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Selection for intrabody solubility in mammalian cells using GFP fusions.: Soluble intrabody selection in mammalian cells

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HAL Id: inserm-00633286

https://www.hal.inserm.fr/inserm-00633286

Submitted on 13 Oct 2012

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Selection for intrabody solubility in mammalian cells

using GFP fusions.

Laurence Guglielmi, Vincent Denis, Nadia Vezzio-Vié, Nicole Bec, Piona

Dariavach, Christian Larroque, Pierre Martineau

To cite this version:

Laurence Guglielmi, Vincent Denis, Nadia Vezzio-Vié, Nicole Bec, Piona Dariavach, et al.. Selection for intrabody solubility in mammalian cells using GFP fusions.: Soluble intrabody selection in mammalian cells. Protein Engineering, Design and Selection, Oxford University Press (OUP), 2011, 24 (12), pp.873-81. �10.1093/protein/gzr049�. �inserm-00633286�

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B

A

MW 2F12 2G4 2F12 2G4Soluble Insoluble 35 25 15 35 55 70 100 130 25

D

2F12 2G4 2F12 2G4 2F12 2G4 Soluble Insoluble 1x 10x 25 35 GAPDH 0.16 0.96 0.06 0.01 1.01 0.01 scFv GAPDH

C

HeLa 13R4 2F12 2G4 35

D

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