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IL-8 expression and its possible relationship with estrogen-receptor-negative status of breast cancer cells.

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HAL Id: inserm-00143840

https://www.hal.inserm.fr/inserm-00143840

Submitted on 27 Apr 2007

HAL is a multi-disciplinary open access archive for the deposit and dissemination of sci-entific research documents, whether they are pub-lished or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers.

L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

IL-8 expression and its possible relationship with

estrogen-receptor-negative status of breast cancer cells.

Ariane Freund, Corine Chauveau, Jean-Paul Brouillet, Annick Lucas,

Matthieu Lacroix, Anne Licznar, Françoise Vignon, Gwendal Lazennec

To cite this version:

Ariane Freund, Corine Chauveau, Jean-Paul Brouillet, Annick Lucas, Matthieu Lacroix, et al.. IL-8 expression and its possible relationship with estrogen-receptor-negative status of breast cancer cells.. Oncogene, Nature Publishing Group, 2003, 22 (2), pp.256-65. �10.1038/sj.onc.1206113�. �inserm-00143840�

(2)

M IL8

C

D

MDCNF precursor IL-8 (h) 0 4 12 48 E2 pS2 E2 (h) 0 48 IL-8 0 4 12 48 28S

A

B

MCF-7 MDA-MB-231 0 48 MCF-7 231 1.6 1 0.5 kb

(3)

Fig. 2

IL-8

pS2

C E2

C E2

28S

C E2

BT-474

C E2

C E2

CAMA-1

IL-8

pS2

28S

C E2

ZR-75-1

(4)

0 2 4 6 8 225 336

BT-20 MDA-MB- MDA-MB- MDA-MB- MDA-MB- SKBR-3

S e c reted IL-8

(

ng/ml/ 48h/Mill ion cells)

A

ER-231 435 436 468 0 2 4 6 8 10 BT-474 CAMA-1 MCF-7 T47D ZR-75-1

B

ER+

S e c reted IL-8

(

ng/ml/ 48h/Mill ion cells) 6 45

C

ER+

(5)

Fig. 3

Ad5 Ad-hERα Ad-hERβ

0 10 20 30 40 50 S e c reted IL-8

(

ng/ml/ 48h/Mill ion cells) 0 1 2 3 4 5 6 7 8 BT-20

MDA-MB-231 MDA-MB-435 MDA-MB-436 MDA-MB-468

SK BR-3 BT -474 CAMA-1 MCF-7 T 47D ZR-7 5-1 Hel a PE O14 BG1 PE O4

F

Arbitr a ry U nits

E

0 1 2 3 4 5 6 7 8 BT 20

MDA-MB-231 MDA-MB-435 MDA-MB-436 MDA-MB-468

SKBR3 BT -474 CAMA-1 MCF-7 T 47D Z R -75-1 Hela PEO14 PEO4 BG1 Fold In duc tio n

(6)

(arbitrar

y

units)

40

60

80

100

120

IL-8

rS9

ER

α

1

2

3

4

5

6

7

8

9

10 11

12 13 14

ER (fmol/mg) PR (fmol/mg) 1 5 0.6 2 0 6.2 3 2.7 22.6 4 2.9 6.5 5 1.1 12 6 4.3 0.6 7 2.2 2.4 8 141.2 256.1

C

B

n=7

ER

β

(7)

Fig. 5

BT20 MDA-MB-231 MDA-MB-435 MDA-MB-436 MDA-MB-468 SKBR3 BT-474 CAMA-1 MCF-7 T47D ZR-75-1

ER+

Hela PEO14 BG1 PEO4

ER+

ER-% of i

n

vasi

o

n

% of i

n

vasi

o

n

% of i

n

vasi

o

n

A

C

B

ER-0 5 10 15 20 0 5 10 15 20 25 0 5 10 15 20 25

(8)

C

IL-8

AS

Re la tiv e inv a sion ( % of c ontrol) 0 50 100 150 200

A

D

B

MDA-MB-435

MDA-MB-231 MDA-MB-436 MDA-MB-468 SK-BR-3 BT

-474 CAMA-1 MCF-7 T-47D ZR-7 5 M CXCR2 rS9 0 100 200 300 400

C

IL-8

Re la tiv e inv a sion ( % of c ontrol)

C

0 25 50 75 100

C

IL8 AS

Figure

Fig. 2IL-8pS2C E2C E228SC E2BT-474C E2C E2CAMA-1IL-8pS228SC E2ZR-75-1
Fig. 5BT20MDA-MB-231MDA-MB-435MDA-MB-436MDA-MB-468SKBR3BT-474CAMA-1MCF-7T47DZR-75-1ER+

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