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Effects of lipids on membrane order level by using GUVs

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HAL Id: hal-02803570

https://hal.inrae.fr/hal-02803570

Submitted on 5 Jun 2020

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Effects of lipids on membrane order level by using GUVs

Kévin Grosjean, Patricia Gerbeau-Pissot, Christophe Der, Françoise Simon Plas

To cite this version:

Kévin Grosjean, Patricia Gerbeau-Pissot, Christophe Der, Françoise Simon Plas. Effects of lipids on

membrane order level by using GUVs. Ecole Regulation et Dynamique des Membranes Biologiques,

May 2013, Porquerolles, France. �hal-02803570�

(2)

Résumés pour "Ecole Régulation et Dynamique des Membranes Biologiques." Porquerolles, France, 27-30 mai 2013

Effects of lipids on membrane order level by using GUVs

Kevin Grosjean

1

, Patricia Gerbeau-Pissot

1

, Christophe Der

1

and Françoise Simon-Plas

2

1- Université de Bourgogne, UMR 1347 Agroécologie, ERL 6300 CNRS, BP 86510, F-21000 Dijon, France

2- INRA, UMR 1347 Agroécologie, ERL 6300 CNRS, BP 86510, F-21000 Dijon, France

A large set of data, obtained by various methods have revealed that biological membranes are laterally subdivided into domains enriched in specific lipid and protein components. The use of biophysical data, supplemented by analysis of synthetic membrane and plasma membrane (PM) with labeled lipids or environmentally sensitive fluorescent probe allows to describe different structural properties of the membrane.

Several possible states of membrane were thus identified: one called “liquid ordered” or lo, crystalline structure where the lipid chains are tight; the second one “the liquid disordered” or ld, fluid phase where the lipid diffusion is high; finally there is the “gel” phase, also called solid lamellar.

To study the spatial distribution of the different phases, we decided to analyze the plane

surface of the membrane. We developed a methodology using multispectral confocal

microscopy associated with the di-4-ANEPPDHQ probe which provides information about

the order level of the membrane in which it is integrated. We produced model membrane

vesicles, precisely giant unilamellar vesicles (GUVS) with particular lipidic composition, in

order to obtain vesicles with lo, ld or lo/ld associated phases and labeled them with the

fluorescent dye. This allowed us to record pictures of large membrane surface (5 to 15 µm

2

),

necessary to describe the spatial organization of the membrane order level. The dependence of

the size and distribution of areas of different order level on the composition of GUVs was

examined.

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