Processing of human cathepsin D is independent of its catalytic function and auto-activation: involvement of cathepsins L and B.

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HAL Id: inserm-00153742

https://www.hal.inserm.fr/inserm-00153742

Submitted on 11 Jun 2007

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Processing of human cathepsin D is independent of its

catalytic function and auto-activation: involvement of

cathepsins L and B.

Valérie Laurent-Matha, Danielle Derocq, Christine Prébois, Nobuhiko

Katunuma, Emmanuelle Liaudet-Coopman

To cite this version:

Valérie Laurent-Matha, Danielle Derocq, Christine Prébois, Nobuhiko Katunuma, Emmanuelle Liaudet-Coopman. Processing of human cathepsin D is independent of its catalytic function and auto-activation: involvement of cathepsins L and B.. Journal of Biochemistry, Oxford University Press (OUP), 2006, 139 (3), pp.363-71. �10.1093/jb/mvj037�. �inserm-00153742�

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a

_{Figure 2}

51-47K Processing intermediates E-64 CD55-/-cath-D CD55-/-D231N_cath-D 34K

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+

-

+

48K 52K 33K 47K 51K 52-48K Processing intermediates

b

E-64 (mM) 0 0.1 0.5 1 5 10 52K 48K 34K 52-48K Processing intermediates

c

+ E-64 52-48K Processing intermediates 52K 48K Isoelectric pH 5.9 - E-64 5.2 5.9 5.2 CD55-/-cath-D 34K 52K 48K 0 CA-074Met E-64 CD55-/-D231N_cath-D 0 CA-074Met E-64 51K 47K 33K

d

CD55-/-cath-D CD55-/-D231N_cath-D CA-074Met (µM) 0 0 0 50 50 50 0 0 0 50 50 50 CLICK-148 (µM) 0 5 20 0 5 20 0 5 20 0 5 20 34K 52K 48K 33K 51K 47K