Increasing uncoupling protein-2 in pancreatic beta cells does not alter glucose-induced insulin secretion but decreases production of reactive oxygen species

Our results show that the defec- tive glucose-induced insulin secretion observed after H 2 O 2 treatment correlates with altered mitochondrial activation seen as a loss of

Irrespective of the glutamate levels measured in glucose- stimulated cells, the principal observation is that glutamate directly stimulates insulin exocytosis in permeabilised cells

F IG. Induction of WT-HNF1␣ and DN-HNF1␣ regulates the HNF1␣ mRNA expression and HNF1␣ promoter luciferase activity through altered HNF4 ␣ binding. A, for EMSA, the

Cells overexpressing GAD follow- ing AdCA-GAD65 infection and stimulation with high glucose could therefore theoretically release significant amounts of GABA with a possible

Here we demonstrate that high concentrations of glucose induce IL-1 β production and secretion in human β cells, leading to Fas receptor upregulation, NF-κB activation, β

Although intracellular glutamate potentiates insulin secretion, extracellular glutamate may activate ionotropic receptors.. As a consequence of such activation, insulin exocytosis

In parallel, CART silencing decreased synthesis of insulin and expression of genes encoding proteins important for exocytosis, glucose sensing, and insulin processing, likely as

To study the role of calpain on ␤ -cell function, ␤ -cells were plated on PLL or 804G matrix and preincubated for 24 h with calpeptin, and short-term insulin secretion was then