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Determination of Rapid-Equilibrium Kinetic Parameters of Ordered and Random Enzyme-Catalyzed Reaction A + B = P + Q

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Academic year: 2021

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TABLE 1: Kinetic Parameters for Ordered A + B ) Ordered P + Q Calculated from Velocities at {100,100,0,0}, {1,100,0,0}, {100,1,0,0}, {0,0,100,100}, {0,0,1,100}, and {0,0,100,1} V fexp V rexp K IA K IQ K P K B K′ no errors 1.00 0.50 5.00 10.00 15.00 20.00 3
Table of Velocities Calculated for the Three Mechanisms.
TABLE 6: Velocities Calculated for Three Different Mechanisms at Eight Quadruplets of Substrate Concentrations ([A],[B], [P],[Q]) ord A + B )ord P+Q ord A + B )rand P+Q rand A + B )rand P+Q (100,100,0,0} 0.8264 0.8265 0.8043 {1,100,0,0} 0.4545 0.4545 0.180

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