HAL Id: hal-02081482
https://hal-normandie-univ.archives-ouvertes.fr/hal-02081482
Submitted on 27 Mar 2019
HAL is a multi-disciplinary open access
archive for the deposit and dissemination of sci-entific research documents, whether they are pub-lished or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers.
L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.
Small molecules interfere with the tip-polarized growth
of Arabidopsis and tomato pollen tubes
Ferdousse Laggoun, Flavien Dardelle, Denis Falconet, Azeddine Driouich,
Patrice Lerouge, Arnaud Lehner, Jean-Claude Mollet
To cite this version:
Ferdousse Laggoun, Flavien Dardelle, Denis Falconet, Azeddine Driouich, Patrice Lerouge, et al.. Small molecules interfere with the tip-polarized growth of Arabidopsis and tomato pollen tubes. 17ième Journées de l’Ecole Doctorale NBISE, Apr 2014, Le Havre, France. �hal-02081482�
To date, the different mechanisms that occur during the tip-polarized growth,
guidance and adhesion of pollen tubes through the female transmission tract
tissue are not well known.
III. Aim of this work
To investigate these different mechanisms in vitro.
II. Problematic
Using a chemical
screen approach
IV. Previous work:
Using a chemical screen on 300
small
bioactive
molecules
originating from the CERMN
(Centre d’étude et de Recherche
du Médicament de Normandie)
8 compounds were selected
3 for their abilities to perturb Arabidopsis thaliana pollen tube growth and
shape.
5 for their inhibiting effects on the adhesion of pollen tubes in vitro on a matrix
composed of pectin.
V. Materials and Methods
Flowers of A. thaliana and tomato (S. lycopersicum )
Extraction of pollen grains and culture for 6 h in a germination
medium containing the molecules
1/ Evaluation of
the dose
dependent
effect
2/ Select the
concentrations
to obtain a
phenotype
VII. Conclusion and perspectives :
The molecules have a dose-dependent effect on Arabidopsis and tomato
pollen tubes.
Use the selected concentrations to investigate their effects on the cell wall
deposition by immuno-cytochemistry and on the cytoskeleton organization.
VI. Preliminary results
Figure 1: The dose-dependent effects of the molecules on (A) the pollen tube length and (B) phenotypes of
Arabidopsis pollen tubes treated with the molecules. Red arrows : increasing concentration, blue arrow :
dose-dependent effect on pollen tube length. Galv = Galvestine. Scale bar = 20µm.
Figure 2: Ratio of the phenotypes and morphology of A. thaliana pollen tubes. Galv =
Galvestine. PT : Pollen tube. Scale bar = 40 µm.
Figure 3: Ratio of phenotypes and morphology of S. lycopersicum (tomato) pollen tubes. Galv =
Galvestine. PT : Pollen tube. Scale bar = 40 µm.
I. Introduction
Sexual reproduction in land plants is of
economic importance because it allows
the production of seeds. The process
starts when the pollen grain lands on the
stigma, rehydrates and forms a pollen
tube
that
grows
through
the
transmission tact tissue and deliver the
two gamete cells to the ovule.
.
Stigma
Pollen grain
Pollen tube
Transmission
tissus
Ovules
0
200
400
600
800
1000
1200
Control
DMSO 0,3%
Galv
m42
m146
0
100
200
300
400
500
600
700
800
900
Control DMSO
0,3%
Galv
m42
m146
P
oll
e
n
tub
e
le
n
gth
(µ
M)
Molecules
0
100
200
300
400
500
600
700
800
900
Control DMSO 0,3%Galv
m42
m146
P
ol
le
n
tub
e
le
ng
th
(µ
m
)
Molecules
0,5µM
1µM
5µM
10µM
20µM
30µM
P
oll
e
n
tub
e
le
n
gth
(µ
M)
Molecules
0
200
400
600
800
1000
1200
Control DMSO
0,3%
Galv
m42
m146
P
ol
le
n
tub
e
le
ng
th
(µ
m
)
Molecules
0,5µM
1µM
5µM
10µM
20µM
30µM
Arabidopsis
Tomato
30µM
0,5µM
1µM
5µM
10µM
20µM
30µM
0,5µM
1µM
5µM
10µM 20µM
30µM
0,5µM
1µM
5µM
10µM
20µM
A
B
Galv
m42
m146
57%
10%
33%
Control DMSO 0,3%
Normal PT
PT with a
phenotype
Burst PT
33%
57%
10%
39%
12%
49%
11%
77%
12%
60%
40%
m42 10 µM
Galv 10 µM
Control
m146 20 µM
13%
69%
18%
19%
3%
78%
100%
21%
6%
73%
57%
10%
33%
Control DMSO 0,3%
Normal PT
PT with a
phenotype
Burst PT
Control
m42 5 µM
Galv 10 µM
m146 10 µM
Small molecules interfere with the tip-polarized growth
of Arabidopsis and tomato pollen tubes
Laggoun Ferdousse
1
, Dardelle Flavien
1,2
, Falconet Denis
3
, Driouich Azeddine
1,4
, Lerouge Patrice
1
, Lehner
Arnaud
1
and Mollet Jean-Claude
1
.
1
Laboratoire Glycobiologie et Matrice Extracellulaire Végétale, EA4358, IRIB, Normandy University, University of Rouen, 76821 Mont Saint-Aignan, France.
2
Present address : Fitoplanton Marino, S.L. 11500 El Puerto de Santa María, Cádiz, Spain.
3
Physiologie Cellulaire & Végétale (PCV), UMR 5168 - CEA - CNRS - INRA - Université Joseph Fourier, 38054 Grenoble, cedex 9, France.
4
Plate-forme de Recherche en imagerie de Haute Normandie (PRIMACEN), IRIB, Normandy University, University of Rouen, 76821 Mont Saint-Aignan, France.
EdN
BISE
Ecole doctorale Normande
Biologie Intégrative, Santé, Environnement
