Crystal structure of a novel type of odorant binding protein from Anopheles gambiae, belonging to the C+ class
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(2) Biochemical Journal Immediate Publication. Published on 12 May 2011 as manuscript BJ20110522. us cr ipt. ! " # $ # % & . . . . ! " # $"% & ' $($( ) *. ce. pt. ed. M. an. + , # ' # - " ' # - " !% . /(. -0'% , 1 2 % )13$$%. %(%4( * 561 2 7#2% "6%# 1! # 3 8 - 9 ': 1 # ', , 2 2 , , 2 , , % . Ac. THIS IS NOT THE VERSION OF RECORD - see doi:10.1042/BJ20110522. (. . Licenced copy. Copying is not permitted, except with prior permission and as allowed by law. © 2011 The Authors Journal compilation © 2011 Portland Press Limited. .
(3) ce. pt. ed. M. an. us cr ipt. % ' ( ) % *+ ( , (- . ) % , % % ( - /0 1, ( ! , /0 % 23 , 4 5 % ( 23 1 ) % ( , 1 ) ) 1 , ) % % ( % ( % 23 % , ( , % 23 , ' % , 6 % ( % 1% % , . ( 1--1, 2 , *8-1 2 , * ;8-1 ; 2 , * 5! 1 5 , !' '* 818# ' , *! -1! ' 2 - *1 " %. Ac. THIS IS NOT THE VERSION OF RECORD - see doi:10.1042/BJ20110522. Biochemical Journal Immediate Publication. Published on 12 May 2011 as manuscript BJ20110522. . Licenced copy. Copying is not permitted, except with prior permission and as allowed by law. © 2011 The Authors Journal compilation © 2011 Portland Press Limited. (.
(4) Biochemical Journal Immediate Publication. Published on 12 May 2011 as manuscript BJ20110522. ce. pt. ed. M. an. us cr ipt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c. THIS IS NOT THE VERSION OF RECORD - see doi:10.1042/BJ20110522. . . Licenced copy. Copying is not permitted, except with prior permission and as allowed by law. © 2011 The Authors Journal compilation © 2011 Portland Press Limited. $.
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(6) ce. pt. ed. M. an. us cr ipt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c. THIS IS NOT THE VERSION OF RECORD - see doi:10.1042/BJ20110522. Biochemical Journal Immediate Publication. Published on 12 May 2011 as manuscript BJ20110522. . Licenced copy. Copying is not permitted, except with prior permission and as allowed by law. © 2011 The Authors Journal compilation © 2011 Portland Press Limited. /.
(7) ce. pt. ed. M. an. us cr ipt. ' ), %> -0! " <.= # , " '% 02 # # $ R(4%/I # # % -0! B , ) 2 : " ; #%9 , 9 #A(% : ' 8- # , : ',% 23, + & 2 6 8- # +, 8-.4 ' 2 & % E : " 2 : 2 : : : ' : # 2 ? %$@%F $+ #8-.4: , , # & # ' , 2 # , " ,, ? % $ " !@% " ' , , 2 : 8-.4 & 1EE$E. E/: 8-.4E4:& E?>2 (@%E )( # 2 8- , ## , , : ) ?E E @ 2 " 8-.4 ? % $@% F " : , 6 " # 6, 2 8- " ? % $ +5@% > : " # # # # # # 2 : " 6 " ? %$+5@% 23 % > # #8-.4 " # # 8-%> "' #8- 2 " 8-.42 : : " 2 " ,, # B 2' # R ? % ( $+5 !( " !(@% ? % !(@ A ( R ) R . R ,% > , # " ' # 2' 6 /6$.$ 6.( ? % !( 2 @ : # # # : "' : # 2' 6 /64$ ? %!( @%8# 2 )' , # & 4$# ' 2 :E$. +# # 6 8-<$/ ( =%> : # "' # 2' ' , 2 #" ?- >'@ ) , ?& N @ - ;' 6, >?>2 $@% (? %!(@ A.R)R .R ,%> , # " # 2' 6 6 ? %!( +2 @ 2' 6 /64$ : , # # , ? % !( @% > 6 # : 2 : : " % > , # " ) ./6/(? %!( + @ $6( ? % !( + , B @ $/6.$ ? % !( + ' : @% > : # 2 # 2' ' , ?>2 $@ 2 #: " ? %!( + @%> 2 # "' , : 8- & # # , , : "' & %> 2 6 6 ($6(/ $4. ./ , " ' # # )E.? & 44@ )E/?>' @% > " 2 ,' ' #8-.4: , # , ):.6' )'6.S6 , ,'6H 2 H ?T8@ 2 #8-.4 <$ =% F B H ' : 2 B' # : " % F , # ' , 5 %> " T8#: "' B : # # " ? %!$>2 $ " !(@%. Ac. THIS IS NOT THE VERSION OF RECORD - see doi:10.1042/BJ20110522. Biochemical Journal Immediate Publication. Published on 12 May 2011 as manuscript BJ20110522. . Licenced copy. Copying is not permitted, except with prior permission and as allowed by law. © 2011 The Authors Journal compilation © 2011 Portland Press Limited. .
(8) ce. pt. ed. M. an. us cr ipt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c. THIS IS NOT THE VERSION OF RECORD - see doi:10.1042/BJ20110522. Biochemical Journal Immediate Publication. Published on 12 May 2011 as manuscript BJ20110522. . Licenced copy. Copying is not permitted, except with prior permission and as allowed by law. © 2011 The Authors Journal compilation © 2011 Portland Press Limited. 4.
(9) ce. pt. ed. M. an. us cr ipt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c. THIS IS NOT THE VERSION OF RECORD - see doi:10.1042/BJ20110522. Biochemical Journal Immediate Publication. Published on 12 May 2011 as manuscript BJ20110522. . Licenced copy. Copying is not permitted, except with prior permission and as allowed by law. © 2011 The Authors Journal compilation © 2011 Portland Press Limited. .
(10) ce. pt. ed. M. an. us cr ipt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c. THIS IS NOT THE VERSION OF RECORD - see doi:10.1042/BJ20110522. Biochemical Journal Immediate Publication. Published on 12 May 2011 as manuscript BJ20110522. . Licenced copy. Copying is not permitted, except with prior permission and as allowed by law. © 2011 The Authors Journal compilation © 2011 Portland Press Limited. .
(11) ce. pt. ed. M. an. us cr ipt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c. THIS IS NOT THE VERSION OF RECORD - see doi:10.1042/BJ20110522. Biochemical Journal Immediate Publication. Published on 12 May 2011 as manuscript BJ20110522. . Licenced copy. Copying is not permitted, except with prior permission and as allowed by law. © 2011 The Authors Journal compilation © 2011 Portland Press Limited. .
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