HAL Id: hal-02805274
https://hal.inrae.fr/hal-02805274
Submitted on 6 Jun 2020
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Project: ANAEE-S
Laurent Saint-André, Jean Clobert, Andre Chanzy
To cite this version:
Laurent Saint-André, Jean Clobert, Andre Chanzy. Project: ANAEE-S:
Analysis and Experimentation on Ecosystems – Service. ANAEE-S, 2013, pp.16 slides. �hal- 02805274�
Titre du projet en français : Infrastructure nationale “Analyse et d’expérimentation sur les écosystèmes – Service”
Project title in English Analysis and Experimentation on Ecosystems – Service
Project: ANAEE-S
Coordination
Jean CLOBERT , CNRS, Station d’Ecologie Expérimentale de Moulis
Cocoordination
André CHANZY, INRA, EMMAH, UMR 1114
Requested funding:
Phase 1 (2012-2015) 9,753,607 € Phase 2 (2015-2020) 4,427,734€
Scientific area
Ecology, agronomy, ecosystem biology, evolutionary biology, complex system
analysis
Participants :
Project: ANAEE-S
+ UREP [EFPA], EEF [EFPA], EGC [EA] (via BEF - Instrumentation) + SOERE F-ORE-T (via BEF – sites ateliers)
EFPA
Project: ANAEE-S
Social context : how to best produce goods while at the same time preserving ecological services
Scientific question : how understand and predict biodiversity and ecosystems dynamics under global changes ?
(abiotic and biotic processes, strong temporal and spatial heterogeneity, coupled ecological and fast evolutionary dynamics, and a huge diversity from genes to communities)
Scientific needs to solve the question : new approaches that bridge the
traditional gap between life and environmental sciences are necessary.
Objectives of the project: ANAEE-S will bring together, an integrated network of
the major experimental, analytical and modeling platforms dedicated to the
biological study of continental ecosystems (aquatic and terrestrial).
Project: ANAEE-S
Overall project structure
Ecotrons de Montpellier et
Ile de France
3SEMI-NATURAL experimental platforms CEREEP-PLANAQUA (CNRS), SEEM-Métatron (CNRS) and the U3E-Aquatic Platform (INRA).
SOERE F-ORE-T (14), ACBB(4), PRO (3), GLAPCE (1)
BEF, UREP, EEF [EFPA]
GENOSOL, PESSAC [EA] ECO-INFORMATIQUE
INRA (Capsis, Record,
Sol Virtuel) + CMTB
(CNRS)
Project: ANAEE-S
Project principles :
Strengthen the attractiveness of the involved structures by providing funds -to maintain each node (equipment, infrastructure)
-to buy and develop innovative instruments in each node
-to build interactive tools interconnecting data and models provided by the nodes
Share services within and between nodes
- to enhance our understanding of the functioning of terrestrial and aquatic ecosystems by combining studies on biotic and abiotic
factors
- to further develop researches at the interface between ecosystems (region scale, multidisciplinary approach, multi-ecosystems)
Propose these services to the international community by
- facilitating access to the metadata provided by the nodes
- defining rules of access and cooperation
Node 1 : Experimental in vitro platforms – TGIR Ecotrons CNRS
Ex-situ Controlled manipulation of terrestrial and
aquatic organisms, communities and ecosystems
ANAEE-S will provide new equipments:
Small climate controlled chambers coupled with gas/isotopes analysers + Wetland
lysimeters in Montpellier;
Microcosms dedicated to the manipulation
and detailed monitoring of freshwater or
marine algal and zooplankton communities
in Ile de France;
Node 2: Semi-natural experimental facilities
Semi-natural controlled manipulation of
terrestrial and aquatic organisms, communities and ecosystems
ANAEE-S will :
Provide upgraded facilities, equipments and services + automation of data acquisition (monitoring, remote
transmission), management, archiving and access for U3E Contribute to the development of aquatic macrocosms (artificial lakes) adapted to the long-term manipulation and monitoring of complex aquatic ecosystems in CEREEP
Contribute to the renovation of the METATRON (including a
better control of the water table) + funding of a RMI device in
SEEM
Node 3: In-Natura experimental facilities
In-Natura long-term observation and experimentation systems
ANAEE-S will :
Provide maintenance facilities for F-ORE-T and will contribute to the renovation of two buildings for SAJF Contribute to the development of off shore aquatic mesocosm platform for GLAPCE
Develop facilities on SOERE PRO experimental platform (croplands) to host further experimental devices (electricity power, bungalow, cooling and freezing capacity) and provide continuous acquisition of GHG on site
Completion of the canopy Operating Permanent Access
System COPAS and installation of a sensor grid in Les
Nouragues in French Guyana
Node 3: In-Natura experimental facilities Projet UMR CARRTEL SOERE Lacs Alpins
Dia: 36m (plateforme 8m)
Sacs: h=12m, dia = 2,5
Mésocosmes
Node 4 : Shared instruments and analytical platforms dedicated to environmental microbiology
Innovative devices and platforms for in situ analysis and for the microbiological compartment
ANAEE-S will :
Provide a MobileLAB (i) to characterize sites (soil, vegetation, water)- tests in situ in node 2 and 3 and application in a large range of ecosystems, (ii) to perform targeted experiments (hotspots and hot moment), and (iii) to host innovative techniques
Develop innovative and shared instruments (VOC signature of thje microbial communities measured by Proton Transfer Reaction-Mass spectroscopy (PTR-MS) method; continuous measurement of stable isotope of CO2 (δ13C) from soil efflux using The Cavity Ring-Down Spectroscopy (CRDS)
Develop Microbiology analytical platforms for environmental matrix (soil, water, sediment), including biodiversity characterization (genetic and genomic tools) and the biochemical characterization
(biochemical tools)
Node 4: Shared instrument facilities - MobileLab
OBJECTIVES: bring the lab on the field:
- characterize terrestrial ecosystems (soil, vegetation) - develop innovative in-situ measurements
- being on the right place at the right moment (hotspots, capture extreme events)
M-POETE, Mobile Plateform for the Observation and the Experimentation in Terrestrial Ecosystems
Projet UR BEF
York Environmental Sustainability Institute
Muppet
mobileLab U.S. space agency NASA
SBS Mobile Freezing Labs National Air and Radiation Environmental Laboratory
Network of measure devices (4) able to measure continuously (1Hz)13C, CO2, H2O and CH4 concentration using
gradient chambers technique, or others (atmosphere), at 8 sample points to determine soil efflux, canopy gas
exchange, ect to determine C transfer plant-soil systems.
Example
unit total
CRDS analyser, Picarro 55000 55000 220000
Closed trailer 1500
Data acquisition computer 1500
2 Pompes 1500
MassFlowControllers 3000
CR3000, 8 electronic valves, SDM 3000
tubing +conncections 1000
Isolation+ventilation 500
12000 48000
268000 12m technicien+ 24m ingeneer
Node 4: Shared instrument facilities - CRDS Projet UMR UREP
Node 4: Shared instrument facilities – PTR-MS
Résolution permettant de différentier les différents COVs
Objectifs: « Quantifier les flux d’une panoplie de COVs émis par les sols et les PROs comme
indicateur de leur diversité biologique»
1 – Mise en œuvre du PTR-MS et test en labo (précision)
2 – Mise au point des mesures en chambre et Eddy Covariance et tests au champ
3 – Test du transfert des COVs dans les sols au labo
4 – Calibration de la méthode par comparaison à des profils DNA Pilotage :
Benjamin Loubet (INRA, EGC) Pierre Alain Maron (INRA, MSE)
+ Post-doc 2.5 ans, ingénieurs, techniciens
Projet UMR EGC et UMR MSE
Node 4: Environmental microbiology platform
Genetic and genomic tools (GENOSOL – MSE)
National Genetics Resources Centre (CRG) for samples from ANAEE-S
responsible for the storage and conservation of genetic resources (DNA) and making them available to the scientific community (strong links with the mobileLab)
Technical platform for the development and technological surveillance of methods for extracting nucleic acids and tools for characterizing microbial genetic
resources.
Standardization of procedures and molecular tools, with a view to normalization (AFNOR and ISO) strong partnership with Genoscope
Environmental Information System (SIE) centred on the development of data base
Management of CRG and sample traceability and secondly, data storage and analysis concerning genetic characterization (taxonomic, functional) – strong links with node 5.
Biochemical tools (Biochem-Env– PESSAC)
Acquisition and characterization of biochemical properties of different solid matrix (soil and sediments)
collected in sites of node 1 to 3.Development of referentials and tools of interpretation.
Availability of these resources to the scientific community and the policy makers.
Produce a reliable reference system based on the biochemical characterization (functional feature) of natural media that will permit scientific interpretation of analyses.
