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A contribution to the genetic mapping of resistance to the cotton blue disease using SSR markers

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Academic year: 2021

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IRGHUM (Sorghum l';Magalhâes,N2

1de Viçosa. 2Embrapa

19ram were evaluated ine BTx623, Tx430, SC283 andATFI4B, ldepending upon the with the u ceptible wilh approximately ce to each of the nine 1dicaled that the lines ~L-3and ARG-l had 'L-3 and ARG-I have ,nts were identilied in Overall, the results of e varied between two o s between BTx623 pattern ob erved for ge ting that BTx623 he coincidence of the l-te t, ugge ted that,

10two races whereas non genes conferring :e can be stacked for rogram. These genc

!to for u c in marker

GA061 A CONTRIBUTIO TO THE GENETIC MAPPI G OF RESISTANCE TO THE COT-TO BLUE DlSEASE U ING S R MARKERS

Cazé, ALRI.2; Oliveira, T 1.2; Hoffmann, LV'; Barroso, PAV'; Pires,

P;

chu ter,

13;

Giband, MI.4

'Laborat6rio de Biotecnologia, Embrapa Aigodâo. 2Departamento de Biologia, Universidade Estadual da Paraiba. JCooperativa Central de Pesquisa Agricola (COODETEC). 4Centre de Coopération Internationale en Recherche Agronomique pour

le Developpement - Cl RAD-BIO , UMR DAP, Montpellier, França analuizacaze@gmail.com

Key word : Cotton, Cotton Blue Disea e, SSR,Gossypium hir. ulum, Gos ypium barbadense One of the principal problems in the major cotton producing area of Brazil is the occurrence of disease ,amongst which the Cotton Blue Di ease ha a major impact. The ymptom include a decrease in intemode length, the reduction in plant, leaf and boll ize, a clearing of the leaf veins that appear darker and curved downward . The disea e i cau ed by a Luteovirus, the Cotton Leafroll Dwarf Virus (CLDV) that is transmitted by aphids. The control of the disease relies on the control to a low level of the aphid vector, but mo t importantly on the availability to growers ofre i tant varieties. Resistance to the virus i inherited as a monogenic and dominant character. Screening germpla m for Blue Disea e resi tance under naatural conditions in the field is complicated by the fact that aphid infestation does not occur homogenously, which results in the possibility of escape occurring. Thu the identification of PCR-ba ed molecular markers linked to Blue Disease resistance would be of great help to the cotton breeders in their efforts to breed disease resi tant varietie .ln order to identify such molecular markers linked to the resi -tance gene, an interspecific(Gossypium hirsulumvar. DeJtaOpal X G.barbadenseMTI21) F2 mapping population wa con tructed. The parental genotypes were cho en such as the resistance stems from a cultivated cultivar while maximizing marker polymorphism through the use of a susceptible G.barbaden eaccession. ixty-two individual plants of the F2population were used for DNA extraction and phenotyping for their re ponse to virus inoculation, which was achieved under controlled conditions in the greenhou e by tran ferring infectious aphid to the plantlet . Micro atellite markers were chosen for their ability to produ e clear amplification products and bands that are polymorphie between the two parental accessions. Results of the phenotyping confirm the impIe inhcritance - a single dominant gene - of the resistance in our population, thu validating the mapping population aS a tool for marker identification. Genotyping results showed that a significant number of markers (17%) howed a distorted segregation; nevertheless, this level of di tortion of segregation i similar to that observed in other similar cotton inter pecific mapping populations. Put together, thcse results show that the pre ent mapping population is adequate for the identification ofmolecular markers linked to Cotton Blue Di ease re istance. Supported by: CNPq

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