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Observation of extensive chromosome axis remodeling during the "Diffuse-Phase" of meiosis in large genome cereals

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Academic year: 2021

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FIGURE 1 | Super resolution microscopy of synapsis in large genome cereals. (i) Cartoon of cycle of chromatin expansion and contraction as described in Kleckner et al
FIGURE 2 | Modeling of the tinsel structure in wt and des10. Modeling the tinsel structure in wt (a) and des10 (b) by creating “surfaces” for ASY1 and ZYP1 using Imaris surface tool (a,b)
FIGURE 3 | ASY1 re-organization during desynapsis. De-synapsis was monitored with 3D-SIM using ASY1 (green) and ZYP1 (magenta) in barley wild type (a–f) and des10 (g–l)
FIGURE 4 | ASY1 re-organization during desynapsis and chromosome segregation. Strong ASY1 signal are detected after prophase I during chromosome segregation in both wild type (a–f) and des10 (g–m)

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