Root system structure

(1)

Root system structure

A. Audebert

2 nd

_{Global Rice Phenotyping Network Worshop}

(2)

Rhizoscope :

A phenotyping system for root dynamics

Objective : Adult plants phenotyping

Principle : Mixed hydroponic system with

glass beads as inert substrate

• Transparent Plexiglas boxes (50 cm x 20

cm x 2 cm) with a grid of nails

• Filled with 1.5 mm diameter glass

beads = inert substrate providing

mechanical resistance.

•Automatic control of pH and

temperature of the nutrient solution

• Medium throughput system: 4 tanks

holding each 48 plants (192 plants)

• Plants up to 30 days old

(3)

What traits can be monitored?

• Manually or through image analysis:

– Shoot traits

• Leaf length; number of tillers per

plant; leaf number; shoot biomass;

– Root traits:

• Maximum crown root depth; root

mass at different depths; crown root

number at depth; root cone angle

– Root /shoot ratio; root mass per tiller

• Fluorescence microscope on crown root

tips

– Root diameter; stele area; nb of xylem

vessels; diameter of xylem vessels; nb of

mesodermis layers

with Volocity

with Image J

(4)

Phenotyping experiments

• 2011 and 2012

• Orytage and Grips funds

• Japonica and Indica panels (172 varieties phenotyped)

• 2 checks varieties (IR64 and Azucena) replicated in each tank

• 2 replications in time

• 30 days growth

• Traits

– Shoot biomass

– Root cone angle

– Maximum root depth

– Root nb at 30 cm depth

– Root biomass

• 0-20

• 20-30

• 30-50

– Anatomy

• Root tip

RB020

RB2030

RB30+

Depth

(5)

Rhizoscope platform : Some results

(6)

TAICHUNG NATIVE 1

S 624

RTS 14

ZALCHA

IR64

CARREON

Rhizoscope platform : Some results

(7)

Rhizoscope platform : Some results

IR64

AZUCENA

IR64

AZUCENA

0 0.05 0.1 0.15 0.2 0.25 20 30 40 50 60 70

Fr

é

que

nce

Root depth

0 0.05 0.1 0.15 0.2 0.25 200 300 400 500 600 700

Fr

é

que

nce

Root diameter

0 0.05 0.1 0.15 0.2 0.25 0.3 0 0.2 0.4 0.6

Fr

é

que

nce

Root biomass

AZUCENA

IR64

Col 2

Root/Shoot dw ratio

0,10

0,15

0,20

0,25

Number o f genot y pes

0

5

10

15

20

25

30 SD = 0.0105

Root depth (cm)

20 25 30 35 40 45 50 55 60 65

Num ber of gen oty pes

0

5

10

15

20

25

30 SD = 6.24

Root angle (°)

30

40

50

60

70

80

90 100 110

Numb er of genot y pes

0

5

10

15

20

25

30 SD = 7.04

Indica panel

japonica panel

(8)

Sub-population 2 :

Improved indicas

from Africa and

Asia

Sub-population 3 :

Traditional Indicas

from Asia

Sub-population 1 :

Indicas from

Madagascar

Diversity tree

established from 25

Markers

How explain the indicas diversity ?

Available information for theses varieties: Type (traditional or improved);

Geographical origin (large region); Genetic group

(9)

Sub-population 2 :

Japonicas from

Africa and South

America

Sub-population 1 :

Japonicas from

Asia

Diversity tree

established from 25

Markers

Available information for theses varieties: Type (traditional or improved);

Geographical origin (large region); Genetic group

(10)

Factorial analysis: Axes 1 / 2

93-11 APO APURA ARC15872 ASD 1 AZUCENA B6144-M R-6-0-0 BADKALAM KATI BAGUAM ON 14 BALA BENGALY 389 BG90-2 BH2 BODOM ANO BOTOHAVANA 139 BOTRA M AITSO BOTRIKELY BOTRY 731 BOUAKE 189 BR24 C4 63G CARREON CERE AIR CHAU CHERIVIRUPPU CHIEM CHANH CHITRAJ CICA 8 DA9 DE ABRIL DHOLA AM AN DIWANI DULAR ELONI FANDRAPOTSY 104 FEDEARROZ 2000 FEDEARROZ 50 FR13A GAJPATI GAM BIAKA GAM BIAKA KOKOUM

GIE 57

GOGO LEM PAK

GOPAL GWA NGASEIN HASAN SERAI IM 16 IR2006-P12-12-2-2 IR2307-247-2-2-3 IR5 IR50 IR52 IR55419-04 IR60 IR64 IR72 IRAT 121 JAM AJIGI

JC120 JC91 JENGAR KALILA M ENA KALINGA III KARASUKARA SURANKASU KARKATI 87 KASALATH KATI KAW LUYOENG

KHAO DAWK M ALI 105

KIANG CHOU CHIU

KITRANA 1890 KITRANA 508 LAL AM AN LOHAM BITRO 224 M ADINIKA 1329 M AKALIOKA 34 M ALADY M AM ORIAKA 114 M ENAHODITRA 1234 M TU 9 N 22 NAM SA GUI 19 NIONOKA NONA BOKRA ORYZICA LLANOS 5 ORYZICA SABANA 10 PA TOU HUNG PAPPAKU

PCT11\0\0\2,BO\1>55-1-3 PEH KUH PEH KUH TSAO TU

PELITA JANGGUT PETA PIN TAWNG POKKALI_1 POPOT PTB 25 PURBIA RATHAL 2 RATHUWEE RAY JAZAYKAYZ RAY NABJA ROJOFOTSY 693 ROJOKELY ROJOM ENA 1034 ROJOM ENA B48 RTS 12 RTS 14 RTS 4 RTS 5 S 624 SAHEL 108 SAHEL 159 SAHELIKA SALUM PIKIT SAO SEBOTA 65 SHORT GRAIN SOM CAU 70A

SWARNA TAICHUNG NATIVE 1 TAM CAU 9 A TELIM ANI TELOVOLANA 177 TETEP THAPACHINIYA THIERNO BANDE TOKAM BANY 663 TOKAM BANY 669 TSAKA TSIPALA 1231 TSIPALA FOTSY 1883 TSIPALA M ENA 626

UPL RI-7 VANDANA

VARY M ADINIKA 3494 VARY VATO 154

VARY VATO 462

VATO M ATSOAM ALONA

WAB 706-3-4-K4-KB-1

WAS105-B-IDSA-B-WAS2-1-WAS169-B-B-4-2-1

WAS181-B-6-3 WAS183-B-6-2-3 WAS182-B-1-1 WAS197-B-6-3-11 WAS20-B-B-1-2-2 WAS200-B-B-1-1-1 WAS202-B-B-1-1-2 WAS207-B-B-3-1-1 WAS208-B-B-5-1-1-3 WAS30-11-4-6-2-2-1 WAS49-B-B-9-1-4-2 WAS50-B-B-24-4-2-1 WAS55-B-B-2-1-2-5 WAS57-B-B-3-1-4-6 WAS62-B-B-17-1-1-3 WASSA WAY RAREM ZALCHA