HAL Id: hal-01363593
https://hal.archives-ouvertes.fr/hal-01363593
Submitted on 10 Sep 2016
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Purification of recombinant human and Drosophila septin hexamers for TIRF assays of actin-septin filament
assembly
Manos Mavrakis, Tsai Feng-Ching, Gijsje H. Koenderink
To cite this version:
Manos Mavrakis, Tsai Feng-Ching, Gijsje H. Koenderink. Purification of recombinant human and
Drosophila septin hexamers for TIRF assays of actin-septin filament assembly. Methods in Cell Biol-
ogy, Elsevier, 2016, Septins, 136, pp.199. �10.1016/bs.mcb.2016.03.020�. �hal-01363593�
Running title:
In vitro reconstitution of actin-septin filament assembly Title:
Purification of recombinant human and Drosophila septin hexamers for TIRF assays of actin-septin filament assembly
Manos Mavrakis
1*, Feng-Ching Tsai
2, 3and Gijsje H. Koenderink
2*
1
Aix Marseille Université, CNRS, Centrale Marseille, Institut Fresnel, UMR 7249, Faculté des Sciences Saint-Jérôme, 13013 Marseille, France
2
FOM Institute AMOLF, Science Park 104, 1098 XG Amsterdam, The Netherlands
3