAT4G39330.1 CAD9 Arabidopsis thalianaAT2G21730.1 CAD2 Arabidopsis thalianaAT2G21890.1 CAD3 Selaginella moellendorffii230239 Picea abiesMA_423264g0010 Picea abiesMA_18380g0010

Marchantia polymorphaMapoly0073s0084.1.p Marchantia polymorphaMapoly0050s0130.1.p

Marchantia polymorphaMapoly0032s0157.1.p Marchantia polymorphaMapoly0032s0159.1.p Marchantia polymorphaMapoly0032s0158.1.p Marchantia polymorphaMapoly0032s0162.1.p Marchantia polymorphaMapoly0517s0001.1.p Physcomitrella patensPp3c1_39700V3.1.p Klebsormidium nitenskfl00162_ 0110

Klebsormidium flaccidumKlefla_DN62893_c0_g3_i4 Arabidopsis thalianaAT1G72680.1 CAD1

Selaginella moellendorffii149351

Marchantia polymorphaMapoly0004s0065.1.p Marchantia polymorphaMapoly0004s0064.1.p Marchantia polymorphaMapoly0004s0063.1.p Physcomitrella patensPp3c2_10530V3.1.p Mougeotiasp. Mousp_DN13563_c0_g1_i3 Spirogyra pratensisSpipra_DN3516_c1_g4_i3 Spirogyra pratensisSpipra_DN2816_c0_g1_i2 Spirogyra pratensisGBSM01011485 55

58 99 73 72 100 100

59 77 100 98

100

54

100

5057

100 95 59 100

57

51

59

60 100

100

98 53

100 97

100

77 77 100

100 100 75

97

94

100 95

75

65

100 99 100

100 72

100 100 94

100 100

86

81 100 100 53

100

69 100 68 94

81 90 1 100

Figure S1. Phylogeny of CAD-related sequences.

Sequences were identified based on a BLASTp/tBLASTn search with CAD sequences from Arabidopsis thaliana as query against a sequence database of genomic and transcriptomic data from bryophytes and streptophyte algae.

Additional sequences that were identified in de Vries et al. (2017) were also included.

Sequences from transcriptomic data from this study and de Vries et al. (2018) were filtered and only sequences with a TPM≥1 in at least one condition were included. The phylogeny is based on a full-length sequence data, which was aligned using MAFFT using the option G-INS-I.

g lu ta th io n e -d e p e n d e n t fo rm a ld e h y d e d e h y d ro g e n a s e -l ik e

C A D & C A D -l ik e s

g lu ta th io n e -d e p e n d e n t fo rm a ld e h y d e d e h y d ro g e n a s e -l ik e

94

65

Figure S1. Phylogeny of CAD-related sequences.

Sequences were identified based on a BLASTp/tBLASTn search with CAD sequences from Arabidopsis thaliana as query against a sequence database of genomic and transcriptomic data from bryophytes and streptophyte algae.

Additional sequences that were identified in de Vries et al. (2017) were also included.

Sequences from transcriptomic data from this study and de Vries et al. (2018) were filtered and only sequences with a TPM≥1 in at least one condition were included. The phylogeny is based on a full-length sequence data, which was aligned using MAFFT using the option G-INS-I.

Annotation for CAD and CAD-like

sequences is based on de Vries et al. 2017

and annotation of the other sequences is

based on a BLASTp search using the

sequences as query and the genome of the

A. thaliana TAIR10 release as the

database. The e-value cutoff for all

BLASTp searches was 10

. SOR-like

sequences were used as outgroup.