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Effect of temperature on Pseudomonas phospholipid composition
Tatiana Kondakova, Nadine Merlet-Machour, Josselin Bodilis, Frédéric Dionnet, Marc Feuilloley, Nicole Orange, Hermann Heipieper, Cécile
Duclairoir Poc
To cite this version:
Tatiana Kondakova, Nadine Merlet-Machour, Josselin Bodilis, Frédéric Dionnet, Marc Feuilloley, et al.. Effect of temperature on Pseudomonas phospholipid composition. Journées de l’Ecole doctorale normande Biologie Intégrative, Santé, Environnement (EdN BISE, 18, 2015), Apr 2015, Caen, France.
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T. Kondakova1,3, N. Merlet-Machour2, J. Bodilis1, F. Dionnet3, M. Feuilloley1, N. Orange1, H.J. Heipieper4, C. Duclairoir Poc1
1 Laboratory of Microbiology Signals and Microenvironment EA 4312, Normandie Univ., Univ. Rouen, IRIB, 27000 Evreux, France
2 Team Modified to Surface and Interface Analysis (SIMA), UMR 6014 COBRA, Normandy Univ., Univ. Rouen, 55 rue St Germain, 27000 Evreux, France,
3 Aerothermic and Internal Combustion Engine Technological Research Centre (CERTAM), 1 rue Joseph Fourier, 76800 Saint Etienne du Rouvray, France,
4 Department of Environmental Biotechnology, UFZ Helmholtz Centre for Environmental Research, Permoserstr. 15, 04318 Leipzig, Germany.
Effect of temperature on Pseudomonas phospholipid composition
Introduction
Pseudomonas are present in all natural environments, and able to quickly adapt to environmental modifications. Physiologically, this adaptation depends essentially to the structure and organization of their envelope. The membrane functions are traditionally attributed to proteins that are immersed in the lipid bilayer, but lipids are not only a matrix to accommodate proteins, but play a major role in bacterial functioning. P. fluo- rescens is a psychrophilic bacterium commonly found in soil and water. Some of P. fluorescens members are able to growth at the human tem- perature, as members of the skin microbiota or behave as opportunistic pathogens.
Establish if the response to human temperature involves changes in the structure and the composition of P. fluorescens phospholipids at differ- ent phases of growth
Study of lipidomes of two P. fluorescens strains from different ecological niches: one clinical isolate MFN1032 and one airborne isolate MFAF76a Characterization of the phospholipid composition of P. fluorescens using an adapted HPTLC-MALDI-TOF MS Imaging tool
Conclusions Open questions
Objectifs
P. fluorescens is able to adapt to human temperature using two lipidic pathways:
1. the increasing of degree of fatty acid unsaturation controls the membrane fluidity
2. the increase of the synthesis of zwitteronic phos- pholipids results in the membrane charge changes.
1. The phosphatidylcholine role in Pseudomonas adaptation to human temperature
2. The new lphospholipid class PAL complet identi- fication
1. Adaptation of P. fluorescens to human temperature by membrane phospholipid modifications
0,00 0,20 0,40 0,60 0,80 1,00 1,20 1,40 1,60 1,80 2,00
exponential phase stationary phase exponential phase stationary phase
28°C 37°C
Degree of saturation
MFAF76a MFN1032
***
***
*
MFAF76a MFN1032
Stationary growth phase Stationary growth phase
Rf
MFAF76a MFN1032
Exponential growth phase
MFAF76a MFN1032
28°C
Exponential growth phase
MFAF76a MFN1032
37°C
5 mm
0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Phosphatidylcholine Phosphoaminolipid
Phosphatidylethanolamine Viscosinamide
Phosphatidylglycerol
Temperature and phospholipid composition Temperature and degree of fatty acids’ saturation
At 37°C the both strains product more zwitteronic phospholipids, like phosphatidyléthanolamine and phosphatidylcholine
To adapt to human temperature the degree of fatty acids’
saturation increases in both P. fluorescens strains
P. fluorescens adapts to temperature changes via membrane phospholipid modification.
1. The overproduction of zwitteronic phospholipids allows the changes of membrane charge
2. The phosphatidylcholine overproduction may be involved in P.
fluorescens - host interactions.
3. The increasing of degree of fatty acids’ saturation allows P. fluo- rescens to keep the optimal membrane fluidity
2. New phospholipid class identification
927.5
[PAL1+Na]+
356.1
[HG+H]+
378.1
[HG+Na]+ 927.5[PAL1+Na]
+
905.5
[PAL+H]+
648.5
[PAL-257+H]+ [PAL-257+Na]670.6 +
**
927.5
953.5 941.5
[PAL1+Na]+
[PAL2+Na]+
[PAL3+Na]+
m/z
