• Aucun résultat trouvé

Studying transmission activation of aphid-vectored Cauliflower mosaic virus

N/A
N/A
Info
Télécharger
Protected

Academic year: 2021

Partager "Studying transmission activation of aphid-vectored Cauliflower mosaic virus"

Copied!
2
0
0

Chargement.... (Voir le texte intégral maintenant)

Texte intégral

(1)

HAL Id: hal-01602777

https://hal.archives-ouvertes.fr/hal-01602777

Submitted on 2 Jun 2020

HAL is a multi-disciplinary open access archive for the deposit and dissemination of sci-entific research documents, whether they are pub-lished or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers.

L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

Studying transmission activation of aphid-vectored

Cauliflower mosaic virus

Beatriz Dader Alonso, Jean Luc Macia, M. Drucker

To cite this version:

Beatriz Dader Alonso, Jean Luc Macia, M. Drucker. Studying transmission activation of aphid-vectored Cauliflower mosaic virus. 3. Hemipteran-Plant Interactions Symposium. HPIS 2017, Jun 2017, Madrid, Spain. 234 p., 2017, HPIS 2017 3rd Hemipteran-Plant Interactions Symposium : book of abstracts. �hal-01602777�

(2)

S 5 H E M I P T E R A - P L A N T P A T H O G E N I N T E R A C T I O N S | 123

Studying transmission activation of aphid-vectored Cauliflower mosaic virus

Dáder B*, Macia JL and Drucker M

INRA, UMR BGPI, France *Corresponding author: beatriz.dader-alonso@inra.fr Poster 59 It has been assumed for a long time that virus acquisition was simple contamination of the stylets occurring when aphids feed on infected plants. However, non-circulative Cauliflower mosaic virus (CaMV) forms during aphid punctures specific transmission morphs in infected cells. More precisely, the CaMV transmission helper protein P2 is dispatched from cytoplasmic transmission body inclusions and virus particles from virus factory inclusions to form together and transiently transmission morphs on microtubules that are acquired and transmitted by the vector. This 'Transmission Activation' (TA), discovered first for CaMV and recently described for the Potyvirus

Turnip mosaic virus, requires that the virus recognizes the presence of aphids via the plant

perception systems, and then induces TA. We want 1) to characterize TA reaction in living tissue after biotic (aphid punctures) and abiotic stresses, and 2) to capture and identify plant partners involved in TA. For this, we chose GFP, which serves as a fluorescent in vivo reporter, and can be used to immunocapture interaction factors by the GFP trap technique. Since the CaMV genome cannot accommodate the entire GFP sequence, we implemented the split GFP system, based on spontaneous auto-assembling of the non-fluorescent small GFP11 (16 amino acids) and big GFP1-10 (rest of the molecule) fragments to fluorescent GFP. A recombinant CaMV with GFP11 fused to P2 was constructed and used to infect transgenic Arabidopsis expressing GFP1-10. The virus was infectious and infected cells displayed P2-GFP fluorescence in transmission body-like structures. When validated, the system might help to identify TA elicitors by tracking GFP11-P2 behavior after application of candidate molecules. The same approach will be used for viral protein P6, with the goal to potentially detect early infection events at the site of aphid-inoculated cells. P6 is particularly suited for this because it is the first viral protein to appear during infection.

Références

Télécharger maintenant ( PDF - 2 Page - 270.02 KB )

Documents relatifs

O n t h e T ( 1 ) - t h e o r e m f o r t h e C a u c h y i n t e g r a l J o a n V e r d e r a A b s t r a c t . T h e m a i n g o a l o f t h i s p a p e r i s t o p r e s e n t a n a l t e r n a t i v e , r e a l v a r i a b l e p r o o f o f t h e T (

Later on the identity (6) was used to obtain estimates from below for analytic capacity [Me] and to describe uniform rectifiability via the mapping properties

O n r e g u l a r i z a t i o n i n B a n a c h s p a c e s T h o m a s S t r S m b e r g 1 . I n t r o d u c t i o n I n t h e p r e s e n t p a p e r w e p r o p o s e a r e g u l a r i z a t i o n p r o c e d u r e f o r f u n c t i o n s d e f i n e d

Last on the agenda, the Lasry-Lions approximates of functions f (defined on Hilbert space) which are locally convex up to a positive multiple of the square of

M a r t i n b o u n d a r i e s o f s e c t o r i a l d o m a i n s M i c h a e l C . C r a n s t o n ( 1 ) a n d T h o m a s S . S a l i s b u r y ( 2 ) A b s t r a c t . L e t D b e a d o m a i n i n l e t 2 w h o s e c o m p l e m e n t i s c o n t a i

This test is related to other integral tests due to Benedicks and Chevallier, the former in the special case of a Denjoy domain.. We then generalise our test,

P a r a b o l i c v e c t o r b u n d l e s o n c u r v e s U . N . B h o s l e S e s h a d r i i n t r o d u c e d t h e n o t i o n o f p a r a b o l i c s t r u c t u r e s o n v e c t o r b u n d l e s 4 a n d l a t e r c o n s t r u c t e d a m o d u

There exists a coarse moduli scheme M for the equivalence classes of semistable parabolic vector bundles (on an irreducible nonsingular complete curve X}.. of

T r a c e s o f p l u r i h a r m o n i c f u n c t i o n s o n c u r v e s B o B e r n d t s s o n a n d J o a q u i m B r u n a A b s t r a c t . W e p r o v e t h a t , i f 7 i s a s i m p l e s m o o t h c u r v e i n t h e u n i t s p h e r e i n C n

We prove that, if 7 is a simple smooth curve in the unit sphere in C n, the space o pluriharmonic functions in the unit ball, continuous up to the boundary, has a

S o m e g o o d u n i r a t i o n a l f a m i l i e s o f s p a c e c u r v e s M e i - C h u C h a n g * T h e p r o b l e m o f c l a s s i f y i n g s p a c e c u r v e s , w h i c h g o e s b a c k t o H a l p h e n a n d N o e t h e r , m a y b e r o

construct a reflexive sheaf from Y using the Serre construction, and then to describe this sheaf by means of a monad, where the family of monads involved

T h 4 o r 6 m e d e p o i n t f i x e e t d ' a p p r o x i m a t i o n B . B e a u z a m y e t P . E n f l o N o t r e b u t d a n s c e t a r t i c l e e s t d e d o n n e r p l u s i e u r s a p p l i c a t i o n s d e l a n o t i o n ( i n t r o - d u

Nous 6nonr ~t la fin du second paragraphe, le r6sultat d'approximation dans le eas particulier des points minimaux, et, dans le troisi6me paragraphe, nous l'appli- quons pour

O n u n i f o r m l y h o m e o m o r p h i c n o r m e d s p a c e s I I M . R i b e T h i s p a p e r c o n t i n u e s t h e s t u d i e s o f t h e s i t u a t i o n w h e n t w o B a n a c h s p a c e s a r e u n i f o r m l y h o m e o m o r p h i c

MANKIEWICZ, P., On the differentiability of Lipschitz mappings in Fr6chet spaces, Studia Math.. MANKIEWICZ, P., On spaces uniformly homeomorphic to Hilbertian Fr6chet