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The functional costs and benefits of dietary restriction in Drosophila

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Supplementary Material

The following supplementary material is available for this article:

Fig. S1 Effect of diet on age-related changes in starvation resistance. Age of flies was (A) 4 days, (B) 20 days, (C) 33 days, and (D) 47 days. Before the experiment, the sucrose and yeast concentration ranged from 7% (white symbols) to 11% (gray symbols) to 16% (black symbols). During the experiment, flies were kept on either a sucrose solution (control: circles) or water (treatment: triangles).

Fig. S2 Effect of diet on age-related changes in oxidative stress resistance. Age of flies was (A) 6 days, (B) 19 days, (C) 34 days, and (D) 47 days. Before the experiment, the sucrose and yeast concentration ranged from 7% (white symbols) to 11% (gray symbols) to 16% (black symbols). During the experiment, flies were kept on either a sucrose solution (control: circles) or a sucrose solution with paraquat (treatment: triangles).

Fig. S3 Effect of diet on immunosenescence. Age of flies was (A) 7 days, (B) 20 days, (C) 34 days, and (D) 48 days. The sucrose and yeast concentration ranged from 7% (white symbols) to 11%

(gray symbols) to 16% (black symbols). Flies were infected with either sterile broth (control: circles) or bacteria (treatment: triangles).

Bacteria were either Gram-negative Pseudomonas aeruginosa (left panels) or Gram-positive Lactococcus lactis (right panels).

Fig. S4 Effect of diet on age-related changes in cold-stress resilience. Age of flies was (A) 5 days, (B) 18 days, (C) 33 days, and (D) 43 days. The sucrose and yeast concentration ranged from 7% (white symbols) to 11% (gray symbols) to 16% (black symbols).

Table S1 Life tables for data of Fig. 1, S1, S2, S3 and S4.

This material is available as part of the online article from:

http://www.blackwell-synergy.com/doi/abs/10.1111/j.1474- 9726.2006.00261.x

(This link will take you to the article abstract).

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Fig. S2 Oxidative stress

0 0.2 0.4 0.6 0.8 1

0 24 48 72 96 120

Time to death (hours)

Proport ion alive

A

0 0.2 0.4 0.6 0.8 1

0 24 48 72

Time to death (hours)

Proport ion alive

B

0 0.2 0.4 0.6 0.8 1

0 12 24 36

Time to death (hours)

Proport ion alive

C

0 0.2 0.4 0.6 0.8 1

0 12 24 36

Time to death (hours)

Proport ion alive

D

(3)

Fig. S3 Immunity

0 0.2 0.4 0.6 0.8 1

0 12 24 36 48

Time to death (hours)

Proport ion alive

A

2

0 0.2 0.4 0.6 0.8 1

0 12 24 36 48

Time to death (hours)

Proport ion alive

B

2

0 0.2 0.4 0.6 0.8 1

0 12 24 36 48

Time to death (hours)

Proport ion alive

C

2

0 0.2 0.4 0.6 0.8 1

0 12 24 36 48

Time to death (hours)

Proport ion alive

D

2

0

0.2 0.4 0.6 0.8 1

0 12 24 36 48

Time to death (hours)

Proport ion alive

A

1

0 0.2 0.4 0.6 0.8 1

0 12 24 36 48

Time to death (hours)

Proport ion alive

B

1

0 0.2 0.4 0.6 0.8 1

0 12 24 36 48

Time to death (hours)

Proport ion alive

C

1

0 0.2 0.4 0.6 0.8 1

0 12 24 36 48

Time to death (hours)

Proport ion alive

D

1

(4)

0 0.2 0.4 0.6 0.8 1

0 10 20 30 40

Recovery time (min)

Proportion recovered

A

0 0.2 0.4 0.6 0.8 1

0 10 20 30 40

Recovery time (min)

Proportion recovered

B

0 0.2 0.4 0.6 0.8 1

0 10 20 30 40

Recovery time (min)

Proportion recovered

C

0 0.2 0.4 0.6 0.8 1

0 10 20 30 40

Recovery time (min)

Proportion recovered

D

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Fig. S1 Starvation

0 0.2 0.4 0.6 0.8 1

0 1 2 3 4 5

Time to death (days)

Proport ion alive

A

0 0.2 0.4 0.6 0.8 1

0 1 2 3 4 5

Time to death (days)

Proport ion alive

B

0 0.2 0.4 0.6 0.8 1

0 1 2 3 4 5

Time to death (days)

Proport ion alive

C

0 0.2 0.4 0.6 0.8 1

0 1 2 3 4 5

Time to death (days)

Proport ion alive

D

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age diet treatment vial x n(x) d(x) c(x)

4 7% control 1 0 15 0 0

4 7% control 1 12 15 0 0

4 7% control 1 18 15 0 0

4 7% control 1 24 15 0 0

4 7% control 1 26 15 0 0

4 7% control 1 28 15 0 0

4 7% control 1 30 15 0 0

4 7% control 1 32 15 0 0

4 7% control 1 34 15 0 0

4 7% control 1 36 15 0 0

4 7% control 1 38 15 0 0

4 7% control 1 40 15 0 0

4 7% control 1 42 15 0 0

4 7% control 1 43 15 0 0

4 7% control 1 44 15 0 0

4 7% control 1 46 15 0 0

4 7% control 1 48 15 0 0

4 7% control 1 50 15 0 0

4 7% control 1 52 15 0 0

4 7% control 1 54 15 0 0

4 7% control 1 56 15 0 0

4 7% control 1 58 15 0 0

4 7% control 1 60 15 0 0

4 7% control 1 62 15 0 0

4 7% control 1 64 15 0 0

4 7% control 1 66 15 0 0

4 7% control 1 68 15 0 0

4 7% control 1 70 15 0 0

4 7% control 1 72 15 0 0

4 7% control 1 74 15 0 0

4 7% control 1 78 15 0 0

4 7% control 1 80 15 0 0

4 7% control 1 82 15 0 0

4 7% control 1 84 15 0 0

4 7% control 1 86 15 0 0

4 7% control 1 88 15 0 0

4 7% control 1 90 15 0 0

4 7% control 1 92 15 0 0

4 7% control 1 94 15 0 0

4 7% control 1 96 15 0 0

4 7% control 1 100 15 0 0

4 7% control 1 104 15 0 0

4 7% control 1 106 15 0 0

4 7% control 1 108 15 0 0

4 7% control 1 110 15 0 0

4 7% control 1 112 15 0 15

4 7% control 1

4 7% control 1

4 7% control 1

4 7% control 1

4 7% control 1

4 7% starved 1 0 17 0 0

4 7% starved 1 12 17 0 0

4 7% starved 1 18 17 0 0

(7)

4 7% starved 1 26 17 0 0

4 7% starved 1 28 17 0 0

4 7% starved 1 30 17 0 0

4 7% starved 1 32 17 1 0

4 7% starved 1 34 16 0 0

4 7% starved 1 36 16 1 0

4 7% starved 1 38 15 1 0

4 7% starved 1 40 14 0 0

4 7% starved 1 42 14 4 0

4 7% starved 1 43 10 0 0

4 7% starved 1 44 10 0 0

4 7% starved 1 46 10 0 0

4 7% starved 1 48 10 0 0

4 7% starved 1 50 10 0 0

4 7% starved 1 52 10 1 0

4 7% starved 1 54 9 2 0

4 7% starved 1 56 7 0 0

4 7% starved 1 58 7 0 0

4 7% starved 1 60 7 0 0

4 7% starved 1 62 7 0 0

4 7% starved 1 64 7 1 0

4 7% starved 1 66 6 0 0

4 7% starved 1 68 6 0 0

4 7% starved 1 70 6 1 0

4 7% starved 1 72 5 0 0

4 7% starved 1 74 5 1 0

4 7% starved 1 78 4 0 0

4 7% starved 1 80 4 0 0

4 7% starved 1 82 4 0 0

4 7% starved 1 84 4 0 0

4 7% starved 1 86 4 1 0

4 7% starved 1 88 3 0 0

4 7% starved 1 90 3 0 0

4 7% starved 1 92 3 0 0

4 7% starved 1 94 3 0 0

4 7% starved 1 96 3 1 0

4 7% starved 1 100 2 1 0

4 7% starved 1 104 1 0 0

4 7% starved 1 106 1 0 0

4 7% starved 1 108 1 0 0

4 7% starved 1 110 1 0 0

4 7% starved 1 112 1 1 0

4 7% starved 1

4 7% starved 1

4 7% starved 1

4 7% starved 1

4 7% starved 1

4 7% starved 2 0 15 0 0

4 7% starved 2 12 15 0 0

4 7% starved 2 18 15 0 0

4 7% starved 2 24 15 0 0

4 7% starved 2 26 15 0 0

4 7% starved 2 28 15 0 0

4 7% starved 2 30 15 0 0

4 7% starved 2 32 15 0 0

4 7% starved 2 34 15 0 0

(8)

4 7% starved 2 36 15 0 0

4 7% starved 2 38 15 2 0

4 7% starved 2 40 13 0 0

4 7% starved 2 42 13 0 0

4 7% starved 2 43 13 0 0

4 7% starved 2 44 13 0 0

4 7% starved 2 46 13 1 0

4 7% starved 2 48 12 0 0

4 7% starved 2 50 12 0 0

4 7% starved 2 52 12 0 0

4 7% starved 2 54 12 0 0

4 7% starved 2 56 12 1 0

4 7% starved 2 58 11 0 0

4 7% starved 2 60 11 1 0

4 7% starved 2 62 10 0 0

4 7% starved 2 64 10 1 0

4 7% starved 2 66 9 0 0

4 7% starved 2 68 9 1 0

4 7% starved 2 70 8 0 0

4 7% starved 2 72 8 0 0

4 7% starved 2 74 8 0 0

4 7% starved 2 78 8 0 0

4 7% starved 2 80 8 1 0

4 7% starved 2 82 7 2 0

4 7% starved 2 84 5 1 0

4 7% starved 2 86 4 1 0

4 7% starved 2 88 3 1 0

4 7% starved 2 90 2 1 0

4 7% starved 2 92 1 0 0

4 7% starved 2 94 1 0 0

4 7% starved 2 96 1 0 0

4 7% starved 2 100 1 0 0

4 7% starved 2 104 1 0 0

4 7% starved 2 106 1 1 0

4 7% starved 2 108 0 0 0

4 7% starved 2 110 0 0 0

4 7% starved 2 112 0 0 0

4 7% starved 2

4 7% starved 2

4 7% starved 2

4 7% starved 2

4 7% starved 2

4 7% starved 3 0 17 0 0

4 7% starved 3 12 17 0 0

4 7% starved 3 18 17 0 0

4 7% starved 3 24 17 0 0

4 7% starved 3 26 17 0 0

4 7% starved 3 28 17 1 0

4 7% starved 3 30 16 0 0

4 7% starved 3 32 16 0 0

4 7% starved 3 34 16 0 0

4 7% starved 3 36 16 0 0

4 7% starved 3 38 16 0 0

4 7% starved 3 40 16 0 0

4 7% starved 3 42 16 0 0

(9)

4 7% starved 3 44 16 0 0

4 7% starved 3 46 16 0 0

4 7% starved 3 48 16 0 0

4 7% starved 3 50 16 0 0

4 7% starved 3 52 16 1 0

4 7% starved 3 54 15 0 0

4 7% starved 3 56 15 1 0

4 7% starved 3 58 14 1 0

4 7% starved 3 60 13 1 0

4 7% starved 3 62 12 0 0

4 7% starved 3 64 12 0 0

4 7% starved 3 66 12 1 0

4 7% starved 3 68 11 1 0

4 7% starved 3 70 10 1 0

4 7% starved 3 72 9 0 0

4 7% starved 3 74 9 1 0

4 7% starved 3 78 8 1 0

4 7% starved 3 80 7 0 0

4 7% starved 3 82 7 1 0

4 7% starved 3 84 6 1 0

4 7% starved 3 86 5 2 0

4 7% starved 3 88 3 0 0

4 7% starved 3 90 3 1 0

4 7% starved 3 92 2 0 0

4 7% starved 3 94 2 0 0

4 7% starved 3 96 2 1 0

4 7% starved 3 100 1 0 0

4 7% starved 3 104 1 0 0

4 7% starved 3 106 1 0 0

4 7% starved 3 108 1 0 0

4 7% starved 3 110 1 0 0

4 7% starved 3 112 1 0 1

4 7% starved 3

4 7% starved 3

4 7% starved 3

4 7% starved 3

4 7% starved 3

4 7% starved 4 0 17 0 0

4 7% starved 4 12 17 0 0

4 7% starved 4 18 17 0 0

4 7% starved 4 24 17 0 0

4 7% starved 4 26 17 0 0

4 7% starved 4 28 17 0 0

4 7% starved 4 30 17 0 0

4 7% starved 4 32 17 0 0

4 7% starved 4 34 17 0 0

4 7% starved 4 36 17 0 0

4 7% starved 4 38 17 0 0

4 7% starved 4 40 17 0 0

4 7% starved 4 42 17 0 0

4 7% starved 4 43 17 0 0

4 7% starved 4 44 17 0 0

4 7% starved 4 46 17 1 0

4 7% starved 4 48 16 1 0

4 7% starved 4 50 15 1 0

4 7% starved 4 52 14 0 0

(10)

4 7% starved 4 54 14 2 0

4 7% starved 4 56 12 2 0

4 7% starved 4 58 10 1 0

4 7% starved 4 60 9 0 0

4 7% starved 4 62 9 1 0

4 7% starved 4 64 8 1 0

4 7% starved 4 66 7 0 0

4 7% starved 4 68 7 2 0

4 7% starved 4 70 5 0 0

4 7% starved 4 72 5 1 0

4 7% starved 4 74 4 1 0

4 7% starved 4 78 3 0 0

4 7% starved 4 80 3 0 0

4 7% starved 4 82 3 1 0

4 7% starved 4 84 2 0 0

4 7% starved 4 86 2 1 0

4 7% starved 4 88 1 0 0

4 7% starved 4 90 1 0 0

4 7% starved 4 92 1 0 0

4 7% starved 4 94 1 0 0

4 7% starved 4 96 1 0 0

4 7% starved 4 100 1 0 0

4 7% starved 4 104 1 0 0

4 7% starved 4 106 1 0 0

4 7% starved 4 108 1 0 0

4 7% starved 4 110 1 0 0

4 7% starved 4 112 1 1 0

4 7% starved 4

4 7% starved 4

4 7% starved 4

4 7% starved 4

4 7% starved 4

4 7% starved 5 0 0 0 0

4 7% starved 5 12 0 0 0

4 7% starved 5 18 0 0 0

4 7% starved 5 24 0 0 0

4 7% starved 5 26 0 0 0

4 7% starved 5 28 0 0 0

4 7% starved 5 30 0 0 0

4 7% starved 5 32 0 0 0

4 7% starved 5 34 0 0 0

4 7% starved 5 36 0 0 0

4 7% starved 5 38 0 0 0

4 7% starved 5 40 0 0 0

4 7% starved 5 42 0 0 0

4 7% starved 5 43 0 0 0

4 7% starved 5 44 0 0 0

4 7% starved 5 46 0 0 0

4 7% starved 5 48 0 0 0

4 7% starved 5 50 0 0 0

4 7% starved 5 52 0 0 0

4 7% starved 5 54 0 0 0

4 7% starved 5 56 0 0 0

4 7% starved 5 58 0 0 0

4 7% starved 5 60 0 0 0

(11)

4 7% starved 5 64 0 0 0

4 7% starved 5 66 0 0 0

4 7% starved 5 68 0 0 0

4 7% starved 5 70 0 0 0

4 7% starved 5 72 0 0 0

4 7% starved 5 74 0 0 0

4 7% starved 5 78 0 0 0

4 7% starved 5 80 0 0 0

4 7% starved 5 82 0 0 0

4 7% starved 5 84 0 0 0

4 7% starved 5 86 0 0 0

4 7% starved 5 88 0 0 0

4 7% starved 5 90 0 0 0

4 7% starved 5 92 0 0 0

4 7% starved 5 94 0 0 0

4 7% starved 5 96 0 0 0

4 7% starved 5 100 0 0 0

4 7% starved 5 104 0 0 0

4 7% starved 5 106 0 0 0

4 7% starved 5 108 0 0 0

4 7% starved 5 110 0 0 0

4 7% starved 5 112 0 0 0

4 7% starved 5

4 7% starved 5

4 7% starved 5

4 7% starved 5

4 7% starved 5

4 11% control 1 0 18 0 0

4 11% control 1 12 18 0 0

4 11% control 1 18 18 0 0

4 11% control 1 24 18 2 0

4 11% control 1 26 16 0 0

4 11% control 1 28 16 0 0

4 11% control 1 30 16 0 0

4 11% control 1 32 16 0 0

4 11% control 1 34 16 0 0

4 11% control 1 36 16 0 0

4 11% control 1 38 16 0 0

4 11% control 1 40 16 0 0

4 11% control 1 42 16 0 0

4 11% control 1 43 16 0 0

4 11% control 1 44 16 0 0

4 11% control 1 46 16 0 0

4 11% control 1 48 16 0 0

4 11% control 1 50 16 0 0

4 11% control 1 52 16 0 0

4 11% control 1 54 16 0 0

4 11% control 1 56 16 0 0

4 11% control 1 58 16 0 0

4 11% control 1 60 16 0 0

4 11% control 1 62 16 0 0

4 11% control 1 64 16 0 0

4 11% control 1 66 16 0 0

4 11% control 1 68 16 0 0

4 11% control 1 70 16 0 0

4 11% control 1 72 16 0 0

(12)

4 11% control 1 74 16 0 0

4 11% control 1 78 16 0 0

4 11% control 1 80 16 0 0

4 11% control 1 82 16 0 0

4 11% control 1 84 16 0 0

4 11% control 1 86 16 0 0

4 11% control 1 88 16 0 0

4 11% control 1 90 16 0 0

4 11% control 1 92 16 0 0

4 11% control 1 94 16 0 0

4 11% control 1 96 16 0 0

4 11% control 1 100 16 0 0

4 11% control 1 104 16 0 0

4 11% control 1 106 16 0 16

4 11% control 1 108 0 0 0

4 11% control 1 110 0 0 0

4 11% control 1 112 0 0 0

4 11% control 1

4 11% control 1

4 11% control 1

4 11% control 1

4 11% control 1

4 11% starved 1 0 18 0 0

4 11% starved 1 12 18 0 0

4 11% starved 1 18 18 0 0

4 11% starved 1 24 18 1 0

4 11% starved 1 26 17 0 0

4 11% starved 1 28 17 0 0

4 11% starved 1 30 17 2 0

4 11% starved 1 32 15 0 0

4 11% starved 1 34 15 0 0

4 11% starved 1 36 15 2 0

4 11% starved 1 38 13 0 0

4 11% starved 1 40 13 2 0

4 11% starved 1 42 11 1 0

4 11% starved 1 43 10 0 0

4 11% starved 1 44 10 1 0

4 11% starved 1 46 9 0 0

4 11% starved 1 48 9 1 0

4 11% starved 1 50 8 1 0

4 11% starved 1 52 7 0 0

4 11% starved 1 54 7 0 0

4 11% starved 1 56 7 0 0

4 11% starved 1 58 7 0 0

4 11% starved 1 60 7 1 0

4 11% starved 1 62 6 0 0

4 11% starved 1 64 6 1 0

4 11% starved 1 66 5 0 0

4 11% starved 1 68 5 2 0

4 11% starved 1 70 3 0 0

4 11% starved 1 72 3 0 0

4 11% starved 1 74 3 0 0

4 11% starved 1 78 3 0 0

4 11% starved 1 80 3 1 0

4 11% starved 1 82 2 0 0

(13)

4 11% starved 1 86 2 0 0

4 11% starved 1 88 2 0 0

4 11% starved 1 90 2 1 0

4 11% starved 1 92 1 0 0

4 11% starved 1 94 1 1 0

4 11% starved 1 96 0 0 0

4 11% starved 1 100 0 0 0

4 11% starved 1 104 0 0 0

4 11% starved 1 106 0 0 0

4 11% starved 1 108 0 0 0

4 11% starved 1 110 0 0 0

4 11% starved 1 112 0 0 0

4 11% starved 1

4 11% starved 1

4 11% starved 1

4 11% starved 1

4 11% starved 1

4 11% starved 2 0 16 0 0

4 11% starved 2 12 16 0 0

4 11% starved 2 18 16 0 0

4 11% starved 2 24 16 0 0

4 11% starved 2 26 16 1 0

4 11% starved 2 28 15 1 0

4 11% starved 2 30 14 1 0

4 11% starved 2 32 13 0 0

4 11% starved 2 34 13 2 0

4 11% starved 2 36 11 0 0

4 11% starved 2 38 11 2 0

4 11% starved 2 40 9 0 0

4 11% starved 2 42 9 1 0

4 11% starved 2 43 8 0 0

4 11% starved 2 44 8 2 0

4 11% starved 2 46 6 0 0

4 11% starved 2 48 6 1 0

4 11% starved 2 50 5 0 0

4 11% starved 2 52 5 1 0

4 11% starved 2 54 4 0 0

4 11% starved 2 56 4 0 0

4 11% starved 2 58 4 1 0

4 11% starved 2 60 3 1 0

4 11% starved 2 62 2 1 0

4 11% starved 2 64 1 0 0

4 11% starved 2 66 1 0 0

4 11% starved 2 68 1 0 0

4 11% starved 2 70 1 0 0

4 11% starved 2 72 1 0 0

4 11% starved 2 74 1 0 0

4 11% starved 2 78 1 1 0

4 11% starved 2 80 0 0 0

4 11% starved 2 82 0 0 0

4 11% starved 2 84 0 0 0

4 11% starved 2 86 0 0 0

4 11% starved 2 88 0 0 0

4 11% starved 2 90 0 0 0

4 11% starved 2 92 0 0 0

4 11% starved 2 94 0 0 0

(14)

4 11% starved 2 96 0 0 0

4 11% starved 2 100 0 0 0

4 11% starved 2 104 0 0 0

4 11% starved 2 106 0 0 0

4 11% starved 2 108 0 0 0

4 11% starved 2 110 0 0 0

4 11% starved 2 112 0 0 0

4 11% starved 2

4 11% starved 2

4 11% starved 2

4 11% starved 2

4 11% starved 2

4 11% starved 3 0 16 0 0

4 11% starved 3 12 16 1 0

4 11% starved 3 18 15 0 0

4 11% starved 3 24 15 0 0

4 11% starved 3 26 15 0 0

4 11% starved 3 28 15 1 0

4 11% starved 3 30 14 0 0

4 11% starved 3 32 14 0 0

4 11% starved 3 34 14 1 0

4 11% starved 3 36 13 2 0

4 11% starved 3 38 11 0 0

4 11% starved 3 40 11 1 0

4 11% starved 3 42 10 1 0

4 11% starved 3 43 9 0 0

4 11% starved 3 44 9 0 0

4 11% starved 3 46 9 0 0

4 11% starved 3 48 9 0 0

4 11% starved 3 50 9 0 0

4 11% starved 3 52 9 1 0

4 11% starved 3 54 8 0 0

4 11% starved 3 56 8 0 0

4 11% starved 3 58 8 0 0

4 11% starved 3 60 8 1 0

4 11% starved 3 62 7 0 0

4 11% starved 3 64 7 0 0

4 11% starved 3 66 7 2 0

4 11% starved 3 68 5 0 0

4 11% starved 3 70 5 1 0

4 11% starved 3 72 4 0 0

4 11% starved 3 74 4 1 0

4 11% starved 3 78 3 0 0

4 11% starved 3 80 3 0 0

4 11% starved 3 82 3 1 0

4 11% starved 3 84 2 1 0

4 11% starved 3 86 1 0 0

4 11% starved 3 88 1 0 0

4 11% starved 3 90 1 0 0

4 11% starved 3 92 1 0 0

4 11% starved 3 94 1 0 0

4 11% starved 3 96 1 1 0

4 11% starved 3 100 0 0 0

4 11% starved 3 104 0 0 0

4 11% starved 3 106 0 0 0

(15)

4 11% starved 3 110 0 0 0

4 11% starved 3 112 0 0 0

4 11% starved 3

4 11% starved 3

4 11% starved 3

4 11% starved 3

4 11% starved 3

4 11% starved 4 0 16 0 0

4 11% starved 4 12 16 0 0

4 11% starved 4 18 16 0 0

4 11% starved 4 24 16 1 0

4 11% starved 4 26 15 1 0

4 11% starved 4 28 14 2 0

4 11% starved 4 30 12 2 0

4 11% starved 4 32 10 2 0

4 11% starved 4 34 8 0 0

4 11% starved 4 36 8 0 0

4 11% starved 4 38 8 1 0

4 11% starved 4 40 7 0 0

4 11% starved 4 42 7 2 0

4 11% starved 4 43 5 0 0

4 11% starved 4 44 5 0 0

4 11% starved 4 46 5 0 0

4 11% starved 4 48 5 0 0

4 11% starved 4 50 5 0 0

4 11% starved 4 52 5 0 0

4 11% starved 4 54 5 0 0

4 11% starved 4 56 5 0 0

4 11% starved 4 58 5 1 0

4 11% starved 4 60 4 1 0

4 11% starved 4 62 3 0 0

4 11% starved 4 64 3 0 0

4 11% starved 4 66 3 0 0

4 11% starved 4 68 3 0 0

4 11% starved 4 70 3 2 0

4 11% starved 4 72 1 0 0

4 11% starved 4 74 1 0 0

4 11% starved 4 78 1 0 0

4 11% starved 4 80 1 0 0

4 11% starved 4 82 1 1 0

4 11% starved 4 84 0 0 0

4 11% starved 4 86 0 0 0

4 11% starved 4 88 0 0 0

4 11% starved 4 90 0 0 0

4 11% starved 4 92 0 0 0

4 11% starved 4 94 0 0 0

4 11% starved 4 96 0 0 0

4 11% starved 4 100 0 0 0

4 11% starved 4 104 0 0 0

4 11% starved 4 106 0 0 0

4 11% starved 4 108 0 0 0

4 11% starved 4 110 0 0 0

4 11% starved 4 112 0 0 0

4 11% starved 4

4 11% starved 4

4 11% starved 4

(16)

4 11% starved 4

4 11% starved 4

4 11% starved 5 0 0 0 0

4 11% starved 5 12 0 0 0

4 11% starved 5 18 0 0 0

4 11% starved 5 24 0 0 0

4 11% starved 5 26 0 0 0

4 11% starved 5 28 0 0 0

4 11% starved 5 30 0 0 0

4 11% starved 5 32 0 0 0

4 11% starved 5 34 0 0 0

4 11% starved 5 36 0 0 0

4 11% starved 5 38 0 0 0

4 11% starved 5 40 0 0 0

4 11% starved 5 42 0 0 0

4 11% starved 5 43 0 0 0

4 11% starved 5 44 0 0 0

4 11% starved 5 46 0 0 0

4 11% starved 5 48 0 0 0

4 11% starved 5 50 0 0 0

4 11% starved 5 52 0 0 0

4 11% starved 5 54 0 0 0

4 11% starved 5 56 0 0 0

4 11% starved 5 58 0 0 0

4 11% starved 5 60 0 0 0

4 11% starved 5 62 0 0 0

4 11% starved 5 64 0 0 0

4 11% starved 5 66 0 0 0

4 11% starved 5 68 0 0 0

4 11% starved 5 70 0 0 0

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4 11% starved 5 86 0 0 0

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4 11% starved 5 100 0 0 0

4 11% starved 5 104 0 0 0

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4 11% starved 5 108 0 0 0

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4 11% starved 5 112 0 0 0

4 11% starved 5

4 11% starved 5

4 11% starved 5

4 11% starved 5

4 11% starved 5

4 16% control 1 0 16 0 0

4 16% control 1 12 16 0 0

(17)

4 16% control 1 24 16 0 0

4 16% control 1 26 16 0 0

4 16% control 1 28 16 0 0

4 16% control 1 30 16 0 0

4 16% control 1 32 16 0 0

4 16% control 1 34 16 0 0

4 16% control 1 36 16 0 0

4 16% control 1 38 16 0 0

4 16% control 1 40 16 0 0

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4 16% control 1 44 16 0 0

4 16% control 1 46 16 0 0

4 16% control 1 48 16 0 0

4 16% control 1 50 16 0 0

4 16% control 1 52 16 0 0

4 16% control 1 54 16 0 0

4 16% control 1 56 16 0 0

4 16% control 1 58 16 0 0

4 16% control 1 60 16 0 0

4 16% control 1 62 16 0 0

4 16% control 1 64 16 0 0

4 16% control 1 66 16 0 0

4 16% control 1 68 16 0 0

4 16% control 1 70 16 0 0

4 16% control 1 72 16 0 0

4 16% control 1 74 16 0 0

4 16% control 1 78 16 0 0

4 16% control 1 80 16 0 0

4 16% control 1 82 16 0 0

4 16% control 1 84 16 0 0

4 16% control 1 86 16 0 0

4 16% control 1 88 16 0 0

4 16% control 1 90 16 0 0

4 16% control 1 92 16 0 0

4 16% control 1 94 16 0 0

4 16% control 1 96 16 0 0

4 16% control 1 100 16 0 0

4 16% control 1 104 16 0 0

4 16% control 1 106 16 0 16

4 16% control 1 108 0 0 0

4 16% control 1 110 0 0 0

4 16% control 1 112 0 0 0

4 16% control 1

4 16% control 1

4 16% control 1

4 16% control 1

4 16% control 1

4 16% starved 1 0 17 0 0

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4 16% starved 1 18 17 0 0

4 16% starved 1 24 17 0 0

4 16% starved 1 26 17 1 0

4 16% starved 1 28 16 1 0

4 16% starved 1 30 15 0 0

4 16% starved 1 32 15 1 0

(18)

4 16% starved 1 34 14 2 0

4 16% starved 1 36 12 1 0

4 16% starved 1 38 11 0 0

4 16% starved 1 40 11 2 0

4 16% starved 1 42 9 0 0

4 16% starved 1 43 9 0 0

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4 16% starved 1

4 16% starved 1

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4 16% starved 2 34 15 0 0

4 16% starved 2 36 15 3 0

4 16% starved 2 38 12 0 0

4 16% starved 2 40 12 1 0

(19)

4 16% starved 2 43 11 0 0

4 16% starved 2 44 11 0 0

4 16% starved 2 46 11 0 0

4 16% starved 2 48 11 0 0

4 16% starved 2 50 11 1 0

4 16% starved 2 52 10 0 0

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4 16% starved 2 100 0 0 0

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4 16% starved 2 108 0 0 0

4 16% starved 2 110 0 0 0

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4 16% starved 2

4 16% starved 2

4 16% starved 2

4 16% starved 2

4 16% starved 2

4 16% starved 3 0 18 0 0

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4 16% starved 3 18 18 0 0

4 16% starved 3 24 18 2 0

4 16% starved 3 26 16 0 0

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4 16% starved 3 30 16 1 0

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4 16% starved 3 34 15 0 0

4 16% starved 3 36 15 1 0

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4 16% starved 3 43 13 0 0

4 16% starved 3 44 13 0 0

4 16% starved 3 46 13 3 0

4 16% starved 3 48 10 0 0

4 16% starved 3 50 10 4 0

(20)

4 16% starved 3 52 6 1 0

4 16% starved 3 54 5 0 0

4 16% starved 3 56 5 0 0

4 16% starved 3 58 5 1 0

4 16% starved 3 60 4 0 0

4 16% starved 3 62 4 0 0

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4 16% starved 3 70 4 0 0

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4 16% starved 3 78 4 2 0

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4 16% starved 3 84 1 0 0

4 16% starved 3 86 1 0 0

4 16% starved 3 88 1 0 0

4 16% starved 3 90 1 0 0

4 16% starved 3 92 1 0 0

4 16% starved 3 94 1 0 0

4 16% starved 3 96 1 0 0

4 16% starved 3 100 1 0 0

4 16% starved 3 104 1 0 0

4 16% starved 3 106 1 1 0

4 16% starved 3 108 0 0 0

4 16% starved 3 110 0 0 0

4 16% starved 3 112 0 0 0

4 16% starved 3

4 16% starved 3

4 16% starved 3

4 16% starved 3

4 16% starved 3

4 16% starved 4 0 15 0 0

4 16% starved 4 12 15 0 0

4 16% starved 4 18 15 0 0

4 16% starved 4 24 15 0 0

4 16% starved 4 26 15 1 0

4 16% starved 4 28 14 0 0

4 16% starved 4 30 14 0 0

4 16% starved 4 32 14 0 0

4 16% starved 4 34 14 1 0

4 16% starved 4 36 13 3 0

4 16% starved 4 38 10 1 0

4 16% starved 4 40 9 2 0

4 16% starved 4 42 7 1 0

4 16% starved 4 43 6 1 0

4 16% starved 4 44 5 0 0

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4 16% starved 4 48 4 0 0

4 16% starved 4 50 4 1 0

4 16% starved 4 52 3 0 0

4 16% starved 4 54 3 0 0

4 16% starved 4 56 3 0 0

4 16% starved 4 58 3 1 0

(21)

4 16% starved 4 62 2 0 0

4 16% starved 4 64 2 0 0

4 16% starved 4 66 2 0 0

4 16% starved 4 68 2 0 0

4 16% starved 4 70 2 0 0

4 16% starved 4 72 2 1 0

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4 16% starved 4 90 1 1 0

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4 16% starved 4 96 0 0 0

4 16% starved 4 100 0 0 0

4 16% starved 4 104 0 0 0

4 16% starved 4 106 0 0 0

4 16% starved 4 108 0 0 0

4 16% starved 4 110 0 0 0

4 16% starved 4 112 0 0 0

4 16% starved 4

4 16% starved 4

4 16% starved 4

4 16% starved 4

4 16% starved 4

4 16% starved 5 0 0 0 0

4 16% starved 5 12 0 0 0

4 16% starved 5 18 0 0 0

4 16% starved 5 24 0 0 0

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4 16% starved 5 34 0 0 0

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4 16% starved 5 68 0 0 0

4 16% starved 5 70 0 0 0

(22)

4 16% starved 5 72 0 0 0

4 16% starved 5 74 0 0 0

4 16% starved 5 78 0 0 0

4 16% starved 5 80 0 0 0

4 16% starved 5 82 0 0 0

4 16% starved 5 84 0 0 0

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4 16% starved 5 100 0 0 0

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4 16% starved 5 106 0 0 0

4 16% starved 5 108 0 0 0

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4 16% starved 5

4 16% starved 5

4 16% starved 5

4 16% starved 5

4 16% starved 5

20 7% control 1 0 14 0 0

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20 7% control 1 11 14 0 0

20 7% control 1 13 14 0 0

20 7% control 1 14 14 0 0

20 7% control 1 16 14 0 0

20 7% control 1 18 14 0 0

20 7% control 1 20 14 0 0

20 7% control 1 22 14 0 0

20 7% control 1 24 14 0 0

20 7% control 1 26 14 0 0

20 7% control 1 28 14 0 0

20 7% control 1 30 14 0 0

20 7% control 1 32 14 0 0

20 7% control 1 34 14 0 0

20 7% control 1 36 14 0 0

20 7% control 1 38 14 0 0

20 7% control 1 40 14 0 0

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20 7% control 1 48 14 0 0

20 7% control 1 50 14 0 0

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20 7% control 1 54 14 0 0

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20 7% control 1 60 14 0 0

20 7% control 1 62 14 0 0

20 7% control 1 64 14 0 0

20 7% control 1 66 14 0 0

20 7% control 1 68 14 0 0

20 7% control 1 72 14 0 0

(23)

20 7% control 1 78 14 0 0

20 7% control 1 80 14 0 0

20 7% control 1 82 14 0 0

20 7% control 1 84 14 0 0

20 7% control 1 86 14 0 0

20 7% control 1 88 14 0 0

20 7% control 1 90 14 0 0

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20 7% control 1 98 14 0 0

20 7% control 1 100 14 0 14

20 7% control 1 102 0 0 0

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20 7% control 1

20 7% control 1

20 7% control 1

20 7% control 1

20 7% control 1

20 7% control 1

20 7% starved 1 0 15 0 0

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20 7% starved 1 30 11 0 0

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20 7% starved 1 34 10 2 0

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20 7% starved 1 82 1 1 0

20 7% starved 1 84 0 0 0

20 7% starved 1 86 0 0 0

(24)

20 7% starved 1 88 0 0 0

20 7% starved 1 90 0 0 0

20 7% starved 1 94 0 0 0

20 7% starved 1 98 0 0 0

20 7% starved 1 100 0 0 0

20 7% starved 1 102 0 0 0

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20 7% starved 1

20 7% starved 1

20 7% starved 1

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20 7% starved 1

20 7% starved 1

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20 7% starved 2 18 16 0 0

20 7% starved 2 20 16 0 0

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20 7% starved 2 24 16 0 0

20 7% starved 2 26 16 0 0

20 7% starved 2 28 16 0 0

20 7% starved 2 30 16 3 0

20 7% starved 2 32 13 1 0

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20 7% starved 2 36 9 0 0

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20 7% starved 2 72 2 0 0

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20 7% starved 2 80 2 0 0

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20 7% starved 2 86 1 0 0

20 7% starved 2 88 1 0 0

20 7% starved 2 90 1 1 0

20 7% starved 2 94 0 0 0

20 7% starved 2 98 0 0 0

(25)

20 7% starved 2 102 0 0 0

20 7% starved 2 106 0 0 0

20 7% starved 2

20 7% starved 2

20 7% starved 2

20 7% starved 2

20 7% starved 2

20 7% starved 2

20 7% starved 3 0 15 0 0

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20 7% starved 3 36 9 1 0

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20 7% starved 3 84 0 0 0

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20 7% starved 3 88 0 0 0

20 7% starved 3 90 0 0 0

20 7% starved 3 94 0 0 0

20 7% starved 3 98 0 0 0

20 7% starved 3 100 0 0 0

20 7% starved 3 102 0 0 0

20 7% starved 3 106 0 0 0

20 7% starved 3

20 7% starved 3

20 7% starved 3

(26)

20 7% starved 3

20 7% starved 3

20 7% starved 3

20 7% starved 4 0 15 0 0

20 7% starved 4 8 15 0 0

20 7% starved 4 11 15 0 0

20 7% starved 4 13 15 0 0

20 7% starved 4 14 15 0 0

20 7% starved 4 16 15 0 0

20 7% starved 4 18 15 0 0

20 7% starved 4 20 15 0 0

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20 7% starved 4 26 14 0 0

20 7% starved 4 28 14 0 0

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20 7% starved 4 32 12 1 0

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20 7% starved 4 46 6 3 0

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20 7% starved 4 68 2 0 0

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20 7% starved 4 90 1 0 0

20 7% starved 4 94 1 1 0

20 7% starved 4 98 0 0 0

20 7% starved 4 100 0 0 0

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20 7% starved 4 106 0 0 0

20 7% starved 4

20 7% starved 4

20 7% starved 4

20 7% starved 4

20 7% starved 4

20 7% starved 4

20 7% starved 5 0 19 0 0

(27)

20 7% starved 5 11 19 0 0

20 7% starved 5 13 19 0 0

20 7% starved 5 14 19 0 0

20 7% starved 5 16 19 0 0

20 7% starved 5 18 19 0 0

20 7% starved 5 20 19 0 0

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20 7% starved 5 28 19 1 0

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20 7% starved 5 100 1 0 0

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20 7% starved 5

20 7% starved 5

20 7% starved 5

20 7% starved 5

20 7% starved 5

20 7% starved 5

20 11% control 1 0 16 0 0

20 11% control 1 8 16 0 0

20 11% control 1 11 16 0 0

20 11% control 1 13 16 0 0

20 11% control 1 14 16 0 0

20 11% control 1 16 16 0 0

20 11% control 1 18 16 0 0

(28)

20 11% control 1 20 16 0 0

20 11% control 1 22 16 0 0

20 11% control 1 24 16 0 0

20 11% control 1 26 16 0 0

20 11% control 1 28 16 0 0

20 11% control 1 30 16 0 0

20 11% control 1 32 16 0 0

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20 11% control 1 50 16 0 0

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20 11% control 1 54 16 0 0

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20 11% control 1 60 16 0 0

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20 11% control 1 72 16 0 0

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20 11% control 1 78 16 0 0

20 11% control 1 80 16 0 0

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20 11% control 1 84 16 0 0

20 11% control 1 86 16 0 0

20 11% control 1 88 16 0 0

20 11% control 1 90 16 0 0

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20 11% control 1 98 16 0 0

20 11% control 1 100 16 0 16

20 11% control 1 102 0 0 0

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20 11% control 1

20 11% control 1

20 11% control 1

20 11% control 1

20 11% control 1

20 11% control 1

20 11% starved 1 0 15 0 0

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20 11% starved 1 18 15 0 0

20 11% starved 1 20 15 0 0

20 11% starved 1 22 15 0 0

20 11% starved 1 24 15 1 0

20 11% starved 1 26 14 0 0

(29)

20 11% starved 1 30 13 0 0

20 11% starved 1 32 13 1 0

20 11% starved 1 34 12 1 0

20 11% starved 1 36 11 1 0

20 11% starved 1 38 10 2 0

20 11% starved 1 40 8 1 0

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20 11% starved 1 50 1 1 0

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20 11% starved 1

20 11% starved 1

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20 11% starved 2 38 11 1 0

(30)

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20 11% starved 2

20 11% starved 2

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(31)

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20 11% starved 3

20 11% starved 3

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(32)

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(33)

20 11% starved 5 76 2 1 0

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20 11% starved 5

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20 16% control 1 0 15 0 0

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20 16% control 1 84 15 0 0

(34)

20 16% control 1 86 15 0 0

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(35)

20 16% starved 1 100 1 0 0

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20 16% starved 1

20 16% starved 1

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20 16% starved 2

20 16% starved 2

(36)

20 16% starved 2

20 16% starved 2

20 16% starved 2

20 16% starved 2

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20 16% starved 3

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20 16% starved 3

20 16% starved 3

(37)

20 16% starved 4 8 13 0 0

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20 16% starved 4

20 16% starved 4

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(38)

20 16% starved 5 18 14 0 0

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33 7% control 1 0 14 0 0

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(39)

33 7% control 1 30 14 0 0

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33 7% control 1

33 7% control 1

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(40)

33 7% starved 1 40 5 0 0

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33 7% starved 2 46 2 1 0

(41)

33 7% starved 2 50 1 1 0

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33 7% starved 2

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33 7% starved 3 56 1 0 0

33 7% starved 3 58 1 0 0

(42)

33 7% starved 3 60 1 0 0

33 7% starved 3 62 1 0 0

33 7% starved 3 64 1 0 0

33 7% starved 3 66 1 0 0

33 7% starved 3 68 1 0 0

33 7% starved 3 70 1 0 0

33 7% starved 3 72 1 0 0

33 7% starved 3 74 1 1 0

33 7% starved 3 76 0 0 0

33 7% starved 3 78 0 0 0

33 7% starved 3 80 0 0 0

33 7% starved 3 82 0 0 0

33 7% starved 3 84 0 0 0

33 7% starved 3 86 0 0 0

33 7% starved 3 88 0 0 0

33 7% starved 3 90 0 0 0

33 7% starved 3 92 0 0 0

33 7% starved 3 94 0 0 0

33 7% starved 3 96 0 0 0

33 7% starved 3 98 0 0 0

33 7% starved 3 112 0 0 0

33 7% starved 3

33 7% starved 3

33 7% starved 3

33 7% starved 3

33 7% starved 4 0 18 0 0

33 7% starved 4 10 18 0 0

33 7% starved 4 12 18 0 0

33 7% starved 4 14 18 0 0

33 7% starved 4 16 18 0 0

33 7% starved 4 18 18 1 0

33 7% starved 4 20 17 0 0

33 7% starved 4 22 17 0 0

33 7% starved 4 24 17 1 0

33 7% starved 4 26 16 0 0

33 7% starved 4 28 16 2 0

33 7% starved 4 30 14 0 0

33 7% starved 4 32 14 1 0

33 7% starved 4 34 13 2 0

33 7% starved 4 36 11 1 0

33 7% starved 4 38 10 1 0

33 7% starved 4 40 9 1 0

33 7% starved 4 42 8 0 0

33 7% starved 4 44 8 1 0

33 7% starved 4 46 7 2 0

33 7% starved 4 48 5 2 0

33 7% starved 4 50 3 0 0

33 7% starved 4 52 3 0 0

33 7% starved 4 54 3 1 0

33 7% starved 4 56 2 0 0

33 7% starved 4 58 2 1 0

33 7% starved 4 60 1 0 0

33 7% starved 4 62 1 0 0

33 7% starved 4 64 1 0 0

33 7% starved 4 66 1 1 0

(43)

33 7% starved 4 70 0 0 0

33 7% starved 4 72 0 0 0

33 7% starved 4 74 0 0 0

33 7% starved 4 76 0 0 0

33 7% starved 4 78 0 0 0

33 7% starved 4 80 0 0 0

33 7% starved 4 82 0 0 0

33 7% starved 4 84 0 0 0

33 7% starved 4 86 0 0 0

33 7% starved 4 88 0 0 0

33 7% starved 4 90 0 0 0

33 7% starved 4 92 0 0 0

33 7% starved 4 94 0 0 0

33 7% starved 4 96 0 0 0

33 7% starved 4 98 0 0 0

33 7% starved 4 112 0 0 0

33 7% starved 4

33 7% starved 4

33 7% starved 4

33 7% starved 4

33 7% starved 5 0 15 0 0

33 7% starved 5 10 15 0 0

33 7% starved 5 12 15 0 0

33 7% starved 5 14 15 0 0

33 7% starved 5 16 15 0 0

33 7% starved 5 18 15 1 0

33 7% starved 5 20 14 0 0

33 7% starved 5 22 14 2 0

33 7% starved 5 24 12 1 0

33 7% starved 5 26 11 0 0

33 7% starved 5 28 11 0 0

33 7% starved 5 30 11 0 0

33 7% starved 5 32 11 0 0

33 7% starved 5 34 11 1 0

33 7% starved 5 36 10 2 0

33 7% starved 5 38 8 2 0

33 7% starved 5 40 6 0 0

33 7% starved 5 42 6 1 0

33 7% starved 5 44 5 2 0

33 7% starved 5 46 3 2 0

33 7% starved 5 48 1 0 0

33 7% starved 5 50 1 0 0

33 7% starved 5 52 1 0 0

33 7% starved 5 54 1 0 0

33 7% starved 5 56 1 1 0

33 7% starved 5 58 0 0 0

33 7% starved 5 60 0 0 0

33 7% starved 5 62 0 0 0

33 7% starved 5 64 0 0 0

33 7% starved 5 66 0 0 0

33 7% starved 5 68 0 0 0

33 7% starved 5 70 0 0 0

33 7% starved 5 72 0 0 0

33 7% starved 5 74 0 0 0

33 7% starved 5 76 0 0 0

33 7% starved 5 78 0 0 0

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