Charged surface area of maurocalcine determines its interaction with the skeletal ryanodine receptor.

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Submitted on 29 Apr 2009

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Charged surface area of maurocalcine determines its

interaction with the skeletal ryanodine receptor.

Balázs Lukács, Mónika Sztretye, János Almássy, Sándor Sárközi, Beatrix

Dienes, Kamel Mabrouk, Cecilia Simut, László Szabó, Péter Szentesi, Michel

de Waard, et al.

To cite this version:

Balázs Lukács, Mónika Sztretye, János Almássy, Sándor Sárközi, Beatrix Dienes, et al.. Charged surface area of maurocalcine determines its interaction with the skeletal ryanodine receptor.. Biophys-ical Journal, BiophysBiophys-ical Society, 2008, 95 (7), pp.3497-509. �10.1529/biophysj.107.120840�. �inserm-00376509�

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Fig 1. Control 200 nM MCa 200 nM Mca + 1 µM Ryanodine

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MCa + Rya Rya 10 pA 1 s 50 µM Ca2+ _{240 nM Ca}2+

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Fig 2 10 pA 2 s 200 nM [Ala8] MCa 200 nM MCa 20 ms

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200 nM [Ala22] MCa 200 nM [Ala20] MCa 200 nM [Ala23] MCa 200 nM [Ala24] MCa

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200 nM [Ala19] MCa

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Fig 3 mutations wt 8 19 20 22 23 24 L L S S r a ti o ( % ) 0 20 40 60 80 100 50nM 200nM 500nM mutations wt 8 19 20 22 23 24 L L S S l e n g th ( m s ) 0 5000 10000 15000 50nM 200nM 500nM

A

B

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Fig 4 2 F0 200 ms 20µ m 200 ms 20µm A B C

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A

B

a

b

500 ms 5 µm 1000 ms 20 µm F₀ 0.2 F/F₀ 0.2 F/F₀ Fig 5

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A B C D E Fig. 6

4 F

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Mutations

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22 WT

Amplitude

FWHM

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Fig 7