Gbekleyet al., J. Appl. Biosci. 2021 The Togolese Medicinal Recipe, Diabeto-Dolvo® Exerted Antidiabetic Effects in Wistar Rats
Journal of Applied Biosciences 157: 16223 - 16236
ISSN 1997-5902
The Togolese Medicinal Recipe, Diabeto-Dolvo®
Exerted Antidiabetic Effects in Wistar Rats
Holaly Efui Gbekley1, Kokou Idoh2, Nassifatou Titikpina3, Kodjovi Agbodeka1, Kokou Anani1, Gnatoulma Katawa1, Bouraima Djeri1, Tchadjobo Tchacondo1, Simplice DamintotiKarou1, 4*, Yaovi Ameyapoh1, Simpore Jacques4.
1. Ecole Supérieure des Techniques Biologiques et Alimentaires (ESTBA-UL), Université de Lomé, Togo.
2. Laboratoire de Physiologie et de Pharmacologie, Faculté des Sciences (FDS-UL) Université de Lomé, Togo.
3. Laboratoire de Botanique et Ecologie Végétale Faculté des Sciences (FDS-UL) Université de Lomé, Togo.
4. Centre de Recherche de Biologie Moléculaire Pietro-Annigoni (CERBA) Ouagadougou, Burkina-Faso.
Correspondence: Karou Damintoti Simplice, Ecole Supérieure des Techniques Biologiques et Alimentaires (ESTBA- UL), Université de Lomé, 01 BP 1515, Lomé 01, Togo. Email: simplicekarou@hotmail.com
Original submitted in on 24th November 2020. Published online at www.m.elewa.org/journals/ on 31st January 2021 https://doi.org/10.35759/JABs.157.8
ABSTRACT
Objective: this study was to evaluate the toxicity of a herbal recipe Diabeto-Dolvo® (DD) and its efficacy in streptozotocin-induced diabetic Wistar rats.
Methodology and results: The toxicity test was performed by oral administration of the extract to rats while diabetes was induced with streptozotocin, the 250, and 500 mg / kg body weight. The results of the toxicity tests revealed no evidence of mortality or morbidity suggesting an LD50 greater than 5000 mg / kg. Similarly, the biochemical and haematological parameters remained unchanged. In antidiabetic tests, there was a progressive decrease followed by a normalization of the glucose level of the treated rats. Overall, the extract at 250 and 500 mg / kg body weight resulted in a significant reduction in glycated haemoglobin, amylase, lipase, G6PD and serum lipids.
Conclusion and application of results: This study revealed that, the treatment with the recipe might repair oxidative damages, hyperglycaemia and hyperlipidaemia in diabetic rats in the same way as the treatment with glibenclamide. This study is a contribution to the experimental validation of the DD recipe. In research and development application, the DD recipe will be used in the treatment of cases of diabetes mellitus. A natural product, it will support the multiple treatments of so-called conventional medicine, relatively expensive for the population.
Key words: Diabeto-Dolvo®; blood glucose; oxidative damage; streptozotocin; diabetes mellitus; Togo.
