• Aucun résultat trouvé

Table of Contents I.

N/A
N/A
Info
Télécharger
Protected

Academic year: 2021

Partager "Table of Contents I."

Copied!
1
0
0

Chargement.... (Voir le texte intégral maintenant)

Texte intégral

(1)

Table of Contents

I. Introduction ... 1

A. Cancer ... 1

B. Chemotherapy and other treatments ... 3

C. Individual therapy... 5

D. Membrane transporters: Drug efflux or influx ... 6

1. ATP binding cassette (ABC) transporters ... 7

2. The solute carrier (SLC) transporters ... 15

E. The solute carrier 22 family (SLC22) ... 16

1. The OCT subfamily transporters in the cancer ... 20

2. The OCTN subfamily transporters in the cancer ... 21

3. The OAT subfamily transporters in the cancer ... 21

II. Aim and strategy ... 25

III. Results and discussion ... 29

A. Overexpression system ... 29 1. Construct design ... 30 2. Cell line ... 31 3. Transfection agent ... 31 4. Expression level ... 32 5. viability assay ... 33

6. Expression and cell viability assays on cells transfected transiently ... 35

7. Expression and cell viability assays on stable cell lines ... 39

8. Conclusion ... 53

B. Downregulation system ... 54

1. Endogenous SLC22A18 expression cell line ... 54

2. Knockdown cell line ... 56

3. Knockout cell line ... 63

4. Conclusion ... 66

IV. Conclusion and perspectives ... 69

V. Methods ... 73

A. Molecular biology ... 73

B. Cellular biology ... 79

VI. Annex ... 89

A. Cancer resistance mechanism ... 89

B. SLC22 members ... 90

C. Drugs selected ... 93

D. Others antimitotic drugs ... 94

Références

Télécharger maintenant ( PDF - 1 Page - 50.51 KB )

Documents relatifs

β-escin selectively targets the glioblastoma-initiating cell population and reduces cell viability

In order to confirm that β-escin selectively targets GIC, a dose response was performed in the differentiated human primary GBM cell lines, U87, LN229, as well as in GIC#1 and

Review of Prognostic Expression Markers for Clear Cell Renal Cell Carcinoma

Loss of BAP1 protein expression is an independent marker of poor prognosis in patients with low-risk clear cell renal cell carcinoma. Minardi D, Lucarini G, Milanese G, Di Primio

Genome-wide expression profiling and functional characterization of SCA28 lymphoblastoid cell lines reveal impairment in cell growth and activation of apoptotic pathways.

We performed a whole genome expression profiling using lymphoblastoid cell lines (LCLs) from four SCA28 patients and six unrelated healthy controls matched for sex and age..

Host cell-specific protein expression in

ruminantium proteins encoded by the map1 multigene family, expressed in vitro in 31.. bovine endothelial and tick

"FABuleux objets"

Vous avez été par moment créateurs, parfois dans un atelier conventionnel d'activités créatrices manuelles, parfois dans un FabLab, tout cela pour vous faire réfléchir à ce qui

Expression of Rod-Derived Cone Viability Factor: Dual Role of CRX in Regulating Promoter Activity and Cell-Type Specificity.

We next investigated the cell type specificity of the various promoter regions by determining the spatial distribution of GFP and RFP positive cells in slices of retinal explants 5

Premières Informations 2007-38.2 - L'emploi salarié au deuxième trimestre 2007 (09-38.2-5.PDF, 168.87 Ko)

Une correction de biais est alors nécessaire : elle est obtenue, pour chaque secteur d'activité (en NES16 et NES36), par un étalonnage de l'indicateur sur les estimations

Dares Indicateurs 2017-051 - L’emploi intérimaire diminue au 1er trimestre 2017 (PDF, 637.64 Ko)

Historiquement, l’estimation d’emploi intérimaire reposait sur les « relevés mensuels de mission » (RMM) déposés chaque mois par les agences d’intérim auprès de Pôle