Recombinant human interleukin-12 is the second example of a C-mannosylated protein

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Figure

Fig. 1. Structure of C 2 -α-mannosyltryptophan [(C 2 -Man-)Trp].

Fig. 2. Characterization of the modified C-terminal peptide from rHuIL-12. (A) Reduced and carboxamidomethylated rHuIL-12 was digested with trypsin, and the two C-terminal peptides (a and b) were isolated by LC-ESIMS (upper panel) and characterized by nano

Table I. Chemical shifts and J-couplings of the C-glycosylated amino acid residue, obtained at 300 K

$Fig. 4. In vitro C-mannosylation of synthetic IL-12 peptide. (A) The radiolabeled peptide was digested with trypsin and fractionated by C8 reversed phase HPLC$

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