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Publisher’s version / Version de l'éditeur:

Future Virology, 3, March 2, pp. 99-103, 2008

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Poxvirus-based vaccine platforms: getting at those hard-to-reach

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Nino-Fong, Rodolfo; Johnston, James B.

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part of

Poxvirus-based vaccine platforms: getting at

those hard-to-reach places

Rodolfo Nino-Fong & James B Johnston

Author for correspondence

Institute for Nutrisciences and Health, National Research Council Canada, 550 University Avenue, Charlottetown, PE, C1A 4P3, Canada Tel.: +1 902 566 8007; Fax: +1 902 367 7539; james.johnston@nrc.ca

‘The prominence of poxviruses as

candidate vaccine vectors

completes a circle begun in the

18th Century when Jenner first

tested the theory of vaccination

using Cowpox virus to inoculate

against smallpox infection.’

The poxviruses are a large family of dsDNA viruses that includes some of the most notorious and extensively researched pathogens, most notably Variola virus, the causative agent of smallpox, and Vaccinia virus (VV), the prototypical poxvirus. Poxviruses are also notable among viruses for their large virion and genome sizes, the ability to repli-cate autonomous of host cell nuclear machinery, and the apparent absence of a virus-specific surface moiety for cell entry [1]. Historically the subjects of

extensive study because of their pathogenesis in humans and domestic animals, the eradication of smallpox as a human health concern in the 1980s greatly curtailed basic research involving poxvi-ruses. In recent years, new avenues of biotherapeu-tic research, collectively known as virotherapeubiotherapeu-tics, have arisen to counter this trend, with the creation of numerous recombinant poxviruses for use as vaccine platforms, vectors for gene delivery and oncolytic agents in the treatment of cancer [2–4].

The prominence of poxviruses as candidate vac-cine vectors completes a circle begun in the 18th Century when Jenner first tested the theory of vac-cination using Cowpox virus to inoculate against smallpox infection. Today, vaccination remains a critical intervention for preventing and limiting the spread of disease in the general population, as exemplified by the current campaign to vaccinate adolescent girls against human papilloma virus [5].

Despite many successes, effective vaccines for numerous prevalent conditions are lacking even as new infectious agents emerge and necessitate improved vaccination tools.

The capacity to genetically engineer poxviruses to alter pathogenicity and express heterologous genes encoding foreign antigens was being demon-strated even as smallpox was being eradicated. This

ability initiated the era of VV as a eukaryotic expression vector with broad potential as a vector-based vaccine candidate. The properties of poxvi-ruses that make them amenable for use as immu-nizing agents are well documented [6,7]. From a

practical perspective, poxvirus vectors are stable in lyophilized form and are comparatively cheap and easy to manufacture. They have the potential to be safely administered by multiple pathways and elicit both mucosal and systemic responses. Compared with smaller viral vectors, the poxvirus genome can accommodate large foreign DNA inserts without disrupting viral stability, supporting multivalent delivery systems targeting several antigens from a single pathogen or antigens from several different pathogens. The lack of a restrictive surface receptor for entry also increases the range of hosts and cell types against which poxviruses may be applied. The principal flaw inherent in poxviral vectors is the issue of safety in the general population. The same adverse complications associated with the Dryvax vaccine used in the smallpox campaign also apply to vaccine vectors based on live VV recombinants. Attempts to address this concern include attenuated VV strains, such as modified vaccinia Ankara (MVA) and NYVAC, with improved safety potential due to the loss of key pathogenic genes [8,9]. Avipoxvirus vectors based on

Canarypox virus (CPV) and Fowlpox virus (FPV), such as ALVAC and TROVAC, are also extremely attractive from a biosafety standpoint [10,11]. Like

other poxviruses, Avipoxviruses can gain entry to a

wide variety of cell types but infection is aborted in nonavian cells. In addition, Avipoxvirus vectors do

not cross-react with VV and elicit weaker vector-directed immune responses in mammals, support-ing a multiple boostsupport-ing regimen of treatment. Similarly, vectors derived from other poxvirus gen-era may also offer safer alternatives to VV [12,13].

‘The principal flaw inherent in poxviral vectors is the issue of safety in the

general population.’

Once their potential was recognized, numer-ous poxvirus vectors were engineered against diverse viral, bacterial and parasitic agents [14–16],

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2

EDITORIAL

– Nino-Fong & Johnston

Future Virol. (2008) 3(2) future science groupfuture science group

is again becoming practice, as evidenced by human clinical trials [19–22] and the application

of poxvirus-based vaccines in veterinary and agricultural sectors, especially in global rabies prevention programs [23]. Although there are

cur-rently no licensed vaccines for human parasitic diseases, VV vectors have been used to prevent cystic hydatid disease in sheep [24]. Protection

against diverse animal viruses with economic implications has also been demonstrated. Within the swine industry, recombinant poxviruses have been used to immunize against the devastating effects of porcine reproductive and respiratory syndrome virus [25] and pseudorabies [26].

Recently, avian influenza vaccines for chickens that use live FPV recombinants expressing strain-specific antigens have been approved for use in several countries [27], joining the list of

FPV- and CPV-based veterinary vaccines that are commercially available [28].

Despite this progress, the sixty-four million dollar question remains – are poxviruses the Holy Grail in the fight against human diseases that have largely resisted concerted vaccine development efforts? To explore this question, we look at the role of poxvirus-based vaccines in two especially resistant conditions, malaria and HIV–AIDS. Despite overt dissimilarity in causa-tive pathogens, both diseases are characterized by therapeutic stalemates in which interventions are able to reduce adverse effects but not eliminate the pathogen. The end goal in these conditions is not sterilizing immunity, but rather manage-ment of pathogen load during the critical early stages of infection in an effort to delay disease onset, reduce debilitating symptoms and limit transmission. To achieve this goal, vaccination strategies focus on restricting the spread of the pathogen as the host immune system reacts or boosting the initial responses to infection.

‘Despite this progress, the sixty-four million dollar question remains – are poxviruses the Holy Grail in the fight

against human diseases that have largely resisted concerted vaccine

development efforts?’

The causative agent of malaria is a vector-borne, single-cell, eukaryotic parasite of the genus Plas-modium [29]. Vaccine development has been

hampered by the complexity of the malarial par-asite’s life cycle, which incorporates several dif-ferent replicative stages and forms. The parasite also exhibits the ability to replicate in terminally

differentiated erythrocytes lacking MHC-I expression, severely negating the impact of cell-mediated immune responses. Thus, acquired immunity develops slowly as the host attempts to mount a response against a constantly evolv-ing target without utilizevolv-ing its primary resources against intracellular pathogens. Owing to this complexity, multivalent subunit vaccines are the vaccine strategy of choice [30] but efforts in this

area have been limited by the need to identify protective rather than immunizing antigens and the continuing slow rate of host immune acqui-sition. Candidate antigens are also problematic as a group in that they are poorly immunogenic and possess complex structures that challenge stability and purification.

‘It can be argued that advances with recombinant poxvirus vector technologies continue to invigorate

the next generation of vaccines, particularly in such areas as

cancer immunotherapy’.

Poxvirus-based vectors have the potential to alleviate many of these concerns. For example, poxvirus vectors have been shown to enhance the immunogenicity of recombinant protein-based vaccines, possibly through enhanced immune responses against the vector itself [31]. Poxviruses

employ host protein production machinery to express the transgene product, thereby bypassing both the need for expensive production of puri-fied protein and difficulties associated with retaining complex structures. Furthermore, the poxviral genome supports incorporation of mul-tiple antigens from the different parasite forms within a single construct. This potential is best exemplified by NYVAC-Pf7, an attenuated VV-vector expressing seven different recombinant proteins, including the primary anti-invasive tar-gets merozoite protein-1 and circumsporozoite protein (CSP) [32,33]. Clinical trials with

NYVAC-pf7 demonstrated more effective immune responses than a comparable VV-based vector expressing only CSP and delayed onset of malaria symptoms, but did not provide complete protection. However, greater success with poxvi-rus vectors has been achieved using heterologous prime–boost regimens. For example, CD8+

T-cell responses capable of providing protection against sporozoite challenge were observed when a primary DNA vaccine against two antigens, CSP or trombospondin-related adhesive protein (TRAP), was followed by a poxvirus-based

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booster [34]. This finding translated into similar

reduced parasite loads in subsequent human tri-als using FPV and MVA expressing TRAP and several other antigens [35]. Recently, Phase II

tri-als have begun and interest in the potential inherent in co-administration of poxvirus vectors and DNA vaccines as a new protocol of immuni-zation continues [36].

Given the expense, poor accessibility and numerous adverse side effects associated with anti-HIV drug therapies, vaccines also represent the most cost-effective and practical control measure in HIV–AIDS. However, HIV shares with the malaria parasite the ability to rapidly colonize host cells in a manner that dramatically reduces the ability to mount an immune response against the pathogen. Analogous to the multiple forms of the plasmodium parasite, the HIV virion is also constantly mutating in response to immune pressure and inherent genetic variability, creating a moving target for the host immune system. Early proof-of-princi-ple studies in both human and nonhuman pri-mates suggested great promise in inducing an immune response against HIV with VV recom-binants expressing relevant viral envelope anti-gens [37,38], but quickly led to clinical trials that

failed to deliver on the promise of efficacy. However, these trials did reveal that a prime–boost regimen greatly improved the extent and duration of immune responses over virus-vectored antigen alone [39]. More recent

studies using nonenvelope HIV antigens in combination with antivirus drugs or DNA vac-cines have confirmed this finding and suggested the potential for therapeutic vaccination [40–42].

The scope and breadth of potential vaccine can-didates developed and evaluated in the last few years are far too numerous for this editorial and have been reviewed elsewhere. However, among the more novel strategies being investigated is the incorporation of MVA–HIV envelope recombinants as orally available liposomal com-plexes. In mice, these complexes have been found to enhance envelope-specific cellular and humoral immune responses [43]. In addition,

several cytokines, such as IL-15 and IFN-γ, have been incorporated into anti-HIV poxvirus vac-cines in an attempt to promote TH1-specific responses and induce memory [44,45].

Safety concerns, and the finding in the early HIV trials that pre-existing immunity to VV decreased the efficacy of VV-based vaccines for HIV [46], have focused recent efforts on the use

of attenuated poxvirus species. Of note are the

multisubunit MVA and NYVAC recombinants expressing HIV gag-pol-nef antigens in addition to the viral envelope genes that have been shown to consistently boost both CD4+ and CD8+

T-cell responses in HIV-1-infected patients [47,48].

In addition, novel inert poxvirus vectors have been developed in order to reduce the immune response against the vector itself, such as the highly attenuated VV substrain Dairen-I [49] and

VV strains lacking the B5 ectodomain against which many host immune responses are directed

[50].

‘As our understanding of the biology of poxviruses and the consequences of manipulating the poxviral genome expands, it seems logical that a human candidate

vaccine is on the horizon.’

As described above, the poxvirus vector plat-form affords multiple vaccination strategies with diverse potential applications in human and ani-mal disease. It can be argued that advances with recombinant poxvirus vector technologies con-tinue to invigorate the next generation of vac-cines, particularly in such areas as cancer immunotherapy [51]. The potential and

excite-ment that accompanied the initial proposal in 1982 to use poxvirus-based vaccines has sub-sided to some extent, but the field continues to be one of extensive interest and research efforts. Successes within veterinary medicine have shown that the theory supporting the use of poxvirus vaccines is sound. As our understand-ing of the biology of poxviruses and the conse-quences of manipulating the poxviral genome expands, it seems logical that a human candi-date vaccine is on the horizon. However, the biggest hurdle to overcome is likely never to be a scientific one. Rather, public perception and the fear of deliberately dosing oneself with a relative of an ancestral scourge will remain the greatest obstacle.

Financial & competing interests disclosure The authors have no relevant affiliations or financial involvement with any organization or entity with a finan-cial interest in or finanfinan-cial conflict with the subject matter or materials discussed in the manuscript. This includes employ-ment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties.

No writing assistance was utilized in the production of this manuscript.

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4

EDITORIAL

– Nino-Fong & Johnston

Future Virol. (2008) 3(2) future science groupfuture science group

Bibliography

1. Moss B: Poxviridae: In: The Virus and Their Replication. Knipe DM, Howley PM (Eds).

Lippincott Williams and Wilkins, Fields Virology, PA, USA 2849–2883 (2001).

2. Moroziewicz D, Kaufman HL: Gene therapy with poxvirus vectors. Curr. Opin. Mol. Ther. 7, 317–325 (2005).

3. Arlen PM, Kaufman HL, DiPaola RS: Pox viral vaccine approaches. Semin. Oncol. 32,

549–555 (2005).

4. Thorne SH, Hwang TH, Kirn DH: Vaccinia virus and oncolytic virotherapy of cancer. Curr. Opin. Mol. Ther. 7, 359–365

(2005).

5. Hakim AA, Lin PS, Wilczynski S, Nguyen K, Lynes B, Wakabayashi MT: Indications and efficacy of the human papillomavirus vaccine. Curr. Treat. Options Oncol. DOI:

10.1007/s11864-007-0050-0 (2008) (Epub ahead of print).

6. Boyle DB, Anderson MA, Amos R, Voysey R, Coupar BE: Construction of

recombinant Fowlpox viruses carrying multiple vaccine antigens and immunomodulatory molecules.

Biotechniques 37, 104–111 (2004).

7. Rocha CD, Caetano BC, Machado AV, Bruna-Romero O: Recombinant viruses as tools to induce protective cellular immunity against infectious diseases. Int. Microbiol. 7,

83–94 (2004).

8. Meyer H, Sutter G, Mayr A: Mapping of deletions in the genome of the highly attenuated Vaccinia virus MVA and their influence on virulence. J. Gen. Virol.

72(Pt 5), 1031–1038 (1991).

9. Tartaglia J, Perkus ME, Taylor J et al.:

NYVAC: a highly attenuated strain of Vaccinia virus. Virology 188, 217–232

(1992).

10. Plotkin SA, Cadoz M, Meignier B et al.:

The safety and use of canarypox vectored vaccines. Dev. Biol. Stand. 84, 165–170

(1995).

11. Skinner MA, Laidlaw SM, Eldaghayes I, Kaiser P, Cottingham MG: Fowlpox virus as a recombinant vaccine vector for use in mammals and poultry. Expert Rev. Vaccines

4, 63–76 (2005).

12. Rziha HJ, Henkel M, Cottone R, Meyer M, Dehio C, Buttner M: Parapoxviruses: potential alternative vectors for directing the immune response in permissive and non-permissive hosts. J. Biotechnol. 73, 235–242

(1999).

13. Black DN: Capripoxvirus-based multivaccines. Dev. Biol. Stand. 84,

179–182 (1995).

14. Kreijtz JH, Suezer Y, van Amerongen G et al.: Recombinant modified Vaccinia virus

Ankara-based vaccine induces protective immunity in mice against infection with influenza virus H5N1. J. Infect. Dis. 195,

1598–1606 (2007).

15. McShane H, Pathan AA, Sander CR et al.:

Recombinant modified Vaccinia virus Ankara expressing antigen 85A boosts BCG-primed and naturally acquired

antimycobacterial immunity in humans.

Nat. Med. 10, 1240–1244 (2004).

16. Miao J, Li X, Liu Z, Xue C, Bujard H, Cui L: Immune responses in mice induced by prime-boost schemes of the Plasmodium falciparum apical membrane antigen 1 (PfAMA1)-based DNA, protein and recombinant modified Vaccinia Ankara vaccines. Vaccine 24, 6187–6198 (2006).

17. Boursnell ME, Rutherford E, Hickling JK et al.: Construction and characterisation of a

recombinant Vaccinia virus expressing human papillomavirus proteins for immunotherapy of cervical cancer. Vaccine

14, 1485–1494 (1996).

18. Lindsey KR, Gritz L, Sherry R et al.:

Evaluation of prime/boost regimens using recombinant poxvirus/tyrosinase vaccines for the treatment of patients with metastatic melanoma. Clin. Cancer Res. 12, 2526–2537

(2006).

19. Arlen PM, Gulley JL: Therapeutic vaccines for prostate cancer: a review of clinical data.

Curr. Opin. Investig. Drugs 6, 592–596

(2005).

20. Meyer RG, Britten CM, Siepmann U et al.:

A Phase I vaccination study with tyrosinase in patients with stage II melanoma using recombinant modified Vaccinia virus Ankara (MVA-hTyr). Cancer Immunol. Immunother. 54, 453–467 (2005).

21. Bejon P, Mwacharo J, Kai O et al.: A

Phase 2b randomised trial of the candidate malaria vaccines FP9 ME-TRAP and MVA ME-TRAP among children in Kenya. PLoS Clin. Trials 1, e29 (2006).

22. Russell ND, Graham BS, Keefer MC et al.:

Phase 2 study of an HIV-1 canarypox vaccine (vCP1452) alone and in combination with rgp120: negative results fail to trigger a Phase 3 correlates trial.

J. Acquir. Immune Defic. Syndr. 44, 203–212

(2007).

23. Blancou J, Kieny MP, Lathe R et al.: Oral

vaccination of the fox against rabies using a live recombinant Vaccinia virus. Nature 322,

373–375 (1986).

24. Marsland BJ, Tisdall DJ, Heath DD, Mercer AA: Construction of a recombinant orf virus that expresses an Echinococcus granulosus

vaccine antigen from a novel genomic insertion site. Arch. Virol. 148, 555–562

(2003).

25. Shen G, Jin N, Ma M et al.: Immune

responses of pigs inoculated with a recombinant Fowlpox virus coexpressing GP5/GP3 of porcine reproductive and respiratory syndrome virus and swine IL-18.

Vaccine 5, 4193–4202 (2007).

26. Fischer T, Planz O, Stitz L, Rziha HJ: Novel recombinant parapoxvirus vectors induce protective humoral and cellular immunity against lethal herpesvirus challenge infection in mice. J. Virol. 77, 9312–9323 (2003).

27. Bublot M, Pritchard N, Swayne DE et al.:

Development and use of fowlpox vectored vaccines for avian influenza. Ann. NY Acad. Sci. 1081, 193–201 (2006).

28. Poulet H, Minke J, Pardo MC, Juillard V, Nordgren B, Audonnet JC: Development and registration of recombinant veterinary vaccines. The example of the canarypox vector platform. Vaccine 25, 5606–5612

(2007).

29. Tuteja R: Malaria – an overview. FEBS J.

274, 4670–4679 (2007).

30. Ballou WR, Arevalo-Herrera M, Carucci D

et al.: Update on the clinical development of

candidate malaria vaccines. Am. J. Trop. Med. Hyg. 71, 239–247 (2004).

31. Hutchings CL, Gilbert SC, Hill AV, Moore AC: Novel protein and poxvirus-based vaccine combinations for simultaneous induction of humoral and cell-mediated immunity. J. Immunol. 175, 599–606

(2005).

32. Ockenhouse CF, Sun PF, Lanar DE et al.:

Phase I/IIa safety, immunogenicity, and efficacy trial of NYVAC-Pf7, a pox-vectored, multiantigen, multistage vaccine candidate for Plasmodium falciparum

malaria. J. Infect. Dis. 177, 1664–1673

(1998).

33. Tine JA, Lanar DE, Smith DM et al.:

NYVAC-Pf7: a poxvirus-vectored, multiantigen, multistage vaccine candidate for Plasmodium falciparum malaria. Infect. Immun. 64, 3833–3844 (1996).

34. Webster DP, Dunachie S, Vuola JM et al.:

Enhanced T cell-mediated protection against malaria in human challenges by using the recombinant poxviruses FP9 and modified Vaccinia virus Ankara. Proc. Natl Acad. Sci. USA 102, 4836–4841 (2005).

35. McConkey SJ, Reece WH, Moorthy VS et al.: Enhanced T-cell immunogenicity of

plasmid DNA vaccines boosted by recombinant modified Vaccinia virus Ankara in humans. Nat. Med. 9, 729–735

(6)

Author Proof

36. Weiss WR, Kumar A, Jiang G et al.:

Protection of rhesus monkeys by a DNA prime/poxvirus boost malaria vaccine depends on optimal DNA priming and inclusion of blood stage antigens. PLoS ONE 2, E1063 (2007).

37. Cho MW, Kim YB, Lee MK et al.:

Polyvalent envelope glycoprotein vaccine elicits a broader neutralizing antibody response but is unable to provide sterilizing protection against heterologous

Simian/human immunodeficiency virus infection in pigtailed macaques. J. Virol. 75,

2224–2234 (2001).

38. Cooney EL, Collier AC, Greenberg PD et al.: Safety of and immunological response to

a recombinant Vaccinia virus vaccine expressing HIV envelope glycoprotein.

Lancet 337, 567–572 (1991).

39. Evans TG, Keefer MC, Weinhold KJ et al.:

A canarypox vaccine expressing multiple human immunodeficiency virus type 1 genes given alone or with rgp120 elicits broad and durable CD8+ cytotoxic

T lymphocyte responses in seronegative volunteers. J. Infect. Dis. 180, 290–298

(1999).

40. Goonetilleke N, Moore S, Dally L et al.:

Induction of multifunctional human immunodeficiency virus type 1 (HIV-1)-specific T cells capable of proliferation in healthy subjects by using a prime-boost regimen of DNA- and modified Vaccinia virus Ankara-vectored vaccines expressing HIV-1 Gag coupled to CD8+ T-cell

epitopes. J. Virol. 80, 4717–4728 (2006).

41. Shinoda K, Xin KQ, Kojima Y, Saha S, Okuda K, Okuda K: Robust HIV-specific immune responses were induced by DNA vaccine prime followed by attenuated recombinant Vaccinia virus (LC16m8

strain) boost. Clin. Immunol. 119, 32–37

(2006).

42. Harrer E, Bauerle M, Ferstl B et al.:

Therapeutic vaccination of HIV-1-infected patients on HAART with a recombinant HIV-1 nef-expressing MVA: safety, immunogenicity and influence on viral load during treatment interruption. Antivir. Ther.

10, 285–300 (2005).

43. Naito T, Kaneko Y, Kozbor D: Oral vaccination with modified Vaccinia virus Ankara attached covalently to TMPEG-modified cationic liposomes overcomes pre-existing poxvirus immunity from recombinant vaccinia immunization. J. Gen. Virol. 88, 61–70 (2007).

44. McKee HJ, T’sao PY, Vera M, Fortes P, Strayer DS: Durable cytotoxic immune responses against gp120 elicited by recombinant SV40 vectors encoding HIV-1 gp120 +/- IL-15. Genet. Vaccines Ther. 2, 10

(2004).

45. Giavedoni LD, Jones L, Gardner MB et al.:

Vaccinia virus recombinants expressing chimeric proteins of human

immunodeficiency virus and γinterferon are attenuated for nude mice. Proc. Natl Acad. Sci. USA 89, 3409–3413 (1992).

46. Belyakov IM, Moss B, Strober W, Berzofsky JA: Mucosal vaccination overcomes the barrier to recombinant vaccinia immunization caused by preexisting poxvirus immunity. Proc. Natl Acad. Sci. USA 96, 4512–4517 (1999).

47. Hanke T, McMichael AJ, Dorrell L: Clinical experience with plasmid DNA- and modified Vaccinia virus Ankara-vectored human immunodeficiency virus type 1 clade A vaccine focusing on T-cell induction. J. Gen. Virol. 88, 1–12 (2007).

48. Gomez CE, Najera JL, Jimenez EP et al.:

Head-to-head comparison on the

immunogenicity of two HIV/AIDS vaccine candidates based on the attenuated poxvirus strains MVA and NYVAC co-expressing in a single locus the HIV-1BX08 gp120 and HIV-1(IIIB) Gag-Pol-Nef proteins of clade B. Vaccine 25, 2863–2885 (2007).

49. Okamura T, Someya K, Matsuo K, Hasegawa A, Yamamoto N, Honda M: Recombinant vaccinia DIs expressing simian immunodeficiency virus gag and pol in mammalian cells induces efficient cellular immunity as a safe immunodeficiency virus vaccine candidate. Microbiol. Immunol. 50,

989–1000 (2006).

50. Viner KM, Girgis N, Kwak H, Isaacs SN: B5-deficient Vaccinia virus as a vaccine vector for the expression of a foreign antigen in vaccinia immune animals. Virology 361,

356–363 (2007).

51. Moingeon P: Recombinant cancer vaccines based on viral vectors. Dev. Biol. (Basel) 116,

117–122 (2004). Affiliations

• Rodolfo Nino-Fong

Institute for Nutrisciences and Health, National Research Council Canada, 550 University Avenue, Charlottetown, PE C1A 4P3, Canada

Tel.: +1 902 566 8007; Fax: +1 902 367 7539; rodolfo.nino-fong@nrc.ca • James B Johnston

Institute for Nutrisciences and Health, National Research Council Canada, 550 University Avenue, Charlottetown, PE C1A 4P3, Canada

Tel.: +1 902 566 8007; Fax: +1 902 367 7539; james.johnston@nrc.ca

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