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fig|349741.6.peg.1170 [Akkermansia muciniphila ATCC BAA-835] fig|349741.6.peg.1214 [Akkermansia muciniphila ATCC BAA-835] 1.000

fig|537012.5.peg.1177 [Bacteroides cellulosilyticus DSM 14838]

fig|537012.5.peg.2148 [Bacteroides cellulosilyticus DSM 14838] fig|469592.4.peg.783 [Bacteroides sp. 3_1_19]

fig|563193.3.peg.708 [Parabacteroides sp. D13] fig|469589.3.peg.1302 [Bacteroides sp. 2_1_33B]

fig|435591.13.peg.3875 [Parabacteroides distasonis ATCC 8503] fig|658661.3.peg.2636 [Parabacteroides sp. D25]

fig|457388.5.peg.31 [Bacteroides sp. 2_1_7] 0.837

0.883 1.000

fig|471870.8.peg.170 [Bacteroides intestinalis DSM 17393]

fig|483216.6.peg.222 [Bacteroides eggerthii DSM 20697] 0.943

fig|226186.12.peg.4737 [Bacteroides thetaiotaomicron VPI-5482] fig|665953.3.peg.2037 [Bacteroides eggerthii 1_2_48FAA]

fig|483216.6.peg.57 [Bacteroides eggerthii DSM 20697] 0.254

1.000

fig|888832.3.peg.1915 [Prevotella salivae DSM 15606] fig|411901.7.peg.3360 [Bacteroides caccae ATCC 43185]

fig|411477.4.peg.3080 [Parabacteroides merdae ATCC 43184] fig|537006.5.peg.2688 [Parabacteroides johnsonii DSM 18315]

fig|435591.13.peg.357 [Parabacteroides distasonis ATCC 8503] fig|658661.3.peg.3816 [Parabacteroides sp. D25] fig|457388.5.peg.3577 [Bacteroides sp. 2_1_7] fig|469592.4.peg.3643 [Bacteroides sp. 3_1_19] 0.142

0.975

fig|435591.13.peg.2062 [Parabacteroides distasonis ATCC 8503] fig|563193.3.peg.3088 [Parabacteroides sp. D13]

fig|469589.3.peg.2654 [Bacteroides sp. 2_1_33B] fig|763034.3.peg.2706 [Bacteroides fluxus YIT 12057]

fig|702446.3.peg.43 [Bacteroides vulgatus PC510] fig|483217.6.peg.1515 [Bacteroides dorei DSM 17855] fig|457395.6.peg.3396 [Bacteroides sp. 9_1_42FAA] fig|457391.3.peg.1795 [Bacteroides sp. 3_1_33FAA] 0.702

fig|457394.3.peg.479 [Bacteroides sp. 4_3_47FAA] fig|469593.3.peg.760 [Bacteroides sp. 3_1_40A] fig|435590.9.peg.1317 [Bacteroides vulgatus ATCC 8482] 0.973

0.998 0.979

0.093 0.931

fig|547042.5.peg.2915 [Bacteroides coprophilus DSM 18228] fig|449673.7.peg.2244 [Bacteroides stercoris ATCC 43183]

fig|457391.3.peg.3740 [Bacteroides sp. 3_1_33FAA] fig|457394.3.peg.2785 [Bacteroides sp. 4_3_47FAA] fig|471870.8.peg.790 [Bacteroides intestinalis DSM 17393]

fig|537012.5.peg.1189 [Bacteroides cellulosilyticus DSM 14838] 0.688

fig|457395.6.peg.3996 [Bacteroides sp. 9_1_42FAA] fig|483217.6.peg.1086 [Bacteroides dorei DSM 17855] fig|469593.3.peg.400 [Bacteroides sp. 3_1_40A]

fig|457394.3.peg.1711 [Bacteroides sp. 4_3_47FAA] fig|702446.3.peg.710 [Bacteroides vulgatus PC510] fig|435590.9.peg.553 [Bacteroides vulgatus ATCC 8482]

fig|457391.3.peg.3674 [Bacteroides sp. 3_1_33FAA]

fig|226186.12.peg.3239 [Bacteroides thetaiotaomicron VPI-5482] fig|457424.5.peg.3293 [Bacteroides fragilis 3_1_12]

fig|484018.6.peg.894 [Bacteroides plebeius DSM 17135] fig|226186.12.peg.1666 [Bacteroides thetaiotaomicron VPI-5482] fig|469586.3.peg.4806 [Bacteroides sp. 1_1_6]

fig|469585.3.peg.3199 [Bacteroides sp. 1_1_14]

fig|483215.6.peg.3070 [Bacteroides finegoldii DSM 17565] fig|556258.5.peg.4449 [Bacteroides sp. D1]

fig|585544.3.peg.3482 [Bacteroides sp. D22] fig|469588.3.peg.4552 [Bacteroides sp. 2_1_22]

fig|702444.3.peg.3890 [Bacteroides ovatus SD CC 2a] fig|657309.4.peg.3619 [Bacteroides xylanisolvens XB1A] fig|457387.3.peg.5182 [Bacteroides sp. 1_1_30] fig|702447.3.peg.2099 [Bacteroides xylanisolvens SD CC 1b] fig|702443.3.peg.4195 [Bacteroides ovatus SD CMC 3f]

fig|411901.7.peg.2666 [Bacteroides caccae ATCC 43185] fig|457390.3.peg.1590 [Bacteroides sp. 3_1_23] fig|556259.3.peg.3264 [Bacteroides sp. D2] fig|411476.11.peg.3719 [Bacteroides ovatus ATCC 8483]

fig|469590.5.peg.150 [Bacteroides sp. 2_2_4] 0.960 0.490 0.883 0.901 0.219 0.988 0.948 0.969 0.848 0.996 0.985 0.320 0.253 0.998

fig|702444.3.peg.3489 [Bacteroides ovatus SD CC 2a]

0.1

Figure S8. Maximum-likelihood tree for the Gns (N-acetyl-D-glucosamine specific mucin-desulfating sulfatase) proteins. Predicted sulfatase from Bacteroides ovatus SD

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